Genomic and morphological features of an Amazonian Bacillus thuringiensis with mosquito larvicidal activity.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2025-03-05 DOI:10.1186/s13568-025-01850-4

Veranilce Alves Muniz, Ricardo de Melo Katak, Lílian Caesar, Juan Campos de Oliveira, Elerson Matos Rocha, Marta Rodrigues de Oliveira, Gilvan Ferreira da Silva, Rosemary Aparecida Roque, Osvaldo Marinotti, Olle Terenius, Edmar Vaz de Andrade

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Abstract

The occurrence of mosquito-borne diseases is increasing, and their geographical range is expanding due to climate change. New control measures are urgently needed to combat these debilitating and, in some cases, fatal diseases. Bacteria of the genus Bacillus are of interest due to the production of bioactive compounds, including those useful for insect control. The discovery and characterization of new species of Bacillus with mosquito larvicidal activity may offer opportunities to develop new products for vector control. In this study, we evaluated larvicidal activity, described morphological characteristics, and sequenced and analyzed the genome of a bacterial strain (GD02.13) isolated from the Amazon region. The metabolites produced by GD02.13 are as effective in killing Aedes aegypti larvae as the commercial product Natular™ DT (Spinosad). Furthermore, the morphological characteristics of the GD02.13 spores and crystal inclusions resemble those previously described for B. thuringiensis. A phylogenetic analysis based on 443 single-copy orthologs indicated that the bacterial strain GD02.13 belongs to the Bacillus thuringiensis species. Its genome, which was assembled and has a size of 6.6 Mb, contains 16 secondary metabolite biosynthetic gene clusters and genes encoding insecticidal proteins, predicted based on sequence similarity. The data obtained in this study support the development of new insecticide products based on the strain GD02.13 of B. thuringiensis.

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来源期刊

AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

7.20

自引率

2.70%

发文量

141

审稿时长

13 weeks

期刊介绍： AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.