Process Biochemistry最新文献

Heterologous N-glycosyltransferase expression reprograms the glycoproteome and functional profile of Bacillus subtilis WB800N 异源n -糖基转移酶的表达对枯草芽孢杆菌WB800N的糖蛋白组和功能谱进行了重编程

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-14 DOI: 10.1016/j.procbio.2025.12.008

Zhaoxi Liu, Yue Yu, Yupin Nie, Jiangyu Yang, Min Chen

Engineering bacterial glycosylation represents a promising strategy to modulate protein function and cellular physiology. In this study, we introduced a heterologous N-glycosyltransferase (AaNGT) into Bacillus subtilis WB800N to explore its impact on the host glycoproteome and functional profile. The recombinant strain successfully expressed and purified AaNGT, which exhibited in vitro activity toward UDP-Gal and UDP-Glc, as confirmed by TLC and HPLC analyses. Comparative glycoproteomic profiling revealed that AaNGT expression substantially increased N-glycosylation events, diversifying glycoprotein targets across metabolic enzymes, stress response proteins, and structural components. Functional annotation indicated enrichment in amino acid metabolism, secondary metabolite biosynthesis, stress and detoxification pathways, and signal transduction processes, suggesting that protein glycosylation remodels host metabolic and regulatory networks. Moreover, engineered glycosylation enhanced adhesion to Caco-2 cells and improved tolerance to simulated gastric and intestinal conditions, highlighting potential benefits for strain stability and survivability. Together, these findings demonstrate that heterologous NGT-mediated glycosylation can reprogram the glycoproteome and functional landscape of B. subtilis, providing a versatile platform for engineering Gram-positive chassis strains with tailored metabolic and physiological traits.

工程细菌糖基化代表了一种有前途的策略来调节蛋白质功能和细胞生理。本研究将异源n -糖基转移酶（AaNGT）导入枯草芽孢杆菌WB800N，探讨其对宿主糖蛋白组和功能谱的影响。重组菌株成功表达并纯化了AaNGT，经薄层色谱和高效液相色谱分析证实，AaNGT对UDP-Gal和UDP-Glc具有体外活性。比较糖蛋白组学分析显示，AaNGT表达显著增加了n -糖基化事件，使糖蛋白靶点在代谢酶、应激反应蛋白和结构组分中多样化。功能注释表明，在氨基酸代谢、次生代谢物生物合成、应激和解毒途径以及信号转导过程中富集，表明蛋白质糖基化重塑了宿主的代谢和调节网络。此外，工程糖基化增强了Caco-2细胞的粘附，提高了对模拟胃和肠道条件的耐受性，突出了菌株稳定性和生存能力的潜在益处。总之，这些发现表明，异源ngt介导的糖基化可以重新编程枯草芽孢杆菌的糖蛋白组和功能景观，为具有定制代谢和生理特性的革兰氏阳性底盘菌株的工程提供了一个通用平台。

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引用次数: 0

Laccase-mediated oxidative polymerization of gallic acid for wool fabric dyeing and functionalization 漆酶介导的没食子酸氧化聚合对羊毛织物的染色及功能化

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-14 DOI: 10.1016/j.procbio.2025.12.010

Xue Han , Dong Han , Aixue Dong , Jinghao Cai , Jing Shen , Runbo Wang

Laccase-catalyzed gallic acid polymerization represents a clean, in-situ dyeing method for wool fabrics that addresses the pollution issues in traditional dyeing processes. This study employs four dyeing processes to achieve laccase-catalyzed polymerization of gallic acid on wool fabrics, systematically comparing the effects of the different processes on dyeing performance and functional properties. The results demonstrate that the pre-polymerization and subsequent dyeing method produced the deepest color (K/S = 5.33) and the highest level of UV protection (UPF = 306.32) of all the processes. All dyed fabrics exhibited good color fastness (≥ grade 3) and enhanced functionality, including improved tensile strength and increased hydrophilicity, albeit with reduced alkali resistance. Spectroscopic analysis confirms the formation of covalent bonds between wool fibers and polymerized gallic acid via laccase-mediated oxidation. These findings highlight advancements in the enzymatic polymerization of phenolic compounds for colored textile surfaces and offer potential applications in enzymatic dyeing and sustainable textile processing.

