Variety, maturity stage and postharvest storage time effect on Mal d 3 allergen from apple (Malus domestica)

IF 6.4 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2025-03-13 DOI:10.1016/j.postharvbio.2025.113512

Clara Esteban-Sanz , Ana P. Tobajas , Ana P. Gracia, Alba Civera, Lourdes Sánchez, Esther Arias, María D. Pérez

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Abstract

Fruit belonging to Rosaceae family, which includes apples, is responsible for 60 % of fruit allergies. Mal d 3, a member of the lipid transfer proteins, is the most allergenic protein of apple. Levels of total soluble protein and Mal d 3 were determined in apple peel as a function of variety, maturity stage and postharvest storage time under controlled atmosphere conditions, using a previously developed specific sandwich ELISA. Mal d 3 content differed among varieties, ranging from 13.6 mg kg⁻¹ in ‘Golden Delicious’ to 23.6 mg kg⁻¹ in ‘Verde Doncella’. No significant influence of the maturity stage on Mal d 3 concentration was found. Over a 7-month period of storage under controlled atmosphere, the levels of Mal d 3 decreased about 1.6–2.3 times. No correlation was found between Mal d 3 concentration and total soluble protein in apple peel samples. A better understanding of how Mal d 3 concentration is affected by pre and postharvest factors could assist to decrease the allergen load of apple.

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.