{"title":"Unlocking the potential of genome editing in agriculture with tissue culture-free techniques","authors":"Manman Hu, Degao Liu","doi":"10.1111/nph.70046","DOIUrl":null,"url":null,"abstract":"Genome editing holds great promise for enhancing crop traits; however, progress has been slow due to inefficient delivery methods and reliance on tissue culture for regenerating edited plants, which are time-consuming and labor-intensive. To address these limitations, innovative tissue culture-free techniques have been developed, including meristem editing through biolistic-mediated delivery and RNA virus-mediated delivery. New methods for <i>de novo</i> gene-edited meristem induction and root suckering-based cut–dip–budding have also been established. While these approaches show promise, each faces challenges that must be addressed for practical application in crop improvement. We discuss the transformative potential of these techniques for crop improvement and emphasize the need for ongoing research to refine them and maximize their agricultural impact.","PeriodicalId":214,"journal":{"name":"New Phytologist","volume":"53 1","pages":""},"PeriodicalIF":8.3000,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/nph.70046","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Genome editing holds great promise for enhancing crop traits; however, progress has been slow due to inefficient delivery methods and reliance on tissue culture for regenerating edited plants, which are time-consuming and labor-intensive. To address these limitations, innovative tissue culture-free techniques have been developed, including meristem editing through biolistic-mediated delivery and RNA virus-mediated delivery. New methods for de novo gene-edited meristem induction and root suckering-based cut–dip–budding have also been established. While these approaches show promise, each faces challenges that must be addressed for practical application in crop improvement. We discuss the transformative potential of these techniques for crop improvement and emphasize the need for ongoing research to refine them and maximize their agricultural impact.
期刊介绍：
New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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