Microfluidic development of liposome nanoparticles encapsulated with yam polysaccharide.

IF 3.7 3区 医学 Q2 CHEMISTRY, MEDICINAL Journal of pharmaceutical sciences Pub Date : 2025-03-05 DOI:10.1016/j.xphs.2025.103718
Yuehan Cui, Meng Song, Ruilai Liu, Zhenhao Xi, Ling Zhao, Lian Cen
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Abstract

A novel liposome nanoparticle loaded with yam polysaccharide was developed in this study via a tailor-made microfluidic device and further optimized through the Box-Behnken Design method of surface analysis to exploit combinational immunomodulatory effects of liposomes and yam polysaccharide. A chip of 100 μm in height and 200 μm in width with a mixing channel with embedded baffles to enhance fluid mixing was first designed and fabricated. Liposome nanoparticles were obtained by manipulating the operating parameters of the microfluidic chip. The formulation of yam polysaccharide loaded liposomes (YPL1) was optimized using response-surface analysis, and their physicochemical properties were characterized. In vitro cellular assays were performed to assess the effect of YPL on the activity of mouse dendritic cells (DC) and the secretion of immune-related cytokines to investigate the immune-enhancing effect of YPL in vitro. The resulting YPL had a mean size of 154.2±3.8 nm with a narrow size distribution (PDI=0.083) and had a high entrapment efficiency (EE) of 79.02 %. The YPL exhibited good stability at 4 °C over 14 days of storage, and an in vitro sustained release duration of approximately 30 h. The YPL demonstrated excellent biocompatibility and stimulated the expression of immune-related cytokines, especially TNF-α. Therefore, the YPL with well controlled particle size and high loading capacity, capable of exhibiting potent immunostimulatory activity was successfully developed in this study via a microfluidic device and optimized using response-surface analysis. The current YPL engineering methodology could further serve as an experimental platform for liposome nanoparticle development.

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本研究通过量身定制的微流控装置开发了一种负载山药多糖的新型脂质体纳米粒子,并通过盒-贝肯设计表面分析方法对其进行了进一步优化,以利用脂质体和山药多糖的组合免疫调节效应。首先设计并制作了一个高 100 微米、宽 200 微米的芯片,芯片上有一个混合通道,通道内嵌挡板以加强流体混合。通过调节微流控芯片的工作参数,获得了脂质体纳米颗粒。利用响应面分析法优化了山药多糖负载脂质体(YPL1)的配方,并对其理化性质进行了表征。通过体外细胞试验评估了山药多糖对小鼠树突状细胞(DC)活性和免疫相关细胞因子分泌的影响,以研究山药多糖的体外免疫增强作用。结果表明,YPL 的平均粒径为 154.2±3.8 nm,粒径分布较窄(PDI=0.083),夹带效率(EE)高达 79.02%。YPL 在 4°C 下储存 14 天后表现出良好的稳定性,体外持续释放时间约为 30 小时。YPL 具有良好的生物相容性,并能刺激免疫相关细胞因子的表达,尤其是 TNF-α。因此,本研究通过微流控装置成功开发出了粒径可控、负载能力强、具有强效免疫刺激活性的 YPL,并利用响应面分析法对其进行了优化。目前的 YPL 工程方法可进一步作为脂质体纳米粒子开发的实验平台。
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来源期刊
CiteScore
7.30
自引率
13.20%
发文量
367
审稿时长
33 days
期刊介绍: The Journal of Pharmaceutical Sciences will publish original research papers, original research notes, invited topical reviews (including Minireviews), and editorial commentary and news. The area of focus shall be concepts in basic pharmaceutical science and such topics as chemical processing of pharmaceuticals, including crystallization, lyophilization, chemical stability of drugs, pharmacokinetics, biopharmaceutics, pharmacodynamics, pro-drug developments, metabolic disposition of bioactive agents, dosage form design, protein-peptide chemistry and biotechnology specifically as these relate to pharmaceutical technology, and targeted drug delivery.
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