Synthesis of trypsin-protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-03-10 DOI:10.1007/s00604-025-07070-8

Suresh Kumar Kailasa, Kartik Pankajbhai Makwana, Madhura Pradeep Deshpande, Yoojin Choi, Ruth Stephanie, Chan Yeong Park, Tae Jung Park

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Abstract

Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for molecular species sensing in aqueous media. Here, we introduce trypsin (Try) as a bioligand for the synthesis of cesium lead chloride (CsPbCl₃) PNCs with high water stability. The as-fabricated Try-CsPbCl₃ PNCs show λ_Em/Ex at 433/370 nm with a quantum yield of 17.26%. The fluorescence emission spectral characteristics of Try-CsPbCl₃ PNCs demonstrated that water-stable Try-CsPbCl₃ PNCs acted as a promising fluorescent probe for the detection of hydroxyl radical (^•OH) via turn-off mechanism. The Try-CsPbCl₃ PNCs-based turn-off fluorescence approach displayed good selectivity for hydroxyl radical in water, showing a wider linear range (0.01–5 µM) with a remarkable detection limit of 3.10 nM for hydroxyl radical. The Try-CsPbCl₃ PNCs were demonstrated to be a facile probe for sensing ^•OH in water samples, which signifies that Try-CsPbCl₃ PNCs exhibited broad applications for hydroxyl radical sensing in real samples.

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.