NiWO4 with oxygen vacancy induced Au nanoparticle signal amplification for electrochemical detection of swine fever virus

Abstract

Classical swine fever virus (CSFV) represents a significant threat to the pig industry, capable of causing devastating infectious diseases.. It is an urgent need to identify and quantify viruses with a rapid, sensitive and accurate technology. Integration of nanotechnology will allow the development of microdevices combined with electrochemical technology for efficient virus detection. Here, the electrochemical characteristics of MWCNTs-decorated Au-NiWO₄ are studied, and an ideal nanomaterial is constructed for immunosensor of CSFV antigen identifying based on steric effects about oxygen vacancy coupling CSFV enhanced reactant adsorption. The developed immunosensor detects CSFV over a range of 2 × 10^-13μg·mL⁻¹-8 × 10^-9μg·mL⁻¹, and with a detection limit of 1.58 × 10^-13 μg·mL⁻¹. In addition, AuNPs/NiWO₄/Ć shows an excellent linear relationship at the scanning speed of 10 ∼ 100 mv/s, and the relative standard deviation (RSD) was found to be only 0.60 %. The synergistic integration of MWCNTs/GO, AuNPs and electroactive Au-NiWO₄ provides superior signal response when used for the quantification of CSFV from CSFV antigen samples, suggesting the application prospects of this nanomaterial in detection.