Effectiveness of Mycobacterium tuberculosis recombinant proteins-coated gold nanoparticles in improving the interferon-gamma release assay test

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES Enfermedades infecciosas y microbiologia clinica Pub Date : 2025-03-01 DOI:10.1016/j.eimc.2024.06.015

Najmeh Jomehpour , Mojtaba Sankian , Saman Soleimanpour , Mohammad Derakhshan , Kiarash Ghazvini , Safora Pordel , Malihe Moghadam

{"title":"Effectiveness of Mycobacterium tuberculosis recombinant proteins-coated gold nanoparticles in improving the interferon-gamma release assay test","authors":"Najmeh Jomehpour , Mojtaba Sankian , Saman Soleimanpour , Mohammad Derakhshan , Kiarash Ghazvini , Safora Pordel , Malihe Moghadam","doi":"10.1016/j.eimc.2024.06.015","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Gold nanoparticles (GNPs) are chemically inert, have low toxicity, and are easy to modify and functionalize for the detection of many pathogens. They have excellent immune modulatory and adjuvant properties. The aim of this study was to improve the diagnosis of latent tuberculosis infection (LTBI) by adding GNPs in tests based on interferon-gamma (IFN-γ) measurement.</div></div><div><h3>Methods</h3><div>GNPs were coated with <em>Mycobacterium tuberculosis</em> (Mtb) recombinant proteins including TB10.4, CFP-10, ESAT-6, and TB7.7. Then, they exposed the whole blood of subjects with active tuberculosis (aTB), LTBI, and healthy controls (HC). Subsequently, IFN-γ produced in GNP tubes (QFT-NG) was measured and compared with IFN-γ produced in tubes without GNPs [QFT-A: (CFP-10, ESAT-6, TB7.7, TB10.4), QFT-B: (CFP-10, ESAT-6, TB7.7)].</div></div><div><h3>Results</h3><div>The results showed that the IFN-γ production in the GNPs tubes was significantly higher than in tubes without GNPs in aTB, LTBI, and HC subjects. In aTB patients, the mean difference (MD) between the QFT-NG and QFT-A tubes was 0.44, with a <em>P</em>-value of 0.04, and a 95% CI: 0.07–0.88. Furthermore, in LTBI individuals, the QFT-NG and QFT-A tubes exhibited an MD=0.21, <em>P</em>-value=0.03, 95% CI: 0.15–0.4. In the aTB and LTBI subjects were detected in the QFT-NG tubes with a sensitivity of 95%, 100% and specificity 100%, 86%, respectively.</div></div><div><h3>Conclusion</h3><div>The use of GNPs coated with Mtb recombinant proteins can aid in detecting LTBI subjects by increasing IFN-γ levels.</div></div>","PeriodicalId":11608,"journal":{"name":"Enfermedades infecciosas y microbiologia clinica","volume":"43 3","pages":"Pages 148-155"},"PeriodicalIF":2.6000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Enfermedades infecciosas y microbiologia clinica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0213005X24002702","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

Gold nanoparticles (GNPs) are chemically inert, have low toxicity, and are easy to modify and functionalize for the detection of many pathogens. They have excellent immune modulatory and adjuvant properties. The aim of this study was to improve the diagnosis of latent tuberculosis infection (LTBI) by adding GNPs in tests based on interferon-gamma (IFN-γ) measurement.

Methods

GNPs were coated with Mycobacterium tuberculosis (Mtb) recombinant proteins including TB10.4, CFP-10, ESAT-6, and TB7.7. Then, they exposed the whole blood of subjects with active tuberculosis (aTB), LTBI, and healthy controls (HC). Subsequently, IFN-γ produced in GNP tubes (QFT-NG) was measured and compared with IFN-γ produced in tubes without GNPs [QFT-A: (CFP-10, ESAT-6, TB7.7, TB10.4), QFT-B: (CFP-10, ESAT-6, TB7.7)].

Results

The results showed that the IFN-γ production in the GNPs tubes was significantly higher than in tubes without GNPs in aTB, LTBI, and HC subjects. In aTB patients, the mean difference (MD) between the QFT-NG and QFT-A tubes was 0.44, with a P-value of 0.04, and a 95% CI: 0.07–0.88. Furthermore, in LTBI individuals, the QFT-NG and QFT-A tubes exhibited an MD = 0.21, P-value = 0.03, 95% CI: 0.15–0.4. In the aTB and LTBI subjects were detected in the QFT-NG tubes with a sensitivity of 95%, 100% and specificity 100%, 86%, respectively.

Conclusion

The use of GNPs coated with Mtb recombinant proteins can aid in detecting LTBI subjects by increasing IFN-γ levels.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

求助全文

约1分钟内获得全文去求助

来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.