Process Biochemistry最新文献

Interaction between the electrochemical properties of powdered activated carbon and the biochemical processes within bacteria in Azo dye biodecolorization: An explanatory mechanism 偶氮染料生物脱色过程中粉末活性炭的电化学特性与细菌内部生化过程之间的相互作用：一种解释机制

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-26 DOI: 10.1016/j.procbio.2024.09.025

Drawing on prior reports highlighting the redox mediator properties of powdered activated carbon (PAC), this study was designed to evaluate these properties to enhance the decolorization of azo dye by Klebsiella quasipneumoniae GT7. It was found that the presence of 0.5 % PAC in the medium increased the biodecolorization rate early in incubation. Chemical analysis revealed that dye conversion into aromatic amines occurred in microbial systems both with and without PAC. However, at initial dye concentrations (

C_{id}

) of 2 mM or higher, some dye remained on the PAC surface and in the medium. In contrast, the PAC-free system achieved nearly 100 % biodecolorization at all initial dye concentrations. The negative impact of PAC on decolorization efficiency in microbial systems with high initial dye concentrations cannot be solely explained by its redox mediator function. This study used the amphoteric-Donnan model for PAC's electrical double layer (EDL) and Mitchell's chemiosmotic model for bacterial proton motive force (PMF) to explore this. It found that charge storage in PAC's EDL regulates electron transfer fluxes, and proton species enhance the proton motive force across the bacterial membrane. These observations improve the understanding of PAC's role in microbial decolorization and its potential future applications.

先前的报告强调了粉末活性碳（PAC）的氧化还原介质特性，本研究旨在评估这些特性，以提高准肺炎克雷伯氏菌 GT7 对偶氮染料的脱色能力。研究发现，在培养基中加入 0.5 % 的 PAC 可提高培养初期的生物脱色率。化学分析显示，在有 PAC 和没有 PAC 的微生物系统中，染料都会转化为芳香胺。然而，当初始染料浓度（Cid）为 2 mM 或更高时，一些染料会残留在 PAC 表面和培养基中。相比之下，不含 PAC 的系统在所有初始染料浓度下都几乎实现了 100% 的生物脱色。在初始染料浓度较高的微生物体系中，PAC 对脱色效率的负面影响不能完全由其氧化还原介质功能来解释。本研究利用 PAC 的双电层（EDL）两性-多南模型和 Mitchell 的细菌质子动力（PMF）化学渗透模型对此进行了探讨。研究发现，PAC 双电层中的电荷储存调节着电子传递通量，质子种类增强了细菌膜上的质子动力。这些观察结果加深了人们对 PAC 在微生物脱色过程中的作用及其未来潜在应用的理解。

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引用次数: 0

Antioxidant and anticancer activities on HT-29 colon cancer cells of protein isolate extracted from Cordyceps militaris fruiting body using diverse isolation methods 采用不同分离方法从冬虫夏草子实体中提取的分离蛋白对 HT-29 结肠癌细胞的抗氧化和抗癌活性

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.024

The present research examines different techniques for isolating proteins from Cordyceps militaris fruiting body powder (COR), namely ammonium sulfate precipitation (ASP), organic solvent precipitation (OSP), and isoelectric precipitation (IEP). ASP yielded the highest protein content (78.90 %), effectively concentrating proteins, while OSP and IEP also produced substantial yields (57.41 % and 69.06 %, respectively). Color analysis revealed differences among isolates, with ASP resulting in a darker hue. ASP and IEP showed higher red and yellow components, respectively. Essential amino acid content was significantly higher in ASP isolate compared to COR, indicating effective amino acid concentration. Thermal analysis revealed different decomposition temperatures and enthalpy values among isolates. OSP displayed the highest phenolic content, while IEP exhibited the highest flavonoid content. Antioxidant assays showed ASP to have the highest DPPH-RSA and ABTS-RSA, while IEP showed the highest FRAP value. ASP demonstrated anticancer activity against HT-29 colon cancer cells inducing cell death apoptosis and inhibiting cell migration, with an IC₅₀ of 360.63 µg/mL, comparable to Paclitaxel. These findings suggest that Cordyceps militaris protein isolate, particularly from ASP, has significant antioxidant and anticancer potential, supporting its potential as a natural alternative to synthetic drugs with fewer side effects.

