MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: application in viral infection diagnostics

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL Microchimica Acta Pub Date : 2025-03-11 DOI:10.1007/s00604-025-07084-2

Estefanía Enebral-Romero, Emiliano Martínez-Periñán, David López-Diego, Mónica Luna, Marina Garrido, Cristina Navío, Emilio M. Pérez, Encarnación Lorenzo, Tania García-Mendiola

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Abstract

An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor’s functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identification via genetic code recognition. CNDsTy allow the electrochemical detection based on their different affinity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identification. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples.

Graphical Abstract

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用于高性能DNA生物传感器的MoS 2 -DNA四面体生物偶联物：在病毒感染诊断中的应用

提出了一种基于二硫化钼（MoS 2）、四面体DNA纳米结构（TDNs）和硫氨酸修饰碳纳米点（CNDsTy）的电化学DNA生物传感器，用于病毒感染的早期检测。这项工作的创新之处在于首次整合这些纳米材料制备生物偶联物，其协同作用使生物传感器的功能得以实现。MoS₂锚定tdn， tdn携带捕获探针，通过遗传密码识别进行病毒鉴定。CNDsTy基于其对单链DNA （ssDNA）和双链DNA （dsDNA）的不同亲和力进行电化学检测，从而实现杂交事件识别。该生物传感器具有较高的灵敏度（检测限为5.00 fM），可区分病毒载量，并通过人鼻咽样本中SARS-CoV-2 ORF1ab序列进行验证。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.