Genome-wide analysis of SUMO conjugation pathway members in broccoli and the involvement of BoSIZ1 in response to ABA

IF 4 3区 生物学 Q1 PLANT SCIENCES Journal of plant physiology Pub Date : 2025-03-04 DOI:10.1016/j.jplph.2025.154472
Sibo Wang, Yukai Ji, Jing Han, Jingsong Guo, Xiaoxue Hu, Wei Ji
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Abstract

The small ubiquitin-like protein modifier (SUMO) is a conserved protein that modifies target proteins by attaching to them, changing their functions, localizations, and interactions. However, there is limited knowledge regarding the process of SUMOylation in broccoli (Brassica oleracea var. italica), a highly nutritious vegetable that is widely consumed. In this study, a total of 40 genes including 6 families associated with the SUMOylation pathway were identified in the broccoli genome. Western blot analysis using AtSUMO1 antibody showed that SUMOylation levels increased as broccoli sprouts grew, peaking at 11 days when true leaves were fully developed. RT-qPCR analysis of 10 SUMO pathway genes showed that most of them were upregulated in response to high temperature, NaCl, and abscisic acid (ABA) stimuli within 24 h. Western blot analysis showed changes in SUMOylation dynamics in broccoli sprouts under abiotic stress conditions, regulating SUMOylated proteins. The nuclear localization of the SUMO E3 ligase BoSIZ1a was determined, along with its SUMOylation activity in vivo. Overexpression of BoSIZ1a in Arabidopsis resulted in reduced sensitivity to ABA and decreased expression of ABA-responsive genes (AtABF3, AtADH, AtEm6, AtABI5, AtRAB18, and AtRD29A). Collectively, this study reveals the organization of the broccoli SUMOylation system and highlights the crucial function of SUMOylation in broccoli's response to abiotic stress, as well as the significant contribution of BoSIZ1a in the plant's ABA response.
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小泛素样蛋白修饰子(SUMO)是一种保守的蛋白质,它通过附着在目标蛋白质上对其进行修饰,从而改变其功能、定位和相互作用。然而,人们对西兰花(Brassica oleracea var. italica)这种广泛食用的高营养蔬菜的 SUMO 化过程了解有限。本研究在西兰花基因组中发现了包括 6 个家族在内的 40 个与 SUMOylation 通路相关的基因。使用 AtSUMO1 抗体进行的 Western 印迹分析表明,SUMOylation 水平随着西兰花芽的生长而增加,在真叶完全发育 11 天时达到顶峰。对 10 个 SUMO 通路基因的 RT-qPCR 分析表明,在高温、NaCl 和脱落酸(ABA)刺激下,大部分基因在 24 小时内上调。测定了 SUMO E3 连接酶 BoSIZ1a 的核定位及其在体内的 SUMO 化活性。在拟南芥中过表达 BoSIZ1a 会降低对 ABA 的敏感性,并减少 ABA 响应基因(AtABF3、AtADH、AtEm6、AtABI5、AtRAB18 和 AtRD29A)的表达。总之,本研究揭示了西兰花 SUMOylation 系统的组织结构,并强调了 SUMOylation 在西兰花对非生物胁迫的响应中的关键功能,以及 BoSIZ1a 在植物 ABA 响应中的重要贡献。
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来源期刊
Journal of plant physiology
Journal of plant physiology 生物-植物科学
CiteScore
7.20
自引率
4.70%
发文量
196
审稿时长
32 days
期刊介绍: The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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