Pub Date : 2024-11-14DOI: 10.1016/j.procbio.2024.11.015
Nasrin Sultana , Riya Pathak , Shibnath Samanta , Neelotpal Sen Sarma
Photothermal and photodynamic therapies use light to target and eliminate cancer cells by raising the temperature or producing reactive oxygen species. To understand the main effect of both the therapy, studies are going on at the cellular level. Based on the distinct mode of action both photodynamic and photothermal therapy may work synergically according to recent studies. As both therapies are non-toxic, the combination of these two may be more effective for curing cancer. Additionally, a combination of the two therapies is being used to develop nano-platforms that incorporate simultaneously photothermally and photodynamically active drugs. In this review, we have discussed the basic mechanism of both the therapy and different mechanisms such as cell death mechanism, vascular damage, extracellular matrix effect as well as immune effect. Along with that, we have also discussed different factors that can affect photodynamic and photothermal therapy. Additionally, we have also discussed the different types of nanomaterials that can be useful for photodynamic therapy as well as in photothermal therapy.
{"title":"A comprehensive analysis of photothermal therapy (PTT) and photodynamic therapy (PDT) for the treatment of cancer","authors":"Nasrin Sultana , Riya Pathak , Shibnath Samanta , Neelotpal Sen Sarma","doi":"10.1016/j.procbio.2024.11.015","DOIUrl":"10.1016/j.procbio.2024.11.015","url":null,"abstract":"<div><div>Photothermal and photodynamic therapies use light to target and eliminate cancer cells by raising the temperature or producing reactive oxygen species. To understand the main effect of both the therapy, studies are going on at the cellular level. Based on the distinct mode of action both photodynamic and photothermal therapy may work synergically according to recent studies. As both therapies are non-toxic, the combination of these two may be more effective for curing cancer. Additionally, a combination of the two therapies is being used to develop nano-platforms that incorporate simultaneously photothermally and photodynamically active drugs. In this review, we have discussed the basic mechanism of both the therapy and different mechanisms such as cell death mechanism, vascular damage, extracellular matrix effect as well as immune effect. Along with that, we have also discussed different factors that can affect photodynamic and photothermal therapy. Additionally, we have also discussed the different types of nanomaterials that can be useful for photodynamic therapy as well as in photothermal therapy.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 17-31"},"PeriodicalIF":3.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1016/j.procbio.2024.11.008
Andri Frediansyah
Soybean processing has traditionally relied on fermentation. These fermented products have been consumed for decades, particularly in Asian countries. Despite numerous publications on fermented soybeans over the past ninety years, there is a lack of reviews that specifically focus on nine traditional fermented soybean products, namely tempeh, natto, doenjang, chungkookjang, douchi, meju, kinema, oncom, and tauco. Population growth and the need for a healthy diet have prompted researchers to improve soybean-fermented products. The objective of this study was to use reference data to conduct a bibliometric analysis assessing the progress of research on nine traditional fermented soybean products, various uses of these products, and the utilization of advanced technologies to improve research quality. From the scientific database, 2280 documents published between 1928 and 2024 were identified and subjected to bibliometric analysis using the VOSviewer software. Up until recently, article publications and citations increased. South Korea holds the top position in the quantity of publications related to the NTFSP. IPB University is the most productive institution regarding the nine traditional fermented soybean products. In vivo and clinical trials have demonstrated that certain traditional fermented products possess anti-metabolic syndrome properties, including antidiabetic, anti-obesity, antihypertensive effects, cancer, and modulation of bone metabolism. Microbial diversity also provides health benefits to fermented soy products. Furthermore, recent trends include leveraging emerging techniques such as omics, docking, and whole-genome sequencing to advance research on fermented soybeans. However, no references were found for either the in vivo or clinical trials of douchi, meju, kinema, or oncom. Moreover, the use of recent advancement tools has not yet been found in literature related to oncom and tauco. Therefore, using advanced tools, further research on combating metabolic syndrome-related fermented soybean products should be carried out.