漆酶催化没食子酸聚合代表了一种清洁的羊毛织物原位染色方法，解决了传统染色工艺中的污染问题。本研究采用四种染色工艺在羊毛织物上实现漆酶催化没食子酸聚合，系统比较不同染色工艺对羊毛织物染色性能和功能性能的影响。结果表明，预聚合后染色法的颜色最深（K/S = 5.33），紫外线防护水平最高（UPF = 306.32）。所有染色织物都表现出良好的色牢度（≥3级）和增强的功能性，包括提高抗拉强度和亲水性，尽管耐碱性降低。光谱分析证实羊毛纤维和聚合没食子酸之间通过漆酶介导的氧化形成共价键。这些发现突出了酶促聚合酚类化合物用于有色纺织品表面的进展，并在酶促染色和可持续纺织品加工中提供了潜在的应用。

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引用次数: 0

Gradient voltages stimulation of biogenic coalbed methane: Insights into the evolution of distinct habitats 梯度电压对生物成因煤层气的刺激作用：对不同生境演化的洞察

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-14 DOI: 10.1016/j.procbio.2025.12.011

Yifan Yin , Zhicheng Shi , Jiayan Zhang , Xiao Feng , Hongguang Guo , Zaixing Huang , Michael Urynowicz

Microbial electrolysis cells (MECs) could enhance anaerobic biodegradation of coal, thereby facilitating biogenic methane production. This study investigated the effects of gradient voltages (0 V-1.5 V) on coal-to-methane conversion. The changes in methane production, current, microbial communities, coal structure and liquid-phase organics were analyzed. Methane production peaked at 330.63 µmol/g coal at 1.2 V, increased by 31.4 % compared with 0 V. Effective voltage generated current peaks in early stage, facilitating organics oxidation. High-throughput sequencing and quantitative PCR revealed that Pseudomonas and Enterobacter were dominant bacteria in sediment, suspension, anode, and the maximum bacterial biomass in sediment and suspension increased by 66.1 % and 47.4 %, respectively. Methanosarcina and Bathyarchaeia in suspension were main archaea for methane production, with total biomass increasing by 46.8 % at 0.6 V and 83.4 % at 1.2 V. FTIR and GC-MS results confirmed that voltage stimulation accelerated coal biodegradation, utilized aromatic substituents and oxygen-containing groups, increased aliphatic branching and enhanced transformation of hydrocarbons. Overall, applied voltages could enhance methane production, with 0.6 V and 1.2 V showing more pronounced effects. This was achieved by providing electrons, increasing biomass, and promoting degradation. The findings offered theoretical guidance for the application of MECs to enhance coal-to-methane conversion.

微生物电解细胞（MECs）可以促进煤的厌氧生物降解，从而促进生物甲烷的生产。本研究考察了梯度电压（0 V-1.5 V）对煤制甲烷转化的影响。分析了甲烷产量、电流、微生物群落、煤结构和液相有机物的变化。甲烷产量在1.2 V时达到峰值，为330.63µmol/g煤，比0 V时增加了31.4% %。有效电压产生的电流在早期达到峰值，有利于有机物氧化。高通量测序和定量PCR结果显示，沉积物、悬浮液和阳极中的优势菌为假单胞菌和肠杆菌，沉积物和悬浮液中的最大细菌生物量分别增加了66.1% %和47.4% %。悬浮菌中产甲烷的主要古菌为甲烷生菌（Methanosarcina）和深海生菌（Bathyarchaeia），在0.6 V和1.2 V下总生物量分别增加46.8 %和83.4 %。FTIR和GC-MS结果证实，电压刺激加速了煤的生物降解，利用了芳香族取代基和含氧基团，增加了脂肪族分支，促进了碳氢化合物的转化。总的来说，施加电压可以提高甲烷产量，0.6 V和1.2 V的效果更明显。这是通过提供电子、增加生物量和促进降解来实现的。研究结果为mec在提高煤制甲烷转化率方面的应用提供了理论指导。

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引用次数: 0

Investigation of technological, chemical, biological aspects, and in-silico pharmacokinetic and docking predictions of winter savory (Satureja montana L.) essential oil 技术，化学，生物方面的调查，以及冬季风味（Satureja montana L.）精油的计算机药代动力学和对接预测

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-13 DOI: 10.1016/j.procbio.2025.12.009

