Genome Mining of Epicoccum dendrobii Reveals Diverse Antimicrobial Natural Products

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agricultural and Food Chemistry Pub Date : 2025-03-10 DOI:10.1021/acs.jafc.4c11808
Anxin Zhang, Xinran Xu, Wen-Bing Yin
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Abstract

Epicoccum dendrobii, a biocontrol fungus, produces bioactive metabolites with potential agricultural applications. Targeted genome mining revealed 13 compounds, including three novel polyketides (epiketides A–C, 13), a new natural product (epiketide D, 4), a diketopiperazine (boydine E, 5), and eight known analogues 613. Structures were elucidated via NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and computational methods. Compounds 1, 2 and 4 exhibited moderate antibacterial activity against Bacillus licheniformis (minimum inhibitory concentrations (MICs): 5.5–37.6 μM) and Pseudomonas aeruginosa (MICs: 11.1–20.0 μM). A protoplast transformation system was developed to identify the biosynthetic gene cluster eda responsible for diketopiperazines 510. Antifungal assays revealed that wild-type E. dendrobii inhibited plant pathogen Botrytis cinerea by 56.03%, while the ΔedaP mutant (lacking diketopiperazines) showed reduced efficacy, implying the role of diketopiperazines in antifungal activity. This study underscores E. dendrobii’s potential as a biocontrol agent and provides insights into its bioactive metabolites for agricultural applications.

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Epicoccum dendrobii 是一种生物防治真菌,可产生具有潜在农业应用价值的生物活性代谢物。靶向基因组挖掘发现了 13 种化合物,包括三种新型多酮(表酮酰胺 A-C,1-3)、一种新的天然产物(表酮酰胺 D,4)、一种二酮哌嗪(波依定 E,5)和 8 种已知类似物 6-13。通过核磁共振、高分辨率电喷雾质谱(HR-ESI-MS)和计算方法阐明了这些化合物的结构。化合物 1、2 和 4 对地衣芽孢杆菌(最低抑菌浓度:5.5-37.6 μM)和铜绿假单胞菌(最低抑菌浓度:11.1-20.0 μM)具有中等程度的抗菌活性。开发了一种原生质体转化系统,以确定 5-10 号二酮哌嗪的生物合成基因簇 eda。抗真菌实验表明,野生型 E. dendrobii 对植物病原菌 Botrytis cinerea 的抑制率为 56.03%,而 ΔedaP 突变体(缺乏二酮哌嗪)的抑制率降低,这意味着二酮哌嗪在抗真菌活性中的作用。这项研究强调了 E. dendrobii 作为生物控制剂的潜力,并为其生物活性代谢物在农业上的应用提供了深入的见解。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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