{"title":"Growth in traditional fermented soybeans-related research (tempeh, natto, doenjang, chungkookjang, douchi, meju, kinema, oncom, and tauco) from 1928 to 2024, following the omics emergence and trend in functional food","authors":"Andri Frediansyah","doi":"10.1016/j.procbio.2024.11.008","DOIUrl":"10.1016/j.procbio.2024.11.008","url":null,"abstract":"<div><div>Soybean processing has traditionally relied on fermentation. These fermented products have been consumed for decades, particularly in Asian countries. Despite numerous publications on fermented soybeans over the past ninety years, there is a lack of reviews that specifically focus on nine traditional fermented soybean products, namely tempeh, natto, doenjang, chungkookjang, douchi, meju, kinema, oncom, and tauco. Population growth and the need for a healthy diet have prompted researchers to improve soybean-fermented products. The objective of this study was to use reference data to conduct a bibliometric analysis assessing the progress of research on nine traditional fermented soybean products, various uses of these products, and the utilization of advanced technologies to improve research quality. From the scientific database, 2280 documents published between 1928 and 2024 were identified and subjected to bibliometric analysis using the VOSviewer software. Up until recently, article publications and citations increased. South Korea holds the top position in the quantity of publications related to the NTFSP. IPB University is the most productive institution regarding the nine traditional fermented soybean products. <em>In vivo</em> and clinical trials have demonstrated that certain traditional fermented products possess anti-metabolic syndrome properties, including antidiabetic, anti-obesity, antihypertensive effects, cancer, and modulation of bone metabolism. Microbial diversity also provides health benefits to fermented soy products. Furthermore, recent trends include leveraging emerging techniques such as omics, docking, and whole-genome sequencing to advance research on fermented soybeans. However, no references were found for either the <em>in vivo</em> or clinical trials of douchi, meju, kinema, or oncom. Moreover, the use of recent advancement tools has not yet been found in literature related to oncom and tauco. Therefore, using advanced tools, further research on combating metabolic syndrome-related fermented soybean products should be carried out.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 600-624"},"PeriodicalIF":3.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1016/j.procbio.2024.11.010
Eun-Ji Lee , Hoon-Min Lee , Hyun-Seung Kim , So Hui Ryu , Mi-Jung Kang , Jungmok You , Yeon-Gu Kim
Protein fragmentation is a critical quality attribute for Fc-fusion protein production in mammalian cells. In the production of viral non-structural proteins as the form of Fc-fusion protein, fragmentation of Fc-fusion proteins occurred in two transient gene expression (TGE) systems with human embryonic kidney (HEK) 293 and Chinese hamster ovary (CHO) cells. The introduction of a flexible empirical linker reduced fragmentation in HEK293 cells, but not in CHO cells. Additionally, two rigid empirical linkers failed to restore impaired Fc-fusion proteins in CHO cells. In vitro incubation assay using conditioned culture medium and cultures supplemented with protease inhibitor cocktail suggest that fragmentation of Fc-fusion proteins in CHO cells may be due to various host cell proteins released into the culture medium. These findings suggest that the introduction of linker peptides can improve the fragmentation of Fc-fusion proteins in mammalian cells, but exhibit different fragment patterns depending on the cell type.