Snežana Filip , Saša D. Đurović , Slavica Blagojević , Olivera Delić , Angi E. Skhvediani , Andrey V. Vasin , Darko M. Micić

Hydrodistillation (HD 205 W and 410 W) and microwave-assisted hydrodistillation (MWHD 90–800 W) were used to prepare essential oil samples from winter savory. Isolated essential oils were analyzed to determine their chemical profiles. Carvacrol was the principal compound, followed by p-cymene and γ-terpinene. Biological activity was assessed using six antioxidant assays, an antimicrobial assay (6 different microbes), and a cytotoxic test (4 cell lines). The MWHD 360 W was the most potent antioxidant agent. The HD 205 W and MWHD 360 W showed the best antimicrobial activity, while the HD essential oil samples were the most potent cytotoxic agents. Molecular docking was performed for the eight most abundant compounds on three proteins: E. coli type I signal peptidase (PDB code 1T7D), P. aeruginosa arginine rhamosyltransferase (PDB code 6J7J), and S. aureus penicillin G acyl-Penicillin binding protein 2a (PDB code 1MWT). Carvacryl acetate and trans-caryophyllene provide the most promising results (the lowest binding energy). The ADMET (absorption, distribution, metabolism, excretion, and toxicity) calculations revealed low toxicity, high skin, blood-brain barrier, and CNS permeability, and high intestinal absorption.

采用加氢蒸馏法（hd205 W和410 W）和微波辅助加氢蒸馏法（MWHD 90-800 W）制备冬香菜精油样品。对分离的精油进行分析以确定其化学成分。香芹酚是主要化合物，其次是对花香烃和γ-萜烯。采用六种抗氧化试验、一种抗微生物试验（6种不同的微生物）和一种细胞毒性试验（4种细胞系）评估生物活性。MWHD 360 W是最有效的抗氧化剂。HD 205 W和MWHD 360 W的抑菌活性最好，而HD精油样品的细胞毒性最强。对大肠杆菌I型信号肽酶（PDB代码1T7D）、铜绿假单胞菌精氨酸糖基转移酶（PDB代码6J7J）和金黄色葡萄球菌青霉素G酰基-青霉素结合蛋白2a （PDB代码1MWT）这3种蛋白上最丰富的8个化合物进行了分子对接。醋酸石竹酯和反式石竹烯提供了最有希望的结果（最低的结合能）。ADMET（吸收、分布、代谢、排泄和毒性）计算显示毒性低，皮肤、血脑屏障和中枢神经系统通透性高，肠道吸收高。

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引用次数: 0

SoluBat: A bidirectional mamba framework for high-throughput protein solubility prediction in bioprocess optimization SoluBat：用于生物工艺优化中高通量蛋白质溶解度预测的双向曼巴框架

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-11 DOI: 10.1016/j.procbio.2025.12.006

Linjie Wu , Chen Zhang , Zijian Hu , Xurong Wang , Hongrong Liu , Bin Zhu

Protein solubility prediction is a critical step in protein engineering and biomanufacturing, directly influencing wet-lab pre-screening costs and the efficiency of high-throughput R&D. To address the limitations of existing methods in computational efficiency and model interpretability, this study proposes SoluBat, a multimodal deep learning model based on a bidirectional Mamba architecture. SoluBat integrates protein sequence, evolutionary information (PSSM), and physicochemical properties, dynamically fusing these features via an efficient gating mechanism to achieve a balance between performance and energy consumption. Experimental results demonstrate that SoluBat achieves higher accuracy and Matthews correlation coefficient than mainstream deep learning and traditional methods on multiple public datasets, while markedly reducing computational resource consumption. In addition, the model is equipped with built-in per-residue attribution analysis, supporting rational protein design. SoluBat thus provides an efficient and interpretable computational solution for high-throughput protein solubility prediction and industrial applications.