本研究探讨了从冬虫夏草子实体粉（COR）中分离蛋白质的不同技术，即硫酸铵沉淀法（ASP）、有机溶剂沉淀法（OSP）和等电沉淀法（IEP）。硫酸铵沉淀的蛋白质含量最高（78.90%），有效浓缩了蛋白质；有机溶剂沉淀和等电沉淀的蛋白质含量也很可观（分别为 57.41% 和 69.06%）。颜色分析显示了不同分离物之间的差异，ASP 的颜色较深。ASP 和 IEP 的红色和黄色成分分别较高。与 COR 相比，ASP 分离物的必需氨基酸含量明显较高，表明氨基酸浓度较高。热分析表明，不同分离物的分解温度和焓值不同。OSP 的酚含量最高，而 IEP 的类黄酮含量最高。抗氧化测定显示，ASP 的 DPPH-RSA 和 ABTS-RSA 值最高，而 IEP 的 FRAP 值最高。ASP 对 HT-29 结肠癌细胞具有抗癌活性，可诱导细胞凋亡并抑制细胞迁移，其 IC50 值为 360.63 µg/mL，与紫杉醇相当。这些研究结果表明，冬虫夏草蛋白分离物（尤其是 ASP）具有显著的抗氧化和抗癌潜力，支持其作为副作用较少的合成药物天然替代品的潜力。

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引用次数: 0

Pentagalloyl glucose enhanced the stress resistance to delay aging process in Caenorhabditis elegans 五聚酰基葡萄糖增强草履虫的抗应激能力以延缓衰老过程

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.021

Aging is a complex biological process characterized by gradual and irreversible functional deterioration, strongly associated with oxidative stress. Pentagalloyl glucose (PGG) has attracted increasing attention due to its potent antioxidant and anti-stress properties. This study investigated the potential of PGG to mitigate the aging process under stress in RAW 264.7 cells and Caenorhabditis elegans models. The expression of vital genes associated with stress was also measured to explain the action mechanism of PGG in C. elegans. The findings showed that PGG supplementation not only significantly enhanced the stress tolerance of RAW 264.7 cells, but also prolonged lifespan and reduced the ROS and lipofuscin accumulation in C. elegans induced by stress. Meanwhile, the improvement effect of PGG on delaying aging development was also manifested in the protection of mitochondrial function and neuronal integrity. Moreover, daf-16 nuclear translocation and sod-3 expression were significantly enhanced by PGG to delay the aging process. Mechanistically, PGG might alleviate aging by improving daf-16, sod-3, ctl-1, and gst-4 levels in the DAF-16/FOXO pathway and upregulating skn-1 and gst-4 expression in the SKN-1/Nrf2 pathway. Our study provided novel insights into the role of PGG in combating stress-induced aging.

衰老是一个复杂的生物过程，其特点是功能逐渐退化且不可逆转，与氧化应激密切相关。五聚酰基葡萄糖（Pentagalloyl glucose，PGG）因其强大的抗氧化和抗应激特性而受到越来越多的关注。本研究调查了 Pentagalloyl glucose 在 RAW 264.7 细胞和秀丽隐杆线虫模型中缓解压力下衰老过程的潜力。研究还测量了与应激相关的重要基因的表达，以解释 PGG 在秀丽隐杆线虫中的作用机制。研究结果表明，补充 PGG 不仅能显著增强 RAW 264.7 细胞的应激耐受性，还能延长秀丽隐杆线虫的寿命，减少应激诱导的 ROS 和脂褐素积累。同时，PGG 对延缓衰老发展的改善作用还体现在保护线粒体功能和神经元完整性方面。此外，PGG还能显著增强daf-16的核转位和sod-3的表达，从而延缓衰老过程。从机理上讲，PGG可能通过改善DAF-16/FOXO通路中的daf-16、sod-3、ctl-1和gst-4水平以及上调SKN-1/Nrf2通路中的skn-1和gst-4表达来缓解衰老。我们的研究为了解 PGG 在抗应激诱导的衰老中的作用提供了新的见解。

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引用次数: 0

Insight into microorganisms and flavor substances in traditional Chinese fermented food starter: Daqu 洞察中国传统发酵食品开胃菜中的微生物和风味物质：大曲

