Microbial Depletion Is Associated with Slower Cnidarian Regeneration.

Abstract

Microbiomes play an important role in physiology and development in cnidarians, but how these communities influence tissue regeneration is poorly understood. Here, we examined the effects of antibiotic exposure on regeneration and microbial communities in two cnidarian models, the sea anemones Nematostella vectensis (non-symbiotic, hereafter, Nematostella) and Exaiptasia diaphana (symbiotic, hereafter, Aiptasia). Bisected animals were incubated in either sterile or antibiotic-treated artificial seawater for 7 days and regeneration was monitored daily. After 7 days, tentacle number and length were measured, and microbial communities were profiled using metabarcoding of the V4 region of the 16S rRNA. Microbiome disruption was observed under antibiotic treatment in both species, resulting in decreased microbial load and shifts in relative abundances of certain microbial taxa. Nematostella exhibited a greater reduction in microbial diversity and community shifts under antibiotic exposure, whereas Aiptasia showed only moderate changes in diversity. In both species, microbiome disruption was associated with slower regeneration rates and reduced tentacle number and length, suggesting a functional role for the microbiome in anemone regeneration. Our findings suggest that host-microbiome interactions in both symbiotic and aposymbiotic anemones are important for the maintenance of regenerative processes. These findings provide insight into how cnidarians and their microbiomes respond to environmental stressors, with implications for predicting cnidarian resilience in the context of emerging threats to the marine environment.