Insight into the mechanisms of methane production enhancement by bioaugmentation with H2/CO2 in anaerobic digestion

IF 8.1 2区工程技术 Q1 CHEMISTRY, PHYSICAL International Journal of Hydrogen Energy Pub Date : 2025-03-05 DOI:10.1016/j.ijhydene.2025.02.420

Jiancheng Yang , Haojun Yang , Tao Xing , Yongming Sun , Bin Qu , Lianhua Li , Ying Li , Ying Guo , Feng Zhen , Yuwan Pang

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Abstract

In this study, the high performance hydrogenotrophic methanogenic bacteria were domesticated at different H₂/CO₂ molar ratios, and the influence of the addition of high performance hydrogenotrophic methanogenic bacteria on the methanogenic performance of the anaerobic digestion (AD) system and the change of microbial community structure were investigated. 16s rDNA showed that Methanobrevibacter was the dominant methanogenic bacterium at different methane production experiment results showed H₂/CO₂ molar ratios, and the maximum cumulative methane production of 220.87 ± 6.55 mL/g VS was obtained in the bioaugmentation experiments when using a domesticating agent with an H₂/CO₂ molar ratio of 4. In addition, the domesticating agent enhanced the hydrolysis and acidification steps, and changed the microbial community structure. This study demonstrated the potential of different H₂/CO₂ molar ratios for the domestication of high-performance hydrogen-based methanogenic bacteria.It would lay the foundation for the development of high-load anaerobic digestion in the future, and had great significance for the efficient and stable operation of biogas and biogas engineering.

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来源期刊

International Journal of Hydrogen Energy 工程技术-环境科学

CiteScore

13.50

自引率

25.00%

发文量

3502

审稿时长

60 days

期刊介绍： The objective of the International Journal of Hydrogen Energy is to facilitate the exchange of new ideas, technological advancements, and research findings in the field of Hydrogen Energy among scientists and engineers worldwide. This journal showcases original research, both analytical and experimental, covering various aspects of Hydrogen Energy. These include production, storage, transmission, utilization, enabling technologies, environmental impact, economic considerations, and global perspectives on hydrogen and its carriers such as NH3, CH4, alcohols, etc. The utilization aspect encompasses various methods such as thermochemical (combustion), photochemical, electrochemical (fuel cells), and nuclear conversion of hydrogen, hydrogen isotopes, and hydrogen carriers into thermal, mechanical, and electrical energies. The applications of these energies can be found in transportation (including aerospace), industrial, commercial, and residential sectors.