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.015

Daqu, a crucial fermentation starter in the production of various Chinese fermented foods, plays a pivotal role in shaping complex enzyme profiles and diverse flavour precursors. This review aims to elucidate the microbial communities within Daqu, focusing on their functionalities in the context of flavour development. We delve into the detection methods of microorganisms and flavour substances in Daqu, employing advanced technologies including high-performance liquid chromatography, gas chromatography, pseudo-targeted metabolomics, and headspace solid-phase microextraction-gas chromatography-mass spectrometry. This review explores high throughput sequencing technologies, including pyrosequencing, clonal library sequencing, metaproteomic, and metagenomics, to gain a comprehensive understanding of the intricate microbial dynamics. Additionally, we discuss the metabolic pathways involved in flavour substance production within Daqu. By synthesizing information on Daqu types, microorganisms present, detection methodologies, and flavour substance metabolic pathways, this review contributes to a deeper comprehension of the intricate processes underpinning the Flavors of Chinese fermented foods.

大曲是生产各种中国发酵食品的重要发酵起始菌，在形成复杂的酶谱和多种风味前体方面发挥着关键作用。本综述旨在阐明大曲中的微生物群落，重点关注它们在风味形成过程中的功能。我们深入探讨了大曲中微生物和风味物质的检测方法，采用的先进技术包括高效液相色谱法、气相色谱法、伪靶向代谢组学以及顶空固相微萃取-气相色谱-质谱联用技术。本综述探讨了高通量测序技术，包括热测序、克隆文库测序、元蛋白组学和元基因组学，以全面了解错综复杂的微生物动态。此外，我们还讨论了大曲中香味物质生产所涉及的代谢途径。通过综合大曲类型、存在的微生物、检测方法和风味物质代谢途径等方面的信息，本综述有助于加深对中国发酵食品风味复杂过程的理解。

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引用次数: 0

Understanding Bacillus response to salt stress: Growth inhibition, enhanced EPS secretion, and molecular adaptation mechanisms 了解芽孢杆菌对盐胁迫的反应：生长抑制、EPS 分泌增强和分子适应机制

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-24 DOI: 10.1016/j.procbio.2024.09.023

This study investigates the secretion pattern of extracellular polymeric substances (EPS) by Bacillus sp. under varying salt concentrations and elucidates the molecular mechanisms governing EPS synthesis and secretion. Salt stress inhibited cell proliferation, while optimal salt stimulation promoted EPS secretion, resulting in increased viscosity of the culture medium and the formation of bacterial clusters. Fourier infrared spectrum analysis revealed functional groups such as C-O-C and N-H within the EPS. Soluble-EPS (S-EPS) contained sulfur and phosphorus groups associated with heavy metal ions adsorption. The study also identified a novel polysaccharide formed through bonding EPS (B-EPS). High salt concentrations correlated with elevated levels of tryptophan protein and its derivatives, increased tyrosine polysaccharide derivatives, and decreased aromatic polysaccharides. B-EPS exhibited higher levels of aromatic polysaccharides, with Na⁺ promoting detachment of B-EPS from the cell surface. Transcriptome sequencing (RNA-seq) analysis under salt stress revealed significant expression of spore kinase (KinD) and response regulatory protein Spo0A in the phosphoric acid relay system. Key transcriptional regulatory factors, including OmpR and exopolysaccharide biosynthesis, were closely associated with EPS synthesis and secretion. This study establishes a theoretical foundation for the industrial production and practical application of EPS by elucidating the molecular mechanisms underlying Bacillus' response to salt stress.

本研究探讨了芽孢杆菌在不同盐浓度下分泌胞外聚合物物质（EPS）的模式，并阐明了EPS合成和分泌的分子机制。盐胁迫抑制了细胞增殖，而最佳盐刺激则促进了 EPS 的分泌，导致培养基粘度增加并形成细菌簇。傅立叶红外光谱分析揭示了 EPS 中的 C-O-C 和 N-H 等官能团。可溶性 EPS（S-EPS）含有与重金属离子吸附有关的硫和磷基团。研究还发现了一种通过粘合 EPS（B-EPS）形成的新型多糖。高浓度盐与色氨酸蛋白及其衍生物水平升高、酪氨酸多糖衍生物增加和芳香族多糖减少相关。B-EPS的芳香族多糖含量较高，Na+可促进B-EPS从细胞表面脱离。盐胁迫下的转录组测序（RNA-seq）分析显示，磷酸中继系统中的孢子激酶（KinD）和响应调控蛋白 Spo0A 有显著表达。包括 OmpR 和外多糖生物合成在内的关键转录调控因子与 EPS 的合成和分泌密切相关。本研究通过阐明芽孢杆菌对盐胁迫响应的分子机制，为 EPS 的工业化生产和实际应用奠定了理论基础。

