Xueke Jia, Xin Liu, Taomin Zhu, Xiaohan Ma, Ruiming Chen, Huimin Li, Yaping Feng, Liwu Zheng, Yu Liu, Jin Ke
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引用次数: 0
Abstract
Objective: Recent research indicates that macrophages in ganglia are linked to chronic pain, with Piezo2 ion channels playing a key role in pain sensation. Our study aims to elucidate the interplay between macrophages and Piezo2 in temporomandibular joint osteoarthritis (TMJOA) chronic pain.
Materials and methods: We induced TMJOA chronic pain in rats via articular injection of monosodium iodoacetate (MIA). We then depleted macrophages using clodronate liposomes and overexpressed Piezo2 in trigeminal ganglion (TG) neurons with adeno-associated virus 9 (AAV9)-Piezo2 in TMJOA rats. To explore the connection between macrophages and Piezo2, we employed immunofluorescence, in vitro studies, and the Rat Grimace Scale (RGS) to evaluate pain thresholds in TMJOA rats.
Results: A positive correlation was observed between macrophage infiltration and Piezo2 upregulation in TG neurons of TMJOA rats. Depletion of infiltrated macrophages downregulated Piezo2 in TG neurons, while Piezo2 overexpression negated the pain-relieving effects of infiltrated macrophage depletion in TMJOA rats. Macrophages primarily influenced Piezo2 expression in IB4 + - TG neurons of TMJOA chronic pain rats. Ex vivo studies revealed that infiltrated macrophage-derived IL-1β and TNF-α cytokines activate Dil + -TG neurons by upregulating Piezo2 in TMJOA rats.
Conclusions: Infiltrated macrophages exacerbate MIA-induced TMJOA chronic pain by upregulating Piezo2 expression in IB4+-TG neurons.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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