Analysis of Codon Usage Patterns in a Rare Palmitoleic Acid Production Oleaginous Yeast, Scheffersomyces segobiensis.

IF 2.3 3区生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2025-03-10 DOI:10.1007/s00284-025-04162-9

Lili Zhang, Wenxing Cui, Dandan Tang, Haohong Lin, Weiliang Dong, Xiujuan Qian, Min Jiang

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Abstract

Scheffersomyces segobiensis DSM27193 has been identified as a potential host for Palmitoleic acid (POA)-rich lipids production. Our previous study has found that S. segobiensis DSM27193 possesses a broad substrate spectrum, excellent xylose fermentation capability, rapid growth, high biomass, and an abundance of intracellular acetyl-CoA. These characteristics make S. segobiensis DSM27193 an ideal chassis for metabolic engineering and the synthesis of various chemicals. However, the genetic coding features of this strain remain unclear. Analysis of the internal transcribed spacer (ITS) region suggested that S. segobiensis DSM27193 is closely related to Pichia stipitis (renamed Scheffersomyces stipitis), both of which exhibit CTG coding characteristic. In this study, we utilized software tools, such as CodonW, CUPS, and others to analyze the codon preference of the S. segobiensis DSM27193 genome. Additionally, we validated the reliability of the codon table through the expression of green fluorescent protein (GFP). The analysis results serve as a theoretical foundation for enhancing the expression of exogenous genes in S. segobiensis DSM27193.

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Scheffersomyces segobiensis DSM27193已被确定为生产富棕榈油酸（POA）脂类的潜在宿主。我们之前的研究发现，S. segobiensis DSM27193 具有广泛的底物谱、出色的木糖发酵能力、快速生长、高生物量和丰富的胞内乙酰-CoA。这些特性使 S. segobiensis DSM27193 成为代谢工程和合成各种化学物质的理想底盘。然而，该菌株的基因编码特征仍不清楚。对内部转录间隔区（ITS）的分析表明，S. segobiensis DSM27193 与 Pichia stipitis（已更名为 Scheffersomyces stipitis）亲缘关系密切，两者都表现出 CTG 编码特征。本研究利用 CodonW、CUPS 等软件工具分析了 S. segobiensis DSM27193 基因组的密码子偏好。此外，我们还通过绿色荧光蛋白（GFP）的表达验证了密码子表的可靠性。分析结果为提高外源基因在 S. segobiensis DSM27193 中的表达提供了理论依据。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.