Structural characterization and in vitro hypolipidemic and hypoglycemic activities of a novel neutral polysaccharide from Siraitia grosvenorii (Luo Han Guo) flowers

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Chemical and Biological Technologies in Agriculture Pub Date : 2025-03-06 DOI:10.1186/s40538-025-00750-w

Hao Teng, Limei Qiu, Zhizhi Huang, Sam Al-Dalali, Cailin Li, Aiying Xu, Zhigui He

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Abstract

Siraitia grosvenorii, commonly known as Luo Han Guo, is a medicinal and edible plant whose flowers contain bioactive polysaccharides with underexplored therapeutic potential. This study isolated a novel polysaccharide fraction (SGFP-2) from Siraitia grosvenorii flowers through DEAE-Crystarose Fast Flow chromatography. Structural analysis revealed SGFP-2 is a heteropolysaccharide with average molecular weight of 1.67 × 10⁵ Da and composed of Rha, Glc, Gal, GlcA, GalA, Man, and Ara with a molar ratio of 8.17:1.54:60.06:3.41:5.37:3.54:15.44. Methylation analysis identified dominant glycosidic linkages of SGFP-2 as → 6)-Galp-(1 → (30.42%), Galp-(1 → (22.69%), Araf-(1 → (18.29%), Rhap-(1 → (8.70%), → 3,6)-Galp-(1 → (8.54%). Scanning electron microscopy and Congo red staining results showed that the network structure of SGFP-2 was lamellar without trihelix conformation. In vitro experiments have revealed that SGFP-2 possesses lipid-binding capacity, bile salt adsorption properties, and potent inhibitory activity against crucial enzymes involved in glucose-lipid metabolism, specifically pancreatic lipase, cholesterol esterase, α-amylase, and α-glucosidase. These findings suggest that SGFP-2 demonstrates potential hypolipidemic and hypoglycemic effects in vitro. This study provides preliminary evidence to support further development and utilization of Siraitia grosvenorii flower polysaccharides.

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罗汉果花中一种新型中性多糖的结构表征及其体外降血脂和降糖活性

罗汉果，俗称罗汉果，是一种药用和食用植物，其花含有生物活性多糖，治疗潜力尚未开发。采用deae -结晶糖快速流动色谱法从罗汉果花中分离到一种新的多糖（SGFP-2）。结构分析表明，SGFP-2是一种平均分子量为1.67 × 105 Da的杂多糖，由Rha、Glc、Gal、GlcA、GalA、Man和Ara组成，摩尔比为8.17:1.54:60 0.06:3.41:5.37:3.54:15.44。甲基化分析确定主导糖苷的SGFP-2联系→6)Galp -(1→(30.42%)、Galp -(1→(22.69%)、Araf -(1→(18.29%),世行-(1→(8.70%)→3、6)Galp -(1→(8.54%)。扫描电镜和刚果红染色结果显示，SGFP-2的网络结构为片层状，无三螺旋构象。体外实验表明，sgp -2具有脂质结合能力、胆盐吸附特性，并对参与糖脂代谢的关键酶，特别是胰脂肪酶、胆固醇酯酶、α-淀粉酶和α-葡萄糖苷酶具有有效的抑制活性。这些发现表明，SGFP-2在体外具有潜在的降血脂和降血糖作用。本研究为罗汉果花多糖的进一步开发利用提供了初步依据。图形抽象

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.