Effectiveness of Mycobacterium tuberculosis recombinant proteins-coated gold nanoparticles in improving the interferon-gamma release assay test

IF 2.5 4区医学 Q3 INFECTIOUS DISEASES Enfermedades infecciosas y microbiologia clinica Pub Date : 2025-03-01 Epub Date: 2024-08-02 DOI:10.1016/j.eimc.2024.06.015

Najmeh Jomehpour , Mojtaba Sankian , Saman Soleimanpour , Mohammad Derakhshan , Kiarash Ghazvini , Safora Pordel , Malihe Moghadam

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Abstract

Introduction

Gold nanoparticles (GNPs) are chemically inert, have low toxicity, and are easy to modify and functionalize for the detection of many pathogens. They have excellent immune modulatory and adjuvant properties. The aim of this study was to improve the diagnosis of latent tuberculosis infection (LTBI) by adding GNPs in tests based on interferon-gamma (IFN-γ) measurement.

Methods

GNPs were coated with Mycobacterium tuberculosis (Mtb) recombinant proteins including TB10.4, CFP-10, ESAT-6, and TB7.7. Then, they exposed the whole blood of subjects with active tuberculosis (aTB), LTBI, and healthy controls (HC). Subsequently, IFN-γ produced in GNP tubes (QFT-NG) was measured and compared with IFN-γ produced in tubes without GNPs [QFT-A: (CFP-10, ESAT-6, TB7.7, TB10.4), QFT-B: (CFP-10, ESAT-6, TB7.7)].

Results

The results showed that the IFN-γ production in the GNPs tubes was significantly higher than in tubes without GNPs in aTB, LTBI, and HC subjects. In aTB patients, the mean difference (MD) between the QFT-NG and QFT-A tubes was 0.44, with a P-value of 0.04, and a 95% CI: 0.07–0.88. Furthermore, in LTBI individuals, the QFT-NG and QFT-A tubes exhibited an MD = 0.21, P-value = 0.03, 95% CI: 0.15–0.4. In the aTB and LTBI subjects were detected in the QFT-NG tubes with a sensitivity of 95%, 100% and specificity 100%, 86%, respectively.

Conclusion

The use of GNPs coated with Mtb recombinant proteins can aid in detecting LTBI subjects by increasing IFN-γ levels.

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结核分枝杆菌重组蛋白包覆金纳米颗粒改善干扰素释放试验的有效性

黄金纳米颗粒（GNPs）具有化学惰性，毒性低，易于修饰和功能化，可用于检测许多病原体。它们具有优良的免疫调节和佐剂特性。本研究的目的是通过在基于干扰素-γ (IFN-γ)测量的测试中添加GNPs来提高潜伏性结核感染（LTBI）的诊断。方法采用结核分枝杆菌TB10.4、CFP-10、ESAT-6、TB7.7重组蛋白包被gnps。然后，他们暴露活动性肺结核（aTB）、LTBI和健康对照（HC）受试者的全血。随后，测量GNP管中产生的IFN-γ (QFT-NG)并与没有GNPs管中产生的IFN-γ进行比较[QFT-A: (CFP-10, ESAT-6, TB7.7, TB10.4), QFT-B: (CFP-10, ESAT-6, TB7.7)]。结果在aTB、LTBI和HC组中，GNPs管中IFN-γ的产生明显高于不含GNPs的管。在aTB患者中，QFT-NG和QFT-A管的平均差异（MD）为0.44，p值为0.04,95% CI为0.07-0.88。此外，在LTBI个体中，QFT-NG和QFT-A管的MD = 0.21， p值= 0.03,95% CI: 0.15-0.4。在aTB和LTBI受试者中，QFT-NG管检测的灵敏度分别为95%，100%，特异性分别为100%，86%。结论Mtb重组蛋白包被GNPs可以通过提高IFN-γ水平来检测LTBI患者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Enfermedades infecciosas y microbiologia clinica 医学-传染病学

CiteScore

2.10

自引率

8.00%

发文量

194

审稿时长

29 days

期刊介绍： Hoy está universalmente reconocida la renovada y creciente importancia de la patología infecciosa: aparición de nuevos agentes patógenos, de cepas resistentes, de procesos con expresión clínica hasta ahora desconocida, de cuadros de una gran complejidad. Paralelamente, la Microbiología y la Infectología Clínicas han experimentado un gran desarrollo como respuesta al reto planteado por la actual patología infecciosa. Enfermedades Infecciosas y Microbiología Clínica es la Publicación Oficial de la Sociedad Española SEIMC. Cumple con la garantía científica de esta Sociedad, la doble función de difundir trabajos de investigación, tanto clínicos como microbiológicos, referidos a la patología infecciosa, y contribuye a la formación continuada de los interesados en aquella patología mediante artículos orientados a ese fin y elaborados por autores de la mayor calificación invitados por la revista.