蛋白质溶解度预测是蛋白质工程和生物制造的关键步骤，直接影响湿实验室预筛选成本和高通量研发效率。为了解决现有方法在计算效率和模型可解释性方面的局限性，本研究提出了基于双向Mamba架构的多模态深度学习模型SoluBat。SoluBat集成了蛋白质序列、进化信息（PSSM）和物理化学性质，通过有效的门控机制动态融合这些特征，以实现性能和能耗之间的平衡。实验结果表明，在多个公共数据集上，与主流深度学习和传统方法相比，SoluBat获得了更高的准确率和马修斯相关系数，同时显著降低了计算资源消耗。此外，该模型还内置了每残基属性分析，支持合理的蛋白质设计。因此，SoluBat为高通量蛋白质溶解度预测和工业应用提供了高效和可解释的计算解决方案。

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引用次数: 0

Beyond conductivity: Specific surface area and surface functional groups as determinants for chain elongation on carbon materials 超越电导率：比表面积和表面官能团作为碳材料链延伸的决定因素

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-10 DOI: 10.1016/j.procbio.2025.12.007

Yuhao Liu , Lei Zhao , Haiyang Lv , Xinyi Wang , Long Chen , Haihua Li , Ruihua Li , Xin Du , Shuli Liu , Haiping Gu

In this study, bamboo charcoal, coconut shell charcoal, flake graphite powder, and graphene were used as mediators to investigate the roles of electrical conductivity, specific surface area (SSA), and surface functional groups in the chain elongation (CE) for medium-chain fatty acid (MCFAs) synthesis. The study compared biomass charcoals (bamboo charcoal and coconut shell charcoal) and graphitic materials (flake graphite powder and graphene), controlling their total SSA at 1800 m²/L and 100 m²/L, respectively. Both bamboo and coconut shell charcoal significantly promoted MCFAs synthesis, with the latter showing superior performance, likely due to its higher conductivity and richer functional groups. In contrast, graphitic materials, despite their much higher conductivity, showed limited to no promotion of MCFAs production; flake graphite powder mainly enhanced butyrate synthesis. These results demonstrate that for different types of carbon materials, high conductivity does not necessarily lead to an increase in CE. The key functional microorganism, Clostridium_sensu_stricto_12, showed significant correlation with the yield, selectivity, and electron transfer efficiency of MCFAs, and its abundance also showed a notable correlation with the SSA and surface functional group content of the carbon materials, but not their conductivity. Metagenomic analysis revealed that in the CE system, both the reverse β-oxidation (RBO) pathway and the fatty acid biosynthesis (FAB) pathway are present, with the RBO pathway being the dominant pathway.

本研究以竹炭、椰子壳炭、片状石墨粉和石墨烯为介质，研究了导电率、比表面积（SSA）和表面官能团对中链脂肪酸（MCFAs）合成链伸长（CE）的影响。研究将生物质炭（竹炭和椰壳炭）和石墨材料（片状石墨粉和石墨烯）的总SSA分别控制在1800 m2/L和100 m2/L。竹炭和椰壳炭均能显著促进MCFAs的合成，其中椰壳炭表现出更优异的性能，可能是由于其导电率更高、官能团更丰富。相比之下，石墨材料尽管具有更高的导电性，但对MCFAs生产的促进作用有限或没有；片状石墨粉主要增强了丁酸盐的合成。这些结果表明，对于不同类型的碳材料，高导电性并不一定导致CE的增加。关键功能微生物Clostridium_sensu_stricto_12与MCFAs的产率、选择性和电子传递效率呈显著相关，其丰度与碳材料的SSA和表面官能团含量呈显著相关，但与其电导率无显著相关。宏基因组分析显示，在CE系统中，存在反向β-氧化（RBO）途径和脂肪酸生物合成（FAB）途径，其中RBO途径是主要途径。

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引用次数: 0

Enhanced adsorption capacity of mesoporous composite agarose beads for glycoprotein separation: Experiments and mathematical modeling as a prerequisite for scale-up 用于糖蛋白分离的介孔复合琼脂糖珠的增强吸附能力：实验和数学建模作为扩大规模的先决条件

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-10 DOI: 10.1016/j.procbio.2025.12.005