蛋白质破碎是哺乳动物细胞生产 Fc 融合蛋白的一个关键质量属性。在以 Fc 融合蛋白形式生产病毒非结构蛋白的过程中，Fc 融合蛋白在人胚胎肾（HEK）293 和中国仓鼠卵巢（CHO）细胞的两个瞬时基因表达（TGE）系统中发生了破碎。在 HEK293 细胞中，引入一个柔性经验连接子可减少碎裂，但在 CHO 细胞中却不能。此外，两个刚性经验连接体也无法恢复 CHO 细胞中受损的 Fc 融合蛋白。使用条件培养液和添加蛋白酶抑制剂鸡尾酒的培养液进行的体外培养试验表明，CHO 细胞中 Fc 融合蛋白的破碎可能是由于各种宿主细胞蛋白释放到培养液中造成的。这些研究结果表明，引入连接肽可改善哺乳动物细胞中 Fc 融合蛋白的破碎，但不同的细胞类型会表现出不同的破碎模式。
{"title":"Investigating the effect of linker peptides on the fragmentation of Fc-fusion proteins in transient gene expression of mammalian cells","authors":"Eun-Ji Lee , Hoon-Min Lee , Hyun-Seung Kim , So Hui Ryu , Mi-Jung Kang , Jungmok You , Yeon-Gu Kim","doi":"10.1016/j.procbio.2024.11.010","DOIUrl":"10.1016/j.procbio.2024.11.010","url":null,"abstract":"<div><div>Protein fragmentation is a critical quality attribute for Fc-fusion protein production in mammalian cells. In the production of viral non-structural proteins as the form of Fc-fusion protein, fragmentation of Fc-fusion proteins occurred in two transient gene expression (TGE) systems with human embryonic kidney (HEK) 293 and Chinese hamster ovary (CHO) cells. The introduction of a flexible empirical linker reduced fragmentation in HEK293 cells, but not in CHO cells. Additionally, two rigid empirical linkers failed to restore impaired Fc-fusion proteins in CHO cells. <em>In vitro</em> incubation assay using conditioned culture medium and cultures supplemented with protease inhibitor cocktail suggest that fragmentation of Fc-fusion proteins in CHO cells may be due to various host cell proteins released into the culture medium. These findings suggest that the introduction of linker peptides can improve the fragmentation of Fc-fusion proteins in mammalian cells, but exhibit different fragment patterns depending on the cell type.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 625-629"},"PeriodicalIF":3.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Here we report the preparation and characterization of novel enzyme supports based on silica-coated Fe3O4 magnetic nanoparticles. These nanomaterials were modified at their outer silica surface with isocyanate, trimethylammonium and β-cyclodextrin moieties to immobilize laccase from Trametes versicolor through covalent, electrostatic and supramolecular interactions, respectively, with protein immobilization yields ranging from 21.7 % to 53.5 %. The effect of the immobilization approach on the activity, optimal working conditions, stability and reusability of the resulting biocatalysts were studied. Best results were achieved for native and adamantane-modified laccase supramolecularly immobilized on β-cyclodextrin bearing supports in terms of their catalytic properties, showing 18.0 U and 14.0 U of immobilized laccase activity per gram of support. However, high thermal stability was observed for the enzyme covalently immobilized on isocyanate-modified nanoparticles, with 14.8-fold increase in the half-life time at 65ºC in comparison with native laccase. Best reusability properties were also achieved by covalent immobilization, retaining over 88 % of the initial catalytic activity after 13 cycles of magnetic reuses. All enzyme derivatives were evaluated for the catalytic degradation of methylene blue as pollutant model, showing significant reduction of the dye. In special, a 68-fold increase in the removal efficacy was observed for covalently immobilized enzyme compared to the free laccase. These results suggest high potential application of these biocatalysts in wastewater treatment.
{"title":"Immobilization of laccase on Fe3O4@SiO2 core@shell magnetic nanoparticles for methylene blue biodegradation","authors":"Kholoud R.M. Oraby , Anabel Villalonga , Fatma S.M. Hassan , Mohamed A. Zayed , Mahmoud F. Mubarak , Irene Ojeda , Alfredo Sánchez , Reynaldo Villalonga","doi":"10.1016/j.procbio.2024.11.012","DOIUrl":"10.1016/j.procbio.2024.11.012","url":null,"abstract":"<div><div>Here we report the preparation and characterization of novel enzyme supports based on silica-coated Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles. These nanomaterials were modified at their outer silica surface with isocyanate, trimethylammonium and β-cyclodextrin moieties to immobilize laccase from <em>Trametes versicolor</em> through covalent, electrostatic and supramolecular interactions, respectively, with protein immobilization yields ranging from 21.7 % to 53.5 %. The effect of the immobilization approach on the activity, optimal working conditions, stability and reusability of the resulting biocatalysts were studied. Best results were achieved for native and adamantane-modified laccase supramolecularly immobilized on β-cyclodextrin bearing supports in terms of their catalytic properties, showing 18.0 U and 14.0 U of immobilized laccase activity per gram of support. However, high thermal stability was observed for the enzyme covalently immobilized on isocyanate-modified nanoparticles, with 14.8-fold increase in the half-life time at 65ºC in comparison with native laccase. Best reusability properties were also achieved by covalent immobilization, retaining over 88 % of the initial catalytic activity after 13 cycles of magnetic reuses. All enzyme derivatives were evaluated for the catalytic degradation of methylene blue as pollutant model, showing significant reduction of the dye. In special, a 68-fold increase in the removal efficacy was observed for covalently immobilized enzyme compared to the free laccase. These results suggest high potential application of these biocatalysts in wastewater treatment.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 10-16"},"PeriodicalIF":3.7,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-07DOI: 10.1016/j.procbio.2024.11.007
