Synthesis, optical properties, theoretical calculations, and related applications of small molecule fluorescent probes

IF 3.6 3区 物理与天体物理 Q2 OPTICS Journal of Luminescence Pub Date : 2025-06-01 Epub Date: 2025-03-06 DOI:10.1016/j.jlumin.2025.121177
Jinghua Zhang , Yue Tang , Xiaoqiang Tong , Qichuan Duan , Shi Jin , Jun Zheng , Guoliang Xie , Dingmei Qin , Shangrong Zhang , Deqian Huang , Feng Jin
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Abstract

Small molecule fluorescent probes are important chemical tools for studying the localization of fluorescent probes and their surrounding environmental characteristics in fields such as biochemistry, cell imaging, and materials science. Two novel fluorescent probes based on carbazole fluorophores were designed and synthesized in this study. The optical properties of P1 and P2 were studied by UV visible absorption spectroscopy, one-photon excited fluorescence spectroscopy, and two-photon excited fluorescence spectroscopy. Meanwhile, the results of cell imaging experiments demonstrate that P1 exhibits good cell permeability and low cell toxicity, and has excellent imaging and mitochondrial localization abilities in cells. In addition, detailed spectral analysis of P2 under acidic and alkaline conditions was conducted, revealing its acid-induced color-change property in solutions. Based on its acid-base sensing characteristics, P2 demonstrates potential applications in the field of anti-counterfeiting. The design and application of P1 and P2 provide valuable information for the development of more carbazole-based fluorescent probes.
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小分子荧光探针的合成、光学性质、理论计算及相关应用
小分子荧光探针是生物化学、细胞成像、材料科学等领域研究荧光探针定位及其周围环境特性的重要化学工具。设计并合成了两种基于咔唑荧光团的新型荧光探针。利用紫外可见吸收光谱、单光子激发荧光光谱和双光子激发荧光光谱研究了P1和P2的光学性质。同时,细胞成像实验结果表明,P1具有良好的细胞通透性和低细胞毒性,在细胞内具有优异的成像和线粒体定位能力。此外,对P2在酸性和碱性条件下进行了详细的光谱分析,揭示了其在溶液中的酸致变色特性。基于其酸碱传感特性,P2在防伪领域具有潜在的应用前景。P1和P2的设计和应用为开发更多基于咔唑的荧光探针提供了有价值的信息。
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来源期刊
Journal of Luminescence
Journal of Luminescence 物理-光学
CiteScore
6.70
自引率
13.90%
发文量
850
审稿时长
3.8 months
期刊介绍: The purpose of the Journal of Luminescence is to provide a means of communication between scientists in different disciplines who share a common interest in the electronic excited states of molecular, ionic and covalent systems, whether crystalline, amorphous, or liquid. We invite original papers and reviews on such subjects as: exciton and polariton dynamics, dynamics of localized excited states, energy and charge transport in ordered and disordered systems, radiative and non-radiative recombination, relaxation processes, vibronic interactions in electronic excited states, photochemistry in condensed systems, excited state resonance, double resonance, spin dynamics, selective excitation spectroscopy, hole burning, coherent processes in excited states, (e.g. coherent optical transients, photon echoes, transient gratings), multiphoton processes, optical bistability, photochromism, and new techniques for the study of excited states. This list is not intended to be exhaustive. Papers in the traditional areas of optical spectroscopy (absorption, MCD, luminescence, Raman scattering) are welcome. Papers on applications (phosphors, scintillators, electro- and cathodo-luminescence, radiography, bioimaging, solar energy, energy conversion, etc.) are also welcome if they present results of scientific, rather than only technological interest. However, papers containing purely theoretical results, not related to phenomena in the excited states, as well as papers using luminescence spectroscopy to perform routine analytical chemistry or biochemistry procedures, are outside the scope of the journal. Some exceptions will be possible at the discretion of the editors.
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