{"title":"Genetic diversity assessment of Chlorella sp. strains collected from Southern Vietnam","authors":"Tam Minh Phan , Biet Van Huynh","doi":"10.1016/j.scowo.2025.100052","DOIUrl":null,"url":null,"abstract":"<div><div>The southern region of Vietnam, with its distinct river systems and climate, provides a favorable environment for <em>Chlorella</em> sp. growth and development. This study assessed the genetic diversity of eight <em>Chlorella</em> samples using ISSR (Inter-Simple Sequence Repeat) molecular markers. Fourteen ISSR primers generated 184 amplified products, of which 180 were polymorphic, with band sizes ranging from 200 to 4000 bp. The average number of bands per primer was 13.14, with ISSR15 exhibiting the highest polymorphism (100 %) and ISSR2 the lowest (83.3 %). Genetic similarity coefficients ranged from 0.5172 to 0.8152, indicating substantial genetic variation. Phylogenetic analysis grouped the samples into four distinct clusters, with an average genetic similarity of 0.6089. These findings confirm the effectiveness of ISSR markers in assessing genetic diversity in <em>Chlorella</em> and provide insights into its genetic structure and biodiversity in southern Vietnam.</div></div>","PeriodicalId":101197,"journal":{"name":"Sustainable Chemistry One World","volume":"6 ","pages":"Article 100052"},"PeriodicalIF":0.0000,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sustainable Chemistry One World","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2950357425000095","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/2/24 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
The southern region of Vietnam, with its distinct river systems and climate, provides a favorable environment for Chlorella sp. growth and development. This study assessed the genetic diversity of eight Chlorella samples using ISSR (Inter-Simple Sequence Repeat) molecular markers. Fourteen ISSR primers generated 184 amplified products, of which 180 were polymorphic, with band sizes ranging from 200 to 4000 bp. The average number of bands per primer was 13.14, with ISSR15 exhibiting the highest polymorphism (100 %) and ISSR2 the lowest (83.3 %). Genetic similarity coefficients ranged from 0.5172 to 0.8152, indicating substantial genetic variation. Phylogenetic analysis grouped the samples into four distinct clusters, with an average genetic similarity of 0.6089. These findings confirm the effectiveness of ISSR markers in assessing genetic diversity in Chlorella and provide insights into its genetic structure and biodiversity in southern Vietnam.
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