Evaluation of a DNA vector plasmid encoding a partial rop18 gene from toxoplasma gondii in domestic cats as a vaccine candidate

Abstract

The present study aimed to evaluate a DNA vector plasmid encoding a partial rop18 gene from Toxoplasma gondii in domestic cats as a potential vaccine candidate. Four domestic cats (Felis catus) were used, of which two animals received 25 μg of pcDNA 3.1 + rop18, and two received 25 μg of pcDNA 3.1. All animals received intramuscular immunizations with four doses every three weeks along with 1.5 % levamisole. Thirty days after the last immunization, the animals were infected with 300 tissue cysts from ToxoDB #182 strain, a non-archetypal genotype isolated from a wild cat. Fecal examinations were performed for oocyst shedding. Enzyme-linked immunosorbent assay and western blotting analyses with recombinant ROP18 were performed to assess the humoral immune response. Animals that received plasmid containing the partial T. gondii rop18 gene produced specific IgG antibodies and shed 53.3 % fewer oocysts than controls. The two groups of animals showed no statistically significant differences (p > 0.05) in oocyst shedding; however, they showed significant differences in the detection of anti-Toxoplasma antibodies (p < 0.05). In conclusion, the T. gondii rop18 gene is a potential vaccine candidate against oocyst shedding in cats.