Jinfan Ou , Xue Ren , Bangting Yin , Xu Zhang , Jian Zhou , Guiqiang He
In this study, a combined physiological and proteomic analysis was performed to investigate the effect of phosphate addition on uranium adsorption response of Kocuria rosea. When 0.4–5 g/L KH2PO4 was added to the culture medium, there was no significant change in biomass compared with the control (without KH2PO4 addition). Subsequently, the cells were collected and interacted with uranium solution (300 mg/L, 20 mL), and the adsorption rate of uranium was significantly increased from 22.14 % to 65.32 % with 3.0 g/L KH2PO4 addition. Meanwhile, the cells could release more phosphorus-containing substances and form thin film like uranium precipitates on the cell surface by fourier transform infrared spectroscopy and scanning electron microscopy characterization analyses. Furthermore, a proteomic approach was employed to reveal the regulation mechanism of phosphate on uranium interaction in the K. rosea cells. The bioinformatics analysis revealed that the differentially abundant proteins in K. rosea were mainly involved in cell motility, ribosomes, structural molecule activity, flagellar assembly, and energy metabolism under uranium stress. Interestingly, up-regulated proteins were significantly enriched for organic acid biosynthetic process in the cells with KH2PO4 addition. In addition, KH2PO4 led to upregulation of proteins including phosphohydrolase, asparagine synthase, and the phosphotransferase system (PTS) transporter subunit EIIC, which related to phosphate metabolism for regulation of uranium mineralization.
{"title":"Improvement of uranium adsorption in Kocuria rosea by phosphate: A combined physiological and proteomic analysis","authors":"Jinfan Ou , Xue Ren , Bangting Yin , Xu Zhang , Jian Zhou , Guiqiang He","doi":"10.1016/j.procbio.2024.11.007","DOIUrl":"10.1016/j.procbio.2024.11.007","url":null,"abstract":"<div><div>In this study, a combined physiological and proteomic analysis was performed to investigate the effect of phosphate addition on uranium adsorption response of <em>Kocuria rosea</em>. When 0.4–5 g/L KH<sub>2</sub>PO<sub>4</sub> was added to the culture medium, there was no significant change in biomass compared with the control (without KH<sub>2</sub>PO<sub>4</sub> addition). Subsequently, the cells were collected and interacted with uranium solution (300 mg/L, 20 mL), and the adsorption rate of uranium was significantly increased from 22.14 % to 65.32 % with 3.0 g/L KH<sub>2</sub>PO<sub>4</sub> addition. Meanwhile, the cells could release more phosphorus-containing substances and form thin film like uranium precipitates on the cell surface by fourier transform infrared spectroscopy and scanning electron microscopy characterization analyses. Furthermore, a proteomic approach was employed to reveal the regulation mechanism of phosphate on uranium interaction in the <em>K. rosea</em> cells. The bioinformatics analysis revealed that the differentially abundant proteins in <em>K. rosea</em> were mainly involved in cell motility, ribosomes, structural molecule activity, flagellar assembly, and energy metabolism under uranium stress. Interestingly, up-regulated proteins were significantly enriched for organic acid biosynthetic process in the cells with KH<sub>2</sub>PO<sub>4</sub> addition. In addition, KH<sub>2</sub>PO<sub>4</sub> led to upregulation of proteins including phosphohydrolase, asparagine synthase, and the phosphotransferase system (PTS) transporter subunit EIIC, which related to phosphate metabolism for regulation of uranium mineralization.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 569-579"},"PeriodicalIF":3.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ophiocordyceps gracilis (O. gracilis), the same genus as Ophiocordyceps sinensis, has been used as an edible medicinal fungus for many years. However, the active ingredients of O. gracilis polysaccharides remain relatively unknown. To address this, a 310.1 kDa novel polysaccharide (PDP-1a) was isolated from O. gracilis. The structural characteristics of PDP-1a were determined by different spectroscopy analyses, revealing that PDP-1a was α-glucan homologs with →4)-α-Glcp(1→ as the main chain. Furthermore, PDP-1a inhibited tumor cell proliferation, particularly in the A549 cell line, with a cell death mechanism involving cell cycle blockage, mitochondrial membrane potential reduction, and increased reactive oxygen species, thereby leading to apoptosis. Importantly, PDP-1a promotes antitumor cytokine secretion and enhances macrophage phagocytosis concentration-dependently. These findings provide a theoretical foundation for the use of O. gracilis and emphasize the potential application of PDP-1a in antitumor immunomodulatory therapy.
