Construction and application of magnetic surface molecularly imprinted solid-phase extraction for the detection of 5-hydroxytryptamine in peripheral blood

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-03-11 DOI:10.1007/s00604-025-07058-4

Gege Zhu, Xinyu Li, Qianjie Ma, Zhanying Hong, Dan Zhong, Xiaodong Sun

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Abstract

The 5-Hydroxytryptamine (5-HT) level determination is crucial for predicting, pathogenesis, diagnosis, and pharmacological treatment of schizophrenia. To realize the extraction of trace 5-HT in complex matrix samples, a magnetic molecularly imprinted solid-phase extraction (MMISPE) pretreatment method was developed. In combination with UPLC-MS/MS, the method was possible to achieve the quantification of 1 ng/mL 5-HT in tissue samples. The type of adsorbent, magnetic surface molecularly imprinted polymers (MMIPs) adsorbent dosage, adsorption temperature, and adsorption method were screened to obtain the better extraction of 5-HT. After optimizing the extraction and separation method, we applied the MMISPE method to the detection of 5-HT in peripheral blood of clinical schizophrenia patients. The results showed that the average concentration of 5-HT in the peripheral blood of healthy controls was 74.30 ng/mL, whereas the 5-HT content in the samples from schizophrenic patients was 1.17 ng/mL.

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磁性表面分子印迹固相萃取法检测外周血5-羟色胺的构建及应用

5-羟色胺（5-HT）水平的测定对精神分裂症的预测、发病机制、诊断和药物治疗至关重要。为了实现复杂基质样品中痕量5-羟色胺的提取，建立了磁性分子印迹固相萃取（MMISPE）预处理方法。结合UPLC-MS/MS，该方法可实现组织样品中1 ng/mL 5-HT的定量。通过对吸附剂类型、磁性表面分子印迹聚合物（MMIPs）吸附剂用量、吸附温度和吸附方法的筛选，获得了较好的5-HT萃取效果。在优化提取分离方法后，我们将MMISPE法应用于临床精神分裂症患者外周血5-HT的检测。结果表明，正常人外周血5-HT平均浓度为74.30 ng/mL，精神分裂症患者外周血5-HT平均浓度为1.17 ng/mL。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.