Antiviral Cellulose-Lignin Assembly Entirely Composed of Plant Cell Wall Components

IF 3.3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Applied Biochemistry and Biotechnology Pub Date : 2025-03-07 DOI:10.1007/s12010-025-05184-6

Chihiro Kimura, Eriko Ohgitani, Osam Mazda, Takashi Watanabe

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Abstract

Antiviral lignin, designated as FR₂₀₀, was produced using acidic microwave glycerolysis of sugarcane bagasse. The lignin strongly inhibited infection by the human norovirus surrogate, feline calicivirus (FCV) without substantial cytotoxicity. The antiviral activity emerged through direct contact of the lignin with the virion. To develop antiviral fibers, cotton was coated with the antiviral lignin AIF₂₀₀, a crude fraction obtained in the process of FR₂₀₀ preparation. Autofluorescence microscope images confirmed successful even coating of the lignin on the cellulose fiber surfaces. ATR FT-IR spectra supported the incorporation of the lignin. The strong anti-FCV activity of the cellulose-lignin assembly was observed by measuring viral titers of the filtrate passing through the lignin-immobilized cotton. The antiviral material developed in this study, totally composed of lignocellulosic biomass, is potentially useful for protecting our health by capturing virions in daily life and livestock environments.

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完全由植物细胞壁成分组成的抗病毒纤维素-木质素组合物。

以甘蔗渣为原料，采用微波酸性甘油水解法制备了抗病木质素FR200。木质素对人诺如病毒替代物猫杯状病毒（FCV）的感染有较强的抑制作用，但无明显的细胞毒性。其抗病毒活性是通过木质素与病毒粒子直接接触而产生的。为了开发抗病毒纤维，在棉花表面涂覆抗病毒木质素AIF200，这是制备FR200过程中得到的粗馏分。自体荧光显微镜图像证实了木质素在纤维素纤维表面的均匀涂层。ATR - FT-IR光谱支持木质素的掺入。通过测定通过木质素固定化棉花的滤液的病毒滴度，观察到纤维素-木质素组合具有很强的抗fcv活性。本研究开发的抗病毒材料完全由木质纤维素生物质组成，通过捕获日常生活和牲畜环境中的病毒粒子来保护我们的健康，具有潜在的用途。

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来源期刊

Applied Biochemistry and Biotechnology 工程技术-生化与分子生物学

CiteScore

5.70

自引率

6.70%

发文量

460

审稿时长

5.3 months

期刊介绍： This journal is devoted to publishing the highest quality innovative papers in the fields of biochemistry and biotechnology. The typical focus of the journal is to report applications of novel scientific and technological breakthroughs, as well as technological subjects that are still in the proof-of-concept stage. Applied Biochemistry and Biotechnology provides a forum for case studies and practical concepts of biotechnology, utilization, including controls, statistical data analysis, problem descriptions unique to a particular application, and bioprocess economic analyses. The journal publishes reviews deemed of interest to readers, as well as book reviews, meeting and symposia notices, and news items relating to biotechnology in both the industrial and academic communities. In addition, Applied Biochemistry and Biotechnology often publishes lists of patents and publications of special interest to readers.