Transcriptomic and metabolic profiling reveals the effects of long-term microwave exposure on testicular tissue

Abstract

The effect of electromagnetic exposure on health is becoming increasingly important as it affects many aspects of human life and health. However, the effects in environmental electromagnetic fields on the male reproductive system were still controversial, and the impacts of long-term microwave exposure on testicular tissue remain poorly defined. This study exposed rats to 30 mW/cm² of microwave radiation (2.856 GHz) for six weeks and revealed that long-term microwave exposure damaged the testis structures, sperm motility, and morphology, affected hormone levels, energy metabolism, and induced oxidative stress. Assays for bulk RNA, metabonomics, single-cell RNA, and transposase-accessible chromatin with high-throughput sequencing were performed to analyze the transcriptional and metabolic atlas of testicular damage after microwave radiation. Differentially expressed genes were enriched in oxidative stress and energy metabolism pathways. Furthermore, ten subgroups were identified with scRNA-seq, including five developmental phases of germ cells, and radiation-associated changes in cell composition, especially stuck in round spermatids, were observed. Radiation significantly upregulated the expression of Atp6v1e2 in round spermatids and enriched the expression of many transcription factors by disturbing the accessibility profile of chromatin. This study provides effective insights into the long-term impacts of microwave radiation on male reproduction.