Characterization and performance of efficient heterotrophic nitrification and aerobic denitrification by Comamonas testosteroni HR5 under low temperature and high alkalinity

IF 6.3 2区工程技术 Q1 ENGINEERING, CHEMICAL Journal of water process engineering Pub Date : 2025-03-13 DOI:10.1016/j.jwpe.2025.107474

Rui Huo, Wanying Li, Yiling Di, Shilei Zhou

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引用次数: 0

Abstract

A newly isolated Comamonas testosteroni strain, HR5, exhibited efficient nitrogen removal capacities at low temperature and high alkalinity. HR5 efficiently removed nitrate nitrogen (removal efficiency ≥95 %) at C/N = 12–15, pH 6–11, and 5–25 °C. Furthermore, the removal efficiency of total dissolved nitrogen (TDN) for ammonia, nitrate, and nitrite as the sole nitrogen source system reached 97.90 ± 0.02 %, 95.16 ± 0.01 %, and 99.95 ± 0.00 %, respectively; the removal efficiency for the mixed nitrogen system was >91 % at 5 °C and pH 10. Nitrogen balance indicated that HR5 converted initial nitrogen into gaseous products (20.87–77.13 %) and biological nitrogen (19.58–75.65 %), with the percentage of gaseous N increasing as the temperature increased. Furthermore, typical cold-resistance genes (cspA, infB, and rbfA) and alkalinity-resistance genes (Pha and Trk) were involved. These results provide a reference for practical applications of Comamonas testosteroni that involve low temperature and high alkalinity.

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来源期刊

Journal of water process engineering Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

10.70

自引率

8.60%

发文量

846

审稿时长

24 days

期刊介绍： The Journal of Water Process Engineering aims to publish refereed, high-quality research papers with significant novelty and impact in all areas of the engineering of water and wastewater processing . Papers on advanced and novel treatment processes and technologies are particularly welcome. The Journal considers papers in areas such as nanotechnology and biotechnology applications in water, novel oxidation and separation processes, membrane processes (except those for desalination) , catalytic processes for the removal of water contaminants, sustainable processes, water reuse and recycling, water use and wastewater minimization, integrated/hybrid technology, process modeling of water treatment and novel treatment processes. Submissions on the subject of adsorbents, including standard measurements of adsorption kinetics and equilibrium will only be considered if there is a genuine case for novelty and contribution, for example highly novel, sustainable adsorbents and their use: papers on activated carbon-type materials derived from natural matter, or surfactant-modified clays and related minerals, would not fulfil this criterion. The Journal particularly welcomes contributions involving environmentally, economically and socially sustainable technology for water treatment, including those which are energy-efficient, with minimal or no chemical consumption, and capable of water recycling and reuse that minimizes the direct disposal of wastewater to the aquatic environment. Papers that describe novel ideas for solving issues related to water quality and availability are also welcome, as are those that show the transfer of techniques from other disciplines. The Journal will consider papers dealing with processes for various water matrices including drinking water (except desalination), domestic, urban and industrial wastewaters, in addition to their residues. It is expected that the journal will be of particular relevance to chemical and process engineers working in the field. The Journal welcomes Full Text papers, Short Communications, State-of-the-Art Reviews and Letters to Editors and Case Studies