Aref Honarparvaran , Mohammad Reza Mehrnia , Fatemeh Pourasgharian Roudsari

Affinity chromatography is an economic bottleneck in scaling downstream bioprocesses. This study employs experimental and modeling methodologies to examine key parameters influencing the adsorption capacity of a medium-sized glycoprotein on boronate affinity column. To investigate the impact of intraparticle nanostructure on adsorption capacity, Composite Beads were fabricated, exhibiting enhanced porosity (30 %) and mechanical robustness (18 %). The increased dynamic binding capacity (DBC) of columns suggests facilitated pore diffusion within Composite Beads’ mesoporous structure. Mathematical modeling using the General Rate Model (GRM) and the Pore Diffusion Model (PDM) was utilized to predict the columns’ breakthrough curves and the beads’ saturation rate, respectively. PDM revealed a relatively prolonged saturation of Composite Beads compared to Agarose Beads. Evaluating the influence of column dimensions on the DBCs elucidated that enlarging the column diameter wielded a more pronounced impact (15 %) than altering the column length (9 %), highlighting the importance of overcoming intraparticle diffusion resistance. The non-equilibrium-based GRM, not only predicted breakthrough curves of columns with varying dimensions (Absolute Average Deviation of 7.63 %) but also the intricate behavior of columns packed with Beads possessing diverse adsorption capacities. The outcomes of this study provided valuable insights for the prospective scale-up of nanostructured beads in affinity chromatography columns.

亲和色谱法是扩大下游生物工艺规模的经济瓶颈。本研究采用实验和建模方法，研究了影响中型糖蛋白在硼酸盐亲和柱上吸附能力的关键参数。为了研究颗粒内纳米结构对吸附能力的影响，制备了具有增强孔隙率（30 %）和机械鲁棒性（18 %）的复合微珠。柱的动态结合能（DBC）的增加表明复合微珠介孔结构内的孔隙扩散更容易。采用通用速率模型（General Rate Model， GRM）和孔隙扩散模型（Pore Diffusion Model， PDM）进行数学建模，分别预测了柱的突破曲线和球的饱和速率。与琼脂糖珠相比，PDM显示复合珠的饱和时间相对较长。评估柱尺寸对DBCs的影响表明，扩大柱直径比改变柱长（9 %）具有更显著的影响（15 %），突出了克服颗粒内扩散阻力的重要性。基于非平衡的GRM不仅预测了不同尺寸柱的突破曲线（绝对平均偏差为7.63 %），而且还预测了具有不同吸附容量的小球填充柱的复杂行为。本研究的结果为亲和色谱柱中纳米结构珠的预期扩大提供了有价值的见解。

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引用次数: 0

Corrigendum to “Z. officinale-doped silver/calcium oxide nanocomposites: Catalytic activity and antimicrobial potential with molecular docking analysis” [Process Biochem. 121 (2022) 635–646] Z的勘误表。掺杂银/氧化钙纳米复合材料：催化活性和抗菌潜力的分子对接分析[j] .生物化学学报，21(2022):635-646。

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-08 DOI: 10.1016/j.procbio.2025.12.003

Zakariya Mehmood , Muhammad Ikram , Muhammad Imran , Anum Shahzadi , Ali Haider , Anwar Ul-Hamid , Walid Nabgan , Junaid Haider , Shaukat Hayat

引用次数: 0

Composition analysis and prediction of sulfuric acid-assisted steam-exploded corn stover slurry: Guiding parameters selection 硫酸辅助蒸汽爆破玉米秸秆浆料成分分析与预测：指导参数选择

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-06 DOI: 10.1016/j.procbio.2025.12.004

Yinghui He, Hui Zhang, Kaili Ding, Xueyan Liang, Xiangjie Zuo, Nan Zhang, Li Liu, Suan Shi, Lujia Han, Weihua Xiao

The slurry compositions of sulfuric acid-assisted steam explosion (SASE) corn stover are strongly dependent on process parameters. Preliminary assessment of these compositions facilitates the rapid selection of SASE parameters. In this study, curve fitting and support vector regression (SVR) were employed to elucidate the variation trends of components in SASE slurry and achieve prediction. Curve fittings clarified the variation patterns of components with the combined severity factor (CSF), exhibiting high goodness-of-fit (R² > 0.942 and RMSE < 0.287), and were utilized to identify optimal CSF balancing inhibitors, cellulose, and xylooligosaccharides (XOS). The co-production of 8.20 g/L XOS and 40.48 g/L enzymatic hydrolysis sugars was achieved. Additionally, the SVR model realized the multivariate nonlinear fitting of pressure, time, and acid concentration, and analyzed the contribution degree of operating parameters, thereby providing more robust guidance for parameter selection. The SVR model demonstrated exceptional predictive performance, achieving R² > 0.969 and RMSE < 0^.436. The variation between experimental values and predicted values was less than 20 %. In conclusion, the integrated framework of curve fitting and SVR offers a reliable, rapid approach that not only simplifies the parameter optimization process of SASE pretreatment but also provides a practical basis for industrial application.

