{"title":"Differential gene expression and metabolic pathways in Toona sinensis: Influence on colour and aroma.","authors":"Rui Zeng, Mingmin Zheng, Yunhong Gao, Jianchun Hu, Javed Ahmad, Muhammad Umer Farooq, Songqing Liu, Xiangmei Lin, Suleyman I Allakhverdiev, Sergey Shabala","doi":"10.1111/ppl.70146","DOIUrl":null,"url":null,"abstract":"<p><p>Toona sinensis, a plant species renowned for its culinary and medicinal properties, exhibits diverse colour variations that contribute to its aesthetic appeal and commercial value. Understanding the molecular mechanisms underlying colour and aroma traits in Toona sinensis is crucial for breeding programs and quality regulation in agriculture and the food industry. The present investigation included a comprehensive analysis of the transcriptomic and metabolomic profiles of Toona sinensis with different colours, including green, red, and red leaves with green stems. Metabolic analysis revealed that the flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include terpenoids (5), heterocyclic compounds (1), phenol (1), ketone (1), aldehyde (1), and alcohol (1). Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Functional annotation and pathway analysis revealed that terpene metabolites are predominantly synthesized via terpene metabolic pathway, involving eight key gene families. This study underscores the importance of multi-omics approaches in unravelling the genetic and metabolic basis of phenotypic traits in plant species aimed at improving colour, aroma, and nutritional quality in plants and derived products. HIGHLIGHTS: Flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include five terpenoids, one heterocyclic compound, one phenol, one ketone, one aldehyde, and one alcohol. Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Terpene metabolites are predominantly synthesized via the terpene metabolic pathway, involving eight key gene families. The net photosynthetic rate and intercellular CO<sub>2</sub> concentration are relatively high in the red Toon sinensis morph.</p>","PeriodicalId":20164,"journal":{"name":"Physiologia plantarum","volume":"177 2","pages":"e70146"},"PeriodicalIF":5.4000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11896931/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiologia plantarum","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/ppl.70146","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Toona sinensis, a plant species renowned for its culinary and medicinal properties, exhibits diverse colour variations that contribute to its aesthetic appeal and commercial value. Understanding the molecular mechanisms underlying colour and aroma traits in Toona sinensis is crucial for breeding programs and quality regulation in agriculture and the food industry. The present investigation included a comprehensive analysis of the transcriptomic and metabolomic profiles of Toona sinensis with different colours, including green, red, and red leaves with green stems. Metabolic analysis revealed that the flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include terpenoids (5), heterocyclic compounds (1), phenol (1), ketone (1), aldehyde (1), and alcohol (1). Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Functional annotation and pathway analysis revealed that terpene metabolites are predominantly synthesized via terpene metabolic pathway, involving eight key gene families. This study underscores the importance of multi-omics approaches in unravelling the genetic and metabolic basis of phenotypic traits in plant species aimed at improving colour, aroma, and nutritional quality in plants and derived products. HIGHLIGHTS: Flavonoid biosynthesis pathway governs the colour distinction between green and red Toona sinensis. The top 10 metabolites influenced by transcriptome include five terpenoids, one heterocyclic compound, one phenol, one ketone, one aldehyde, and one alcohol. Fifteen highly expressed genes impacted by phenylpropanoid, sesquiterpenoid, and triterpenoid biosynthesis in coloured Toona sinensis. Terpene metabolites are predominantly synthesized via the terpene metabolic pathway, involving eight key gene families. The net photosynthetic rate and intercellular CO2 concentration are relatively high in the red Toon sinensis morph.
期刊介绍:
Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.