Ultrasound-assisted deep eutectic solvent extraction of kukoamine A and kukoamine B from Lycii cortex

IF 1.3 4区生物学 Q4 CHEMISTRY, MEDICINAL Phytochemistry Letters Pub Date : 2025-03-08 DOI:10.1016/j.phytol.2025.02.013

Xiaomei Wang , Jinxia Hu , Ling Ma , Zhiming Han , Jianfei Liu , Zhibin Lu , Dong Pei , Ningli Wang , Duolong Di

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引用次数: 0

Abstract

Deep eutectic solvents (DES), are new environmentally friendly and efficient extraction solvents, and have considerable importance for environmental protection and sustainable development. In this study, ultrasound-assisted extraction (UAE) using DES was carried out to extract kukoamine A (KuA) and kukoamine B (KuB) from Lycii cortex (LyC). The DES was synthesised using a magnetic stirring method. A novel approach to extract KuA and KuB from LyC was developed by combining UAE with DES. Different DES formulations were tested to evaluate the efficiency of KuA and KuB extraction. Among them, choline chloride-lactic acid (ChCl-LA) DES had the best effect, and its extraction rate was much higher than that of methanol, which was 8.5 times that of methanol. Both single factorial experiments and response surface methodology (RSM) were used to optimise and model the extraction process. Density functional theory was also used to discuss the extraction mechanism. It was found that HBD and HBA will interact with hydrogen bonding and van der Waals interactions during the formation of DES, and these interactions will also affect the decrease in electron density. Taking ChCl-LA and ChCl-urea as examples, it was found that the binding energy of ChCl-LA was lower than that of ChCl-urea, indicating that ChCl-LA had a stronger interaction with the target substance and a higher extraction rate. The combination of UAE-DES proved to be a sustainable, effective, and simple extraction technique for the recovery of KuA and KuB from LyC.

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来源期刊

Phytochemistry Letters 生物-生化与分子生物学

CiteScore

3.00

自引率

11.80%

发文量

190

审稿时长

34 days

期刊介绍： Phytochemistry Letters invites rapid communications on all aspects of natural product research including: • Structural elucidation of natural products • Analytical evaluation of herbal medicines • Clinical efficacy, safety and pharmacovigilance of herbal medicines • Natural product biosynthesis • Natural product synthesis and chemical modification • Natural product metabolism • Chemical ecology • Biotechnology • Bioassay-guided isolation • Pharmacognosy • Pharmacology of natural products • Metabolomics • Ethnobotany and traditional usage • Genetics of natural products Manuscripts that detail the isolation of just one new compound are not substantial enough to be sent out of review and are out of scope. Furthermore, where pharmacology has been performed on one new compound to increase the amount of novel data, the pharmacology must be substantial and/or related to the medicinal use of the producing organism.