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引用次数: 0

Preparation of a novel laccase-modified Fe3O4/TiO2 catalyst for simultaneous delignification and saccharification of Spartina alterniflora Loisel 制备新型漆酶改性的 Fe3O4/TiO2 催化剂，用于同时进行交替叶斯巴达Loisel的脱木质和糖化处理

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-22 DOI: 10.1016/j.procbio.2024.09.019

The development of a cost-effective and environment-friendly conversion method to overcome the recalcitrance of biomass is a challenging task for enhancing the value-added potential of lignocellulosic compounds. In this study, a photo-enzyme composite catalyst by immobilizing laccase on Fe₃O₄/TiO₂ (denoted as LC-Fe₃O₄/TiO₂) was applied to the pretreatment and enzymatic saccharification of Spartina alterniflora Loisel (SAL) under the action of alternating light/dark cycles. The effect of the pretreatment temperature, pretreatment time, LC-Fe₃O₄/TiO₂ concentration, mass concentration of straw particles, and hydrolysis time on delignification efficiency and reducing sugar yield was investigated by a central composite design (CCD). Under the selected conditions (48 ℃, pretreatment time of 70 min, LC-Fe₃O₄/TiO₂ concentration of 4.9 mg/mL, mass concentration of straw particles of 22 % (w/v), hydrolysis time of 55 h), 92.53 % delignification and 129.85 mg/g reducing sugar yield were achieved. The enzymatic hydrolysis efficiency was higher compared to conventional methods. Moreover, the LC-Fe₃O₄/TiO₂ showed good recyclability and reusability. Developed LC-Fe₃O₄/TiO₂ particles have a high potential for use in biomass utilization.

要提高木质纤维素化合物的增值潜力，开发一种具有成本效益且环境友好的转化方法来克服生物质的难降解性是一项具有挑战性的任务。本研究将固定化漆酶于 Fe3O4/TiO2 上的光酶复合催化剂（简称 LC-Fe3O4/TiO2）应用于光/暗交替循环作用下 Spartina alterniflora Loisel（SAL）的预处理和酶法糖化。通过中心复合设计（CCD）研究了预处理温度、预处理时间、LC-Fe3O4/TiO2浓度、秸秆颗粒质量浓度和水解时间对脱木质素效率和还原糖产量的影响。在所选条件下（48 ℃、预处理时间 70 分钟、LC-Fe3O4/TiO2 浓度 4.9 mg/mL、秸秆颗粒质量浓度 22 % (w/v)、水解时间 55 h），木质素脱除率达到 92.53%，还原糖产量达到 129.85 mg/g。与传统方法相比，酶水解效率更高。此外，LC-Fe3O4/TiO2 还具有良好的可回收性和可再利用性。开发的 LC-Fe3O4/TiO2 颗粒在生物质利用方面具有很大的应用潜力。

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引用次数: 0

Beta-glucan: An overview in biological activities, derivatives, properties, modifications and current advancements in food, health and industrial applications β-葡聚糖：概述生物活性、衍生物、特性、改性以及在食品、健康和工业应用方面的最新进展

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-21 DOI: 10.1016/j.procbio.2024.09.011

β-glucans have garnered significant attention due to their numerous health-promoting and prebiotic attributes. β-glucans, a group of bioactive substance, are known to have biological effects such as immune-modulating, anti-inflammatory, anti-cancer, anti-senescence, hypoglycemic and hypocholesterolemic properties. Owing to its unique physical characteristics including its solubility, viscosity and gelation, the food industry and other sectors have witnessed a rise in the utilization of β-glucan. This study aims to present an overview of β-glucan manufacturing technologies, including extraction, isolation, and purification methods from different sources, comprising of bacteria, fungi and plants such as cereals, with the intention of enhancing these techniques for more productive manufacturing procedures. Additionally, β-glucans' physical characteristics, chemical alterations, prospective industrial uses, and future application prospects in food, pharmaceuticals, cosmetics, and other potentially value-added product are outlined research suggest that β-glucans will become more significant in the global food and health sectors in the future.

