Polyvalent immunoprotection of protein, DNA and IgY antibody vaccines of Vibrio fluvialis outer membrane protein VF08100 in fish

IF 3.9 2区农林科学 Q1 FISHERIES Fish & shellfish immunology Pub Date : 2025-03-08 DOI:10.1016/j.fsi.2025.110260

Huihui Xiao , Saixing Duan , Pan Cui , Jing Chen , Xixian Che , Juan Lu , Juan Wang , Guoping Zhu , Yong Liu , Xiang Liu

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Abstract

Vaccines are of great importance to green aquaculture. In this study, the polyvalent immunoprotective activities of protein, DNA and egg yolk antibody (IgY) vaccines of Vibrio fluvialis outer membrane protein VF08100 were assessed. Carassius auratus was immunized with these three vaccines and challenged with V. fluvialis and Aeromonas hydrophila. The results showed that the three vaccines had significant immune protection rates (p < 0.01) against V. fluvialis and A. hydrophila infections, could activate the congenital immune response in C. auratus, and downregulate the expression of inflammation genes (p < 0.01) and antioxidant factors (p < 0.01) to reduce the inflammatory response and antioxidant reactions, respectively. Moreover, the three vaccines could protect the internal tissue structure integrity and reduce the apoptosis and DNA damage of kidney cells induced by bacterial infection. Therefore, the protein, DNA and IgY vaccines of VF08100 resisted multiple bacterial infections and can be used as polyvalent candidate vaccines for aquaculture.

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河弧菌外膜蛋白VF08100蛋白、DNA和IgY抗体疫苗对鱼的多价免疫保护作用。

疫苗对绿色水产养殖具有重要意义。本研究评估了流感弧菌外膜蛋白 VF08100 的蛋白、DNA 和卵黄抗体（IgY）疫苗的多价免疫保护活性。用这三种疫苗对鲫鱼进行免疫接种，并用流感弧菌和嗜水气单胞菌进行挑战。结果表明，这三种疫苗对弧菌和嗜水气单胞菌感染有显著的免疫保护率（p < 0.01），能激活鲫鱼的先天免疫反应，并分别下调炎症基因（p < 0.01）和抗氧化因子（p < 0.01）的表达，以减少炎症反应和抗氧化反应。此外，三种疫苗还能保护肾脏内部组织结构的完整性，减少细菌感染引起的肾脏细胞凋亡和DNA损伤。因此，VF08100的蛋白疫苗、DNA疫苗和IgY疫苗均能抵抗多种细菌感染，可作为多价候选疫苗用于水产养殖。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.