Hemocyanin-derived antimicrobial peptide PvL1 defense against AHPND infection by regulating the hepatopancreatic microbiota of Penaeus vannamei

IF 4.1 2区农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2025-03-08 DOI:10.1016/j.fsi.2025.110267

Maoshuai Fu , Minrui Liao , Yingmei Qin , Lixuan He , Zhihong Zheng , Yongzhen Zhao , Qingyun Liu , Yueling Zhang , Xianliang Zhao

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Abstract

Antimicrobial peptides (AMPs) play a crucial role in maintaining the balance of host microbiota, serving as the first line of defense against pathogenic microorganisms. However, the potential mechanisms by which AMPs modulate microbial homeostasis are not fully understood. In this study, we showed that Vibrio parahaemolyticus (VP_AHPND) infection could suppress the expression of hemocyanin and promote its degradation. PvL1, a hemocyanin-derived AMP, exhibited high antibacterial activity against AHPND infection. Then, it was observed that PvL1 could restore the dysbiosis in the hepatopancreas microbiota of AHPND shrimps. In addition, PvL1 could increase the survival of shrimp against VP_AHPND infection by modulating the predominance of bacteria in the hepatopancreas microbiota. Taken together, these findings highlight the crucial role of hemocyanin-derived peptides in maintaining homeostasis in the hepatopancreas microbiota in response to AHPND disease.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.