Variety, maturity stage and postharvest storage time effect on Mal d 3 allergen from apple (Malus domestica)

IF 6.8 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2025-03-13 DOI:10.1016/j.postharvbio.2025.113512

Clara Esteban-Sanz , Ana P. Tobajas , Ana P. Gracia, Alba Civera, Lourdes Sánchez, Esther Arias, María D. Pérez

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Abstract

Fruit belonging to Rosaceae family, which includes apples, is responsible for 60 % of fruit allergies. Mal d 3, a member of the lipid transfer proteins, is the most allergenic protein of apple. Levels of total soluble protein and Mal d 3 were determined in apple peel as a function of variety, maturity stage and postharvest storage time under controlled atmosphere conditions, using a previously developed specific sandwich ELISA. Mal d 3 content differed among varieties, ranging from 13.6 mg kg⁻¹ in ‘Golden Delicious’ to 23.6 mg kg⁻¹ in ‘Verde Doncella’. No significant influence of the maturity stage on Mal d 3 concentration was found. Over a 7-month period of storage under controlled atmosphere, the levels of Mal d 3 decreased about 1.6–2.3 times. No correlation was found between Mal d 3 concentration and total soluble protein in apple peel samples. A better understanding of how Mal d 3 concentration is affected by pre and postharvest factors could assist to decrease the allergen load of apple.

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品种、成熟期和采后贮藏时间对苹果（Malus domestica） Mal d 3过敏原的影响

蔷薇科的水果，包括苹果，导致60% %的水果过敏。脂质转移蛋白成员Mal d3是苹果最易致敏的蛋白。利用之前开发的特异性夹心酶联免疫吸附试验（sandwich ELISA），测定了苹果皮中总可溶性蛋白（total soluble protein）和Mal d - 3含量与品种、成熟期和采后贮藏时间的关系。不同品种间Mal d - 3含量差异较大，‘Golden Delicious’为13.6 mg kg - 1，‘Verde Doncella’为23.6 mg kg - 1。成熟期对Mal d - 3浓度无显著影响。在可控气氛下贮藏7个月后，Mal d 3的含量降低了1.6 ~ 2.3 倍。苹果皮中Mal d 3浓度与总可溶性蛋白之间无相关性。更好地了解采前和采后因素对Mal - 3浓度的影响，有助于减少苹果的过敏原负荷。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.