β-葡聚糖是一类生物活性物质，具有免疫调节、抗炎、抗癌、抗衰老、降血糖和降胆固醇等生物效应。由于其独特的物理特性，包括溶解性、粘度和凝胶性，β-葡聚糖在食品工业和其他领域的利用率不断上升。本研究旨在介绍β-葡聚糖制造技术的概况，包括从不同来源（包括细菌、真菌和谷物等植物）中提取、分离和纯化的方法，目的是提高这些技术的生产效率。此外，还概述了β-葡聚糖的物理特性、化学变化、预期工业用途以及未来在食品、药品、化妆品和其他潜在增值产品中的应用前景，研究表明，未来β-葡聚糖在全球食品和健康领域将变得更加重要。

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引用次数: 0

Extraction methods of dietary fiber and effect on bioactivity: A review 膳食纤维的提取方法及其对生物活性的影响：综述

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-20 DOI: 10.1016/j.procbio.2024.09.017

A large number of food processing by-products are consumed every day that are not utilized and are rich in highly active nutrients: dietary fiber (DF). DF has been defined by nutritionists as the seventh macronutrient with many benefits to our health, such as improving intestinal flora, reducing obesity and lowering cardiovascular morbidity. Thus, suitable extraction methods are needed to obtain high yields of high and quality DFs (including the soluble dietary fiber and insoluble dietary fiber). DF extraction methods have received increasing attention because it is effective in improving the structure and functional properties of DF. However, to the best of our knowledge, no study has systematically analyzed and summarized the differences in different DF extraction methods and their exert on DF composition, structure and functional activities. In this paper, the extraction methods (physical method, chemical method, biological method and combined method) for DF which have been explored by researchers in the last decade are reviewed, and the effects caused by different extraction methods on the structure and functional activity of DF from different sources are summarized. Moreover, the mechanism, comparative analysis and future trends of different extraction methods for DF are discussed. This review will greatly promote the development and utilization of plant and by-product resources in China, and provide a theoretical basis for the demand of high-quality DFs for our people. Meanwhile, this review can provide theoretical guidance for selecting suitable DF extraction methods and achieving better industrialization of extracted DF.

每天都有大量的食品加工副产品被消耗掉，这些副产品中含有丰富的高活性营养物质：膳食纤维（DF）。膳食纤维被营养学家定义为第七大营养素，对人体健康有诸多益处，如改善肠道菌群、减少肥胖和降低心血管发病率。因此，需要采用合适的提取方法来获得高产量和高质量的 DF（包括可溶性膳食纤维和不可溶性膳食纤维）。膳食纤维提取方法能有效改善膳食纤维的结构和功能特性，因此受到越来越多的关注。然而，据我们所知，目前还没有研究系统地分析和总结不同膳食纤维提取方法的差异及其对膳食纤维成分、结构和功能活性的影响。本文综述了近十年来研究人员探索的 DF 提取方法（物理法、化学法、生物法和组合法），总结了不同提取方法对不同来源 DF 结构和功能活性的影响。此外，还讨论了 DF 不同提取方法的机理、比较分析和未来趋势。该综述将极大地促进我国植物及副产物资源的开发利用，为我国人民对高品质 DF 的需求提供理论依据。同时，本综述还可为选择合适的 DF 提取方法，更好地实现提取 DF 的产业化提供理论指导。

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引用次数: 0

Effects of composite microbial agent with straw or sawdust on organic matter degradation, enzyme activity and functional genes during aerobic composting of food waste 秸秆或锯末复合微生物制剂对厨余垃圾好氧堆肥过程中有机物降解、酶活性和功能基因的影响

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-20 DOI: 10.1016/j.procbio.2024.09.018

Bulking agent and microbial agent are widely used to improving the aerobic composting condition. This study aimed to evaluate the effects of composite microbial agent which is consisted of Azotobacter salinestris, Aspergillus oryzae, Bacillus licheniformis, Bacillus cereus and Trichoderma viride with different bulking agents (straw or sawdust) on organic matter degradation, enzyme activities, and functional genes associated with carbon and nitrogen cycling during aerobic composting of food waste. The results demonstrated that the inoculation of composite microbial agent significantly improved the degradation of starch, protein, cellulose, and grease, along with boosting microbial enzyme activity and the abundance of carbon degradation genes. Furthermore, the degradation of organic matter (OM), protein, cellulose, and grease were higher in the straw bulked composting with composite microbial agent treatment (B). Meanwhile, B also exhibited a higher abundance of cdh and rbcL genes compared to the other treatments. Pearson regression analysis identified temperature as the primary factor controlling organic matter degradation, while cdh and cex genes exhibited strong associations with cellulose degradation during food waste composting. These findings suggest that incorporating straw as bulking agent with composite microbial agent during food waste composting can effectively enhance organic matter degradation.