{"title":"Structural characterization and potential antitumor and immunostimulatory activities of mycelial polysaccharides from Ophiocordyceps gracilis","authors":"Lingling Tong, Xinya Qi, Hui Lian, Haisheng Liu, Zilei Chen, Linhui Yang, Bo Ren, Suxing Jin, Dongsheng Guo","doi":"10.1016/j.procbio.2024.11.001","DOIUrl":"10.1016/j.procbio.2024.11.001","url":null,"abstract":"<div><div><em>Ophiocordyceps gracilis (O. gracilis)</em>, the same genus as <em>Ophiocordyceps sinensis</em>, has been used as an edible medicinal fungus for many years. However, the active ingredients of <em>O. gracilis</em> polysaccharides remain relatively unknown. To address this, a 310.1 kDa novel polysaccharide (PDP-1a) was isolated from <em>O. gracilis</em>. The structural characteristics of PDP-1a were determined by different spectroscopy analyses, revealing that PDP-1a was α-glucan homologs with →4)-α-Glc<em>p</em>(1→ as the main chain. Furthermore, PDP-1a inhibited tumor cell proliferation, particularly in the A549 cell line, with a cell death mechanism involving cell cycle blockage, mitochondrial membrane potential reduction, and increased reactive oxygen species, thereby leading to apoptosis. Importantly, PDP-1a promotes antitumor cytokine secretion and enhances macrophage phagocytosis concentration-dependently. These findings provide a theoretical foundation for the use of <em>O. gracilis</em> and emphasize the potential application of PDP-1a in antitumor immunomodulatory therapy.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"148 ","pages":"Pages 1-9"},"PeriodicalIF":3.7,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142660703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-06DOI: 10.1016/j.procbio.2024.11.006
Long Zhang , Jinna Cui , Wei Liu , Zhanying Liu
Various protein secretion systems of gram-negative Escherichia coli (E. coli) are well known, but the secretion pathway of endoglucanase (BcsZ) is unknown. To further elucidate the secretion mechanism, we analyzed the transcriptome data of five ruminal cellulolytic E. coli strains. Gene Ontology (GO) enrichment analysis revealed that the differentially expressed genes (DEGs) upregulated in all five strains were significantly enriched in the membrane. Nine DEGs related to membrane transporters were overexpressed in E. coli JBZ-DH5α (overexpressing BcsZ in the genome of E. coli DH5α). The results revealed that the overexpression of the b4332 (yjiJ), b2142 (yohK), and b2377 (yfdY) genes significantly reduced the growth and BcsZ concentration of the strain. The overexpression of the genes b3533 (bcsA), b3009 (yghB), and b3522 (yhjD) did not significantly affect strain growth or the BcsZ concentration. The overexpression of the b1859 (znuB), b0458 (ylaC), and b1195 (ymgE) genes increased the secretion of BcsZ but had no significant effect on strain growth. In conclusion, the yjiJ, yohK, yfdY, znuB, ylaC, and ymgE genes are involved in the secretion of E. coli BcsZ. These findings provide insight into the secretion mechanism of E. coli BcsZ.
