Identification of VHA genes related to organic acid metabolism and functional analysis of key genes in Cerasus humilis

IF 4.2 2区农林科学 Q1 HORTICULTURE Scientia Horticulturae Pub Date : 2025-02-15 DOI:10.1016/j.scienta.2025.114034

Shuai Zhang , Jie Zhang , Lu Zhang , HaoJia Ye , ChenYi Wang , Xiaopeng Mu , Jiancheng Zhang , LuTing Jia , Yu Gary Gao , Pengfei Wang

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Abstract

The content and types of organic acids contribute significantly to the varietal differences in the quality of Cerasus humilis fruits. The molecular mechanisms underlying the accumulation of organic acids in C. humilis remain poorly understood. The study first identified the role of VHA family genes in malic acid metabolism in the low-acid variety '3–19–3′ and the high-acid variety 'Jinou 1′, identifying the VHA genes ChVHA-V0-a3 and ChVHA-V1-G3 that may be related to malic acid metabolism. Furthermore, we conducted extensive validations of the candidate VHA genes in mature fruit of Cerasus humilis 45 accessions, as well as during the fruit development of the low-acid variety '3–6–8′ and the high-acid variety 'Ziou 1′. Finally, the transient transformation technology was utilized to verify the functions of the candidate VHA genes. The results from this study provided new insights into the molecular mechanisms behind the regulation of malic acid production in C. humilis fruits.

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芥蓝有机酸代谢相关VHA基因的鉴定及关键基因的功能分析

有机酸的含量和种类是造成矮樱桃果实品质差异的重要原因。有机酸在葎草中积累的分子机制尚不清楚。本研究首先确定了低酸品种“3-19-3”和高酸品种“吉欧1号”中VHA家族基因在苹果酸代谢中的作用，鉴定出可能与苹果酸代谢有关的VHA基因ChVHA-V0-a3和ChVHA-V1-G3。此外，我们还对候选VHA基因进行了广泛的验证，并在低酸品种“3-6-8”和高酸品种“紫欧1”的果实发育过程中对候选VHA基因进行了验证。最后，利用瞬时转化技术对候选VHA基因的功能进行验证。本研究结果为研究苹果酸产生调控的分子机制提供了新的思路。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.