增容剂和微生物剂被广泛用于改善好氧堆肥条件。本研究旨在评估由盐渍氮核杆菌、黑曲霉、地衣芽孢杆菌、蜡样芽孢杆菌和病毒毛霉组成的复合微生物菌剂与不同的膨松剂（秸秆或锯末）在厨余好氧堆肥过程中对有机物降解、酶活性以及与碳氮循环相关的功能基因的影响。结果表明，接种复合微生物菌剂能显著改善淀粉、蛋白质、纤维素和油脂的降解，同时提高微生物酶活性和碳降解基因的丰度。此外，在使用复合微生物菌剂的秸秆膨化堆肥处理（B）中，有机物（OM）、蛋白质、纤维素和油脂的降解量更高。同时，与其他处理相比，B 处理的 cdh 和 rbcL 基因丰度也更高。皮尔逊回归分析表明，温度是控制有机物降解的主要因素，而 cdh 和 cex 基因则与食物垃圾堆肥过程中的纤维素降解密切相关。这些研究结果表明，在厨余堆肥过程中加入秸秆作为膨松剂和复合微生物菌剂，可以有效促进有机物降解。

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引用次数: 0

Enhanced enzymatic hydrolysis of corn stover by γ-valerolactone pretreatment and the characteristics of enzymatic residues γ-戊内酯预处理对玉米秸秆酶水解的促进作用及酶残留物的特征

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2024-09-19 DOI: 10.1016/j.procbio.2024.09.016

Efficient pretreatment methods are crucial for the generation of fermentable sugars from the renewable lignocellulose. In this study, γ-valerolactone (GVL), a biomass-derived green solvent, was used to fractionate corn stover (CS). The impact of different additives on the enzymatic hydrolysis and pretreatment of CS within the GVL/water system was carefully investigated. The results demonstrate that the use of H₂SO₄-assisted GVL/H₂O (80:20, w/w) pretreatment at mild conditions successfully facilitates the depolymerization of CS, thereby improving the cellulase accessibility. Furthermore, by increasing the GVL pretreatment temperature to 160 °C with 100 mM H₂SO₄ addition, the pretreated CS led to nearly 100 % of hydrolysis yield at 2 % (w/v) solid load. Finally, the thermogravimetric analysis (TGA) was performed to evaluate the thermal decomposition characteristics of untreated CS and enzymatic residues based on the H₂SO₄-assisted GVL/H₂O pretreatment. The results indicate that the enzymatic residues had two depolymerization stages from 200 °C to 500 °C with a final residual mass of 22.73 %, which was higher than that of untreated CS (20.56 %). Overall, this study provides an efficient lignocellulose pretreatment for fermentable sugars production and valorization of enzymatic residues.

高效的预处理方法对于从可再生木质纤维素中生成可发酵糖类至关重要。在本研究中，γ-戊内酯（GVL）作为一种生物质衍生的绿色溶剂，被用于分馏玉米秸秆（CS）。研究仔细考察了不同添加剂对 GVL/水体系中玉米秸秆酶水解和预处理的影响。结果表明，在温和条件下使用 H2SO4 辅助 GVL/H2O（80:20，w/w）预处理成功地促进了 CS 的解聚，从而提高了纤维素酶的可及性。此外，通过将 GVL 预处理温度升高到 160 °C，并添加 100 mM H2SO4，预处理后的 CS 在固含量为 2% (w/v) 时的水解率接近 100%。最后，热重分析（TGA）评估了未经处理的 CS 和基于 H2SO4 辅助 GVL/H2O 预处理的酶残留物的热分解特性。结果表明，酶解残渣在 200 °C 至 500 °C 之间有两个解聚阶段，最终残留质量为 22.73%，高于未经处理的 CS（20.56%）。总之，本研究提供了一种高效的木质纤维素预处理方法，可用于发酵糖的生产和酶解残留物的增值。

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引用次数: 0