{"title":"Impact of membrane gene overexpression on endoglucanase secretion in ruminal cellulolytic Escherichia coli","authors":"Long Zhang , Jinna Cui , Wei Liu , Zhanying Liu","doi":"10.1016/j.procbio.2024.11.006","DOIUrl":"10.1016/j.procbio.2024.11.006","url":null,"abstract":"<div><div>Various protein secretion systems of gram-negative <em>Escherichia coli</em> (<em>E. coli</em>) are well known, but the secretion pathway of endoglucanase (BcsZ) is unknown. To further elucidate the secretion mechanism, we analyzed the transcriptome data of five ruminal cellulolytic <em>E. coli</em> strains. Gene Ontology (GO) enrichment analysis revealed that the differentially expressed genes (DEGs) upregulated in all five strains were significantly enriched in the membrane. Nine DEGs related to membrane transporters were overexpressed in <em>E. coli</em> JBZ-DH5α (overexpressing BcsZ in the genome of <em>E. coli</em> DH5α). The results revealed that the overexpression of the b4332 (<em>yjiJ</em>), b2142 (<em>yohK</em>), and b2377 (<em>yfdY</em>) genes significantly reduced the growth and BcsZ concentration of the strain. The overexpression of the genes b3533 (<em>bcsA</em>), b3009 (<em>yghB</em>), and b3522 (<em>yhjD</em>) did not significantly affect strain growth or the BcsZ concentration. The overexpression of the b1859 (<em>znuB</em>), b0458 (<em>ylaC</em>), and b1195 (<em>ymgE</em>) genes increased the secretion of BcsZ but had no significant effect on strain growth. In conclusion, the <em>yjiJ</em>, <em>yohK</em>, <em>yfdY</em>, <em>znuB</em>, <em>ylaC</em>, and <em>ymgE</em> genes are involved in the secretion of <em>E. coli</em> BcsZ. These findings provide insight into the secretion mechanism of <em>E. coli</em> BcsZ.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 580-586"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-06DOI: 10.1016/j.procbio.2024.11.005
Luis Felipe Cuesta-Zedeño , Ramón Alberto Batista-García , Nina Gunde-Cimerman , Leonel Ernesto Amábilis-Sosa , Blenda Ramirez-Pereda
A single-chamber microbial fuel cell (MFC) was constructed to assess the capacity of Exophiala dermatitidis EXF-8193 as an electron-donating microorganism. Unidirectional carbon fiber electrodes were used, and voltage generation was monitored over 120 hours. The system achieved a maximum voltage of 176 mV after 93 hours of operation. Simultaneously, the decolorization of Basic Blue 9 (BB9) dye was evaluated, achieving 70% degradation within 120 hours. Initial optimization studies focused on individual variables, including carbon source, anode shape, and anodic surface area. Results indicated that glucose, a T-shaped anode, and an anodic area of 12 cm² were optimal, yielding voltage values of 175.8 ± 0.57 mV, 175.8 ± 0.57 mV, and 310.53 ± 1.22 mV, respectively. In the second stage, a multiparametric optimization was conducted using Response Surface Methodology (RSM) with a Box-Behnken design, resulting in a second-order model with an R2 of 91.7. Under optimized conditions, the MFC reached a favorable maximum voltage of 284 mV, demonstrating enhanced performance with fine-tuned operational parameters. These findings represent a pioneering step in exploring black yeast, particularly E. dermatitidis, as a sustainable bio-catalyst in MFC technology. This study opens new avenues for further research on extremophilic fungi in bioenergy production and wastewater treatment, highlighting the need for continued exploration of black yeast's unique properties in biotechnological applications.