硫酸辅助蒸汽爆破（SASE）玉米秸秆料浆的组成与工艺参数有很大的关系。对这些成分的初步评价有助于快速选择SASE参数。本研究采用曲线拟合和支持向量回归（SVR）来阐明SASE浆料中各组分的变化趋势并进行预测。曲线拟合明确了成分与联合严重程度因子（CSF）的变化模式，具有较高的拟合优度（R2 > 0.942, RMSE < 0.287），并用于确定最佳的CSF平衡抑制剂，纤维素和低聚木糖（XOS）。共产XOS 8.20 g/L和酶解糖40.48 g/L。此外，SVR模型实现了压力、时间、酸浓度的多元非线性拟合，分析了运行参数的贡献程度，为参数选择提供了更稳健的指导。SVR模型表现出优异的预测性能，R2 >； 0.969,RMSE <； 0.436。实验值与预测值的偏差小于20% %。综上所述，曲线拟合和支持向量回归的集成框架提供了一种可靠、快速的方法，不仅简化了SASE预处理的参数优化过程，而且为工业应用提供了实践基础。

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引用次数: 0

Economic and technical insights into carotenoids and lipids large-scale production in Rhodotorula glutinis: A study based on pulse-feeding culture, cell disruption strategies and cytotoxicity 粘红酵母类胡萝卜素和脂类大规模生产的经济和技术见解：一项基于脉冲饲养培养、细胞破坏策略和细胞毒性的研究

IF 4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry

Pub Date : 2025-12-04 DOI: 10.1016/j.procbio.2025.12.002

Carolina Ramírez-Martínez , Susana Rincón , Alejandro Zepeda , Alberto Ordaz

The oleaginous yeast Rhodotorula glutinis is a promising microorganism for simultaneous production of carotenoids and lipids which are traditionally derived from plants and chemical synthesis; however, their production faces challenges such as stability and health issues, supply consistency, and large-scale production studies. This study focused on the use of Rhodotorula glutinis with a pulse-feeding culture strategy in a stirred tank reactor to intensify the biomass concentration (52.84 ± 0.5 g/L). Effective cell disruption for products extraction was achieved using acid treatment and cold solvent extraction, yielding 3.21 ± 0.5 mg/g dry yeast carotenoids and 0.175 ± 0.01 g/g dry yeast lipids. A safety test on human fibroblasts showed no cytotoxic effects in the obtained extract. Using experimental results as input parameters, three simulation scenarios were constructed in SuperPro Designer, with a product composed of lipids (97.41 % w/w) and carotenoids (2.59 % w/w): Scenario 1 (500 kg/year), Scenario 2 (1000 kg/year), and Scenario 3 (2000 kg/year). Adequate selection of equipment size can reduce both operational and capital expenses. Revenues were mainly linked to lipid production due to higher mass yields. Future work should focus on process scalability, feeding regime optimization, medium formulation, extract composition and functionality, and environmental impact assessment.

产油酵母粘红酵母（Rhodotorula glutinis）是一种很有前途的微生物，可以同时生产类胡萝卜素和脂类，这两种物质传统上是从植物和化学合成中获得的；然而，它们的生产面临着稳定性和健康问题、供应一致性和大规模生产研究等挑战。本研究主要研究了在搅拌槽式反应器中采用脉冲投料培养方法提高粘红酵母的生物量浓度（52.84 ± 0.5 g/L）。通过酸处理和冷溶剂萃取实现了有效的细胞破坏，得到3.21 ± 0.5 mg/g干酵母类胡萝卜素和0.175 ± 0.01 g/g干酵母脂质。对人成纤维细胞的安全性试验表明，所获得的提取物没有细胞毒性作用。以实验结果为输入参数，在SuperPro Designer中构建了三种模拟场景，其中产品由脂质（97.41 % w/w）和类胡萝卜素（2.59 % w/w）组成：场景1（500 kg/年）、场景2（1000 kg/年）和场景3（2000 kg/年）。适当选择设备尺寸可以减少运营和资本支出。由于产量较高，收入主要与油脂生产有关。未来的工作应集中在工艺可扩展性、投料制度优化、培养基配方、提取物组成和功能以及环境影响评价等方面。

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引用次数: 0