Trehalose-powered membraneless enzymatic fuel cell based on flexible alginate composite hydrogel bioelectrodes

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Process Biochemistry Pub Date : 2025-03-07 DOI:10.1016/j.procbio.2025.03.003

Said Nawab , Chong Sha , Syed Bilal Shah , Hareef Ahmed Keerio , Zhiguang Zhu , Yang-Chun Yong

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Abstract

This study investigated the design and optimization of a membraneless flexible gel-type enzymatic biofuel cell (EBFC) driven by trehalose for electricity generation. The EBFC was fabricated by printing bioelectrodes on non-woven fabrics encapsulating the enzymes in alginate composite hydrogel, and a conductive carbon layer was incorporated to improve electrochemical transfer. Optimization of trehalase loading revealed that a maximum power density of 4.69 μW/cm² was obtained with a trehalase loading of 3.5 U per 6 mL of 50 mM trehalose. Further optimization of the GOx-to-hydrogel ratio in the anode resulted in a maximum power density of 7.45 μW/cm². Evaluations at different trehalose concentrations demonstrated proportionate increases in voltage, power density, and current density, achieving the highest power density of 10.74 μW/cm² at 100 mM. Connecting three EBFCs in series considerably enhanced power output, achieving a four-fold improvement in power density to 41.32 μW/cm² compared to a single cell. The EBFC also generated power from cockroach hemolymph, with a maximum power density of 8.05 μW/cm². These results highlight that this flexible gel-type EBFC has the potential to be employed in the development of both trehalose-powered and glucose-powered EBFC.

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基于柔性海藻酸盐复合水凝胶生物电极的海藻糖动力无膜酶燃料电池

本研究研究了海藻糖驱动的无膜柔性凝胶型酶生物燃料电池（EBFC）的设计和优化。将酶包埋在海藻酸盐复合水凝胶中，在无纺布上打印生物电极制备EBFC，并加入导电碳层以改善电化学传递。海藻糖酶负载优化结果表明，当海藻糖酶负载为3.5 U / 6 mL（50 mM）海藻糖时，功率密度可达4.69 μW/cm2。进一步优化阳极中氧化石墨烯与水凝胶的比例，得到的最大功率密度为7.45 μW/cm2。在不同海藻糖浓度下的评价表明，电压、功率密度和电流密度成比例地增加，在100 mM时达到10.74 μW/cm2的最高功率密度。串联三个EBFCs大大提高了功率输出，与单个电池相比，功率密度提高了4倍，达到41.32 μW/cm2。EBFC也可以利用蟑螂血淋巴发电，最大功率密度为8.05 μW/cm2。这些结果表明，这种柔性凝胶型EBFC有潜力用于开发海藻糖和葡萄糖驱动的EBFC。

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Laccase from Trametes versicolor

来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.