{"title":"Utilizing black yeast for sustainable solutions: Pioneering clean energy production and wastewater treatment with Exophiala dermatitidis","authors":"Luis Felipe Cuesta-Zedeño , Ramón Alberto Batista-García , Nina Gunde-Cimerman , Leonel Ernesto Amábilis-Sosa , Blenda Ramirez-Pereda","doi":"10.1016/j.procbio.2024.11.005","DOIUrl":"10.1016/j.procbio.2024.11.005","url":null,"abstract":"<div><div>A single-chamber microbial fuel cell (MFC) was constructed to assess the capacity of <em>Exophiala dermatitidis</em> EXF-8193 as an electron-donating microorganism. Unidirectional carbon fiber electrodes were used, and voltage generation was monitored over 120 hours. The system achieved a maximum voltage of 176 mV after 93 hours of operation. Simultaneously, the decolorization of Basic Blue 9 (BB9) dye was evaluated, achieving 70% degradation within 120 hours. Initial optimization studies focused on individual variables, including carbon source, anode shape, and anodic surface area. Results indicated that glucose, a T-shaped anode, and an anodic area of 12 cm² were optimal, yielding voltage values of 175.8 ± 0.57 mV, 175.8 ± 0.57 mV, and 310.53 ± 1.22 mV, respectively. In the second stage, a multiparametric optimization was conducted using Response Surface Methodology (RSM) with a Box-Behnken design, resulting in a second-order model with an R<sup>2</sup> of 91.7. Under optimized conditions, the MFC reached a favorable maximum voltage of 284 mV, demonstrating enhanced performance with fine-tuned operational parameters. These findings represent a pioneering step in exploring black yeast, particularly <em>E. dermatitidis</em>, as a sustainable bio-catalyst in MFC technology. This study opens new avenues for further research on extremophilic fungi in bioenergy production and wastewater treatment, highlighting the need for continued exploration of black yeast's unique properties in biotechnological applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 630-643"},"PeriodicalIF":3.7,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nanoparticles (NPs) are being used in the rapidly developing field of nanotechnology. Nanotechnology research involves manipulating materials at the nano-scale to create new structures and devices that are beneficial to humans. Owing to their unique characteristics, nanomaterials have revolutionized numerous sectors and sped up numerous scientific breakthroughs while remaining at the nanometer scale. Research in this area is flourishing and of great interest to scientists. The present review offers researchers a comprehensive introduction to nanotechnology, focusing on nanoparticle biosynthesis routes, factors influencing the synthesis process, characterization methods, and current applications. Microbial nanobiotechnology, is a fast-growing area of research that holds the potential to revolutionize diverse fields such as bioremediation, energy generation, healthcare, and agriculture. Biological manufacturing of nanoparticles is more feasible than chemical synthesis, which can be costly and result in harmful repercussions. Microbes, particularly bacteria, are among the best options for the efficient production of nanoparticles because of their rapid growth rate, ability to be manipulated through genetics to achieve optimum efficiency, and relative lack of both expense and toxicity. It specifically explores the microbe-mediated biological synthesis of nanoparticles. This article presents an updated understanding of how biological synthesis can support innovative nanotechnology applications.
{"title":"Current state and future prospects of microbiologically produced nanoparticles: A narrative review","authors":"B. Kiran Sharma , Balakumaran Manickam Dakshinamoorthi , Manjunathan Jagadeesan , Saravanan Sekaran , Ambiga Somasundaram , S. Jagadeeswari , Pasiyappazham Ramasamy","doi":"10.1016/j.procbio.2024.11.003","DOIUrl":"10.1016/j.procbio.2024.11.003","url":null,"abstract":"<div><div>Nanoparticles (NPs) are being used in the rapidly developing field of nanotechnology. Nanotechnology research involves manipulating materials at the nano-scale to create new structures and devices that are beneficial to humans. Owing to their unique characteristics, nanomaterials have revolutionized numerous sectors and sped up numerous scientific breakthroughs while remaining at the nanometer scale. Research in this area is flourishing and of great interest to scientists. The present review offers researchers a comprehensive introduction to nanotechnology, focusing on nanoparticle biosynthesis routes, factors influencing the synthesis process, characterization methods, and current applications. Microbial nanobiotechnology, is a fast-growing area of research