A novel method for detecting Lipoarabinomannan in urine with the promise of meeting the WHO target product profile for the diagnosis of tuberculosis

IF 2.9 3区医学 Q3 IMMUNOLOGY Tuberculosis Pub Date : 2025-02-24 DOI:10.1016/j.tube.2025.102619

Katharina Budde , Christoph Lange , Maja Reimann , Nika Zielinski , Lennard Meiwes , Niklas Köhler

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Abstract

The diagnosis of tuberculosis largely relies on the detection of Mycobacterium tuberculosis (M. tuberculosis) via pathogen-specific DNA or bacterial culture from sputum samples. As the only point-of-care test so far, urinary lipoarabinomannan (LAM) has been endorsed by the World Health Organization for the diagnosis of tuberculosis in people living with HIV.

In this study, the electrochemiluminescence LAM research assay (EclLAM) was used to measure LAM in the urine of HIV-sero-negative individuals with pulmonary tuberculosis and to monitor anti-tuberculosis treatment. Urine samples from 18 patients with microbiologically confirmed tuberculosis were analyzed before and after the initiation of anti-tuberculosis therapy and 17 healthy controls via the S4-20/A194-01 antibody pair.

The assay identified 13/18 (72.2 %) patients with tuberculosis and was negative in 17/17 (100.0 %) controls (AUC 0.88). The results of the reactive urine LAM tests correlated with the detection of M. tuberculosis growth in culture (r = 0.94, p < 0.05).

In conclusion, the LAM-specific antibody assay is promising to fulfill the WHO target product profile for the diagnosis of tuberculosis.

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一种检测尿液中脂arabinman聚糖的新方法，有望满足世卫组织诊断结核病的目标产品概况

结核病的诊断在很大程度上依赖于通过病原体特异性DNA或痰样本细菌培养检测结核分枝杆菌（M. tuberculosis）。作为迄今为止唯一的即时检测方法，尿脂阿拉伯糖甘露聚糖（LAM）已被世界卫生组织认可用于诊断艾滋病毒感染者的结核病。本研究采用电化学发光法（EclLAM）检测hiv血清阴性肺结核患者尿液中的LAM，并监测抗结核治疗情况。对18例微生物学证实的结核病患者和17例健康对照者在开始抗结核治疗前后的尿液样本进行了S4-20/A194-01抗体对分析。该方法鉴定出13/18（72.2%）的肺结核患者，17/17（100.0%）的对照（AUC 0.88）为阴性。尿反应性LAM检测结果与培养物结核分枝杆菌生长检出率相关(r = 0.94, p <；0.05)。总之，lam特异性抗体测定有望满足世卫组织诊断结核病的目标产品概况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.