Hemocyanin-derived antimicrobial peptide PvL1 defense against AHPND infection by regulating the hepatopancreatic microbiota of Penaeus vannamei

IF 3.9 2区农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2025-06-01 Epub Date: 2025-03-08 DOI:10.1016/j.fsi.2025.110267

Maoshuai Fu , Minrui Liao , Yingmei Qin , Lixuan He , Zhihong Zheng , Yongzhen Zhao , Qingyun Liu , Yueling Zhang , Xianliang Zhao

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Abstract

Antimicrobial peptides (AMPs) play a crucial role in maintaining the balance of host microbiota, serving as the first line of defense against pathogenic microorganisms. However, the potential mechanisms by which AMPs modulate microbial homeostasis are not fully understood. In this study, we showed that Vibrio parahaemolyticus (VP_AHPND) infection could suppress the expression of hemocyanin and promote its degradation. PvL1, a hemocyanin-derived AMP, exhibited high antibacterial activity against AHPND infection. Then, it was observed that PvL1 could restore the dysbiosis in the hepatopancreas microbiota of AHPND shrimps. In addition, PvL1 could increase the survival of shrimp against VP_AHPND infection by modulating the predominance of bacteria in the hepatopancreas microbiota. Taken together, these findings highlight the crucial role of hemocyanin-derived peptides in maintaining homeostasis in the hepatopancreas microbiota in response to AHPND disease.

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血青素来源的抗菌肽PvL1通过调节凡纳滨对虾肝胰腺微生物群防御AHPND感染

抗菌肽在维持宿主菌群平衡中起着至关重要的作用，是抵御病原微生物的第一道防线。然而，amp调节微生物稳态的潜在机制尚不完全清楚。在本研究中，我们发现副溶血性弧菌（VPAHPND）感染可抑制血青素的表达并促进其降解。PvL1是一种血青素来源的AMP，对AHPND感染具有较高的抗菌活性。然后，观察PvL1对AHPND对虾肝胰腺微生物群失调的恢复作用。此外，PvL1可以通过调节肝胰腺微生物群中细菌的优势来提高对虾抗VPAHPND感染的存活率。综上所述，这些发现强调了血青素衍生肽在AHPND疾病反应中维持肝胰腺微生物群稳态中的关键作用。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.