Insight into the mechanisms of methane production enhancement by bioaugmentation with H2/CO2 in anaerobic digestion

IF 8.3 2区工程技术 Q1 CHEMISTRY, PHYSICAL International Journal of Hydrogen Energy Pub Date : 2025-03-27 Epub Date: 2025-03-05 DOI:10.1016/j.ijhydene.2025.02.420

Jiancheng Yang , Haojun Yang , Tao Xing , Yongming Sun , Bin Qu , Lianhua Li , Ying Li , Ying Guo , Feng Zhen , Yuwan Pang

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Abstract

In this study, the high performance hydrogenotrophic methanogenic bacteria were domesticated at different H₂/CO₂ molar ratios, and the influence of the addition of high performance hydrogenotrophic methanogenic bacteria on the methanogenic performance of the anaerobic digestion (AD) system and the change of microbial community structure were investigated. 16s rDNA showed that Methanobrevibacter was the dominant methanogenic bacterium at different methane production experiment results showed H₂/CO₂ molar ratios, and the maximum cumulative methane production of 220.87 ± 6.55 mL/g VS was obtained in the bioaugmentation experiments when using a domesticating agent with an H₂/CO₂ molar ratio of 4. In addition, the domesticating agent enhanced the hydrolysis and acidification steps, and changed the microbial community structure. This study demonstrated the potential of different H₂/CO₂ molar ratios for the domestication of high-performance hydrogen-based methanogenic bacteria.It would lay the foundation for the development of high-load anaerobic digestion in the future, and had great significance for the efficient and stable operation of biogas and biogas engineering.

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厌氧消化中H2/CO2生物强化提高甲烷产量的机理研究

本研究通过在不同H2/CO2摩尔比下驯化高产产氢营养型产甲烷菌，考察高产产氢营养型产甲烷菌的添加对厌氧消化（AD）系统产甲烷性能的影响以及微生物群落结构的变化。16s rDNA结果表明，在不同产甲烷量条件下，产甲烷菌为优势产甲烷菌；在H2/CO2摩尔比条件下，使用H2/CO2摩尔比为4的驯化剂进行生物强化实验时，累计产甲烷量达到220.87±6.55 mL/g VS。此外，驯化剂增强了水解和酸化步骤，改变了微生物群落结构。本研究证明了不同H2/CO2摩尔比对高效氢基产甲烷菌驯化的潜力。这将为今后高负荷厌氧消化的发展奠定基础，对沼气的高效稳定运行和沼气工程具有重要意义。

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来源期刊

International Journal of Hydrogen Energy 工程技术-环境科学

CiteScore

13.50

自引率

25.00%

发文量

3502

审稿时长

60 days

期刊介绍： The objective of the International Journal of Hydrogen Energy is to facilitate the exchange of new ideas, technological advancements, and research findings in the field of Hydrogen Energy among scientists and engineers worldwide. This journal showcases original research, both analytical and experimental, covering various aspects of Hydrogen Energy. These include production, storage, transmission, utilization, enabling technologies, environmental impact, economic considerations, and global perspectives on hydrogen and its carriers such as NH3, CH4, alcohols, etc. The utilization aspect encompasses various methods such as thermochemical (combustion), photochemical, electrochemical (fuel cells), and nuclear conversion of hydrogen, hydrogen isotopes, and hydrogen carriers into thermal, mechanical, and electrical energies. The applications of these energies can be found in transportation (including aerospace), industrial, commercial, and residential sectors.