Single-electrode electrochemiluminescence immunoarray combined with smartphone for high-throughput detection of heart-type fatty acid binding protein

Abstract

Acute myocardial infarction (AMI) is a cardiovascular disease with high fatality and acute morbidity. The timely diagnosis and treatment are vital for improving recovery of AMI patients. Heart-type fatty acid binding protein (h-FABP) is an important biomarker for early assessment and exclusion of AMI. Herein, we developed single-electrode electrochemiluminescence (ECL) immunoarray for high throughput and portable detection of h-FABP. This ECL immunoarray was fabricated by successively assembling ECL nanocomposites and h-FABP conjugated gold nanoparticles on the surface of indium tin oxide microchips electrodes and the bovine serum albumin was used in blocking nonspecific binding sites, which can be used for detection of h-FABP. In the presence of h-FABP, the ECL intensity increased with increasing concentration. Thus, a high throughput and portable immunoarray was established for the determination of h-FABP with a linear dynamic range from 0.1 to 1000 pg/mL and a detection limit of 0.09 pg/mL, which exhibited excellent performance compared with other ECL methods. Moreover, the developed ECL immunoarray was used in detecting the h-FABP in clinical sample of human serum, which demonstrated good recoveries in the range of 92.20 %∼108.00 %. Therefore, the proposed ECL immunoarray can effectively handle the determination of clinical samples, which was promising for using the early screening or exclusion for AMI at home or in an ambulance due to portable detection based on smartphone. This strategy is very promising to effectively reduce fatality and acute morbidity of AMI patients.