that holds the potential to revolutionize diverse fields such as bioremediation, energy generation, healthcare, and agriculture. Biological manufacturing of nanoparticles is more feasible than chemical synthesis, which can be costly and result in harmful repercussions. Microbes, particularly bacteria, are among the best options for the efficient production of nanoparticles because of their rapid growth rate, ability to be manipulated through genetics to achieve optimum efficiency, and relative lack of both expense and toxicity. It specifically explores the microbe-mediated biological synthesis of nanoparticles. This article presents an updated understanding of how biological synthesis can support innovative nanotechnology applications.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 554-568"},"PeriodicalIF":3.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142663437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-05DOI: 10.1016/j.procbio.2024.11.004
Rundong Yang , Siyan Liu , Feifei Wang , Shuyi Li , Na Zhang , Zhenzhou Zhu
Laminaria polysaccharides (LP) have been shown to effectively stabilize selenium nanoparticles (SeNPs), forming LP–SeNP complexes with enhanced bioactivity. However, the correlation between their bioactivity and physicochemical properties remains inadequately explored. This study used chemical reduction with LP as stabilizer to investigate how LP-to-selenium mass ratio (LPSMR), reaction time, and temperature influence particle size, selenium content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of LP–SeNPs. The results showed that enhanced DPPH scavenging correlated with higher selenium content and smaller particle size, primarily modulated by LPSMR and temperature. Under optimal conditions (LPSMR of 1; temperature of 25°C), the resulting LP–SeNPs exhibited uniform morphology with a particle size of 81.41 nm and selenium content of 653.91 mg/g. This stability was achieved through non-covalent interactions between LP and SeNPs, providing superior light and acid resistance compared to unmodified SeNPs. Notably, LP–SeNPs showed synergistic antioxidant effects, with lower half-maximal inhibitory concentration (IC50) values for scavenging DPPH, hydroxyl, and superoxide anion radicals than LP or SeNPs alone, and enhanced hypoglycemic activity. Cytotoxicity assays confirmed LP–SeNPs had reduced toxicity compared to Na2SeO3 and selenopeptide. These findings provide insights into the structure-activity relationships of LP–SeNPs and support their potential application as antioxidant and hypoglycemic agents.
{"title":"Construction and characterization of Laminaria polysaccharide functionalized selenium nanoparticles based on an activity-oriented approach","authors":"Rundong Yang , Siyan Liu , Feifei Wang , Shuyi Li , Na Zhang , Zhenzhou Zhu","doi":"10.1016/j.procbio.2024.11.004","DOIUrl":"10.1016/j.procbio.2024.11.004","url":null,"abstract":"<div><div><em>Laminaria</em> polysaccharides (LP) have been shown to effectively stabilize selenium nanoparticles (SeNPs), forming LP–SeNP complexes with enhanced bioactivity. However, the correlation between their bioactivity and physicochemical properties remains inadequately explored. This study used chemical reduction with LP as stabilizer to investigate how LP-to-selenium mass ratio (LPSMR), reaction time, and temperature influence particle size, selenium content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of LP–SeNPs. The results showed that enhanced DPPH scavenging correlated with higher selenium content and smaller particle size, primarily modulated by LPSMR and temperature. Under optimal conditions (LPSMR of 1; temperature of 25°C), the resulting LP–SeNPs exhibited uniform morphology with a particle size of 81.41 nm and selenium content of 653.91 mg/g. This stability was achieved through non-covalent interactions between LP and SeNPs, providing superior light and acid resistance compared to unmodified SeNPs. Notably, LP–SeNPs showed synergistic antioxidant effects, with lower half-maximal inhibitory concentration (IC<sub>50</sub>) values for scavenging DPPH, hydroxyl, and superoxide anion radicals than LP or SeNPs alone, and enhanced hypoglycemic activity. Cytotoxicity assays confirmed LP–SeNPs had reduced toxicity compared to Na<sub>2</sub>SeO<sub>3</sub> and selenopeptide. These findings provide insights into the structure-activity relationships of LP–SeNPs and support their potential application as antioxidant and hypoglycemic agents.</div></div>","PeriodicalId":20811,"journal":{"name":"Process Biochemistry","volume":"147 ","pages":"Pages 544-553"},"PeriodicalIF":3.7,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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