{"title":"<i>AoMYB114</i> transcription factor regulates anthocyanin biosynthesis in the epidermis of tender asparagus stems.","authors":"Yanwen Li, Mengyao Li, Zheng Guo, Junting Liu, Peiran Chen, Wei Lu, Chengyao Jiang, Jiachang Xiao, Fengyun Lei, Yangxia Zheng","doi":"10.3389/fpls.2025.1531574","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Asparagus is a valuable vegetable, and its edible part is a tender stem. The color of the tender stem epidermis is an important trait. In particular, purple asparagus is rich in anthocyanins. However, the molecular mechanisms underlying anthocyanin accumulation in purple asparagus remains unclear.</p><p><strong>Methods: </strong>The white variety 'Jinguan' (JG), the green variety 'Fengdao 2' (FD), and the purple variety 'Jingzilu 2' (JZ) were compared using physiological and transcriptomic analysis. High-performance liquid chromatography and real-time quantitative polymerase chain reaction were employed to detect anthocyanins and validate gene expression.</p><p><strong>Results: </strong>Cyanidin 3-glucoside and cyanidin 3-rutinoside were detected as the main anthocyanins in JZ. Transcriptome data demonstrated that 4,694 and 9,427 differentially expressed genes (DEGs) were detected in the JZ versus FD and JZ versus JG control groups, respectively. These DEGs were significantly enriched in pathways associated with anthocyanin accumulation, including phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. A total of 29 structural genes related to anthocyanin biosynthesis were identified. The expression of these structural genes was higher in JZ than in FD and JG, thereby activating the anthocyanin biosynthesis pathway. Additionally, a candidate gene, <i>AoMYB114</i>, was identified based on transcriptomic data. The expression of <i>AoMYB114</i> was associated with anthocyanin accumulation in different tissues. Further research found that overexpression of <i>AoMYB114</i> activated the anthocyanin biosynthesis pathway. It promoted leaf pigment accumulation in transgenic <i>Arabidopsis</i>.</p><p><strong>Discussion: </strong>These findings demonstrate that <i>AoMYB114</i> positively regulated anthocyanin biosynthesis. This study elucidates the molecular mechanism underlying purple coloration in asparagus. It provides important insights for improving asparagus quality and for breeding high-anthocyanin varieties.</p>","PeriodicalId":12632,"journal":{"name":"Frontiers in Plant Science","volume":"16 ","pages":"1531574"},"PeriodicalIF":4.1000,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876374/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Plant Science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fpls.2025.1531574","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction: Asparagus is a valuable vegetable, and its edible part is a tender stem. The color of the tender stem epidermis is an important trait. In particular, purple asparagus is rich in anthocyanins. However, the molecular mechanisms underlying anthocyanin accumulation in purple asparagus remains unclear.
Methods: The white variety 'Jinguan' (JG), the green variety 'Fengdao 2' (FD), and the purple variety 'Jingzilu 2' (JZ) were compared using physiological and transcriptomic analysis. High-performance liquid chromatography and real-time quantitative polymerase chain reaction were employed to detect anthocyanins and validate gene expression.
Results: Cyanidin 3-glucoside and cyanidin 3-rutinoside were detected as the main anthocyanins in JZ. Transcriptome data demonstrated that 4,694 and 9,427 differentially expressed genes (DEGs) were detected in the JZ versus FD and JZ versus JG control groups, respectively. These DEGs were significantly enriched in pathways associated with anthocyanin accumulation, including phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. A total of 29 structural genes related to anthocyanin biosynthesis were identified. The expression of these structural genes was higher in JZ than in FD and JG, thereby activating the anthocyanin biosynthesis pathway. Additionally, a candidate gene, AoMYB114, was identified based on transcriptomic data. The expression of AoMYB114 was associated with anthocyanin accumulation in different tissues. Further research found that overexpression of AoMYB114 activated the anthocyanin biosynthesis pathway. It promoted leaf pigment accumulation in transgenic Arabidopsis.
Discussion: These findings demonstrate that AoMYB114 positively regulated anthocyanin biosynthesis. This study elucidates the molecular mechanism underlying purple coloration in asparagus. It provides important insights for improving asparagus quality and for breeding high-anthocyanin varieties.
期刊介绍：
In an ever changing world, plant science is of the utmost importance for securing the future well-being of humankind. Plants provide oxygen, food, feed, fibers, and building materials. In addition, they are a diverse source of industrial and pharmaceutical chemicals. Plants are centrally important to the health of ecosystems, and their understanding is critical for learning how to manage and maintain a sustainable biosphere. Plant science is extremely interdisciplinary, reaching from agricultural science to paleobotany, and molecular physiology to ecology. It uses the latest developments in computer science, optics, molecular biology and genomics to address challenges in model systems, agricultural crops, and ecosystems. Plant science research inquires into the form, function, development, diversity, reproduction, evolution and uses of both higher and lower plants and their interactions with other organisms throughout the biosphere. Frontiers in Plant Science welcomes outstanding contributions in any field of plant science from basic to applied research, from organismal to molecular studies, from single plant analysis to studies of populations and whole ecosystems, and from molecular to biophysical to computational approaches.
Frontiers in Plant Science publishes articles on the most outstanding discoveries across a wide research spectrum of Plant Science. The mission of Frontiers in Plant Science is to bring all relevant Plant Science areas together on a single platform.
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