Characterization and performance of efficient heterotrophic nitrification and aerobic denitrification by Comamonas testosteroni HR5 under low temperature and high alkalinity
{"title":"Characterization and performance of efficient heterotrophic nitrification and aerobic denitrification by Comamonas testosteroni HR5 under low temperature and high alkalinity","authors":"Rui Huo, Wanying Li, Yiling Di, Shilei Zhou","doi":"10.1016/j.jwpe.2025.107474","DOIUrl":null,"url":null,"abstract":"<div><div>A newly isolated <em>Comamonas testosteroni</em> strain, HR5, exhibited efficient nitrogen removal capacities at low temperature and high alkalinity. HR5 efficiently removed nitrate nitrogen (removal efficiency ≥95 %) at C/<em>N</em> = 12–15, pH 6–11, and 5–25 °C. Furthermore, the removal efficiency of total dissolved nitrogen (TDN) for ammonia, nitrate, and nitrite as the sole nitrogen source system reached 97.90 ± 0.02 %, 95.16 ± 0.01 %, and 99.95 ± 0.00 %, respectively; the removal efficiency for the mixed nitrogen system was >91 % at 5 °C and pH 10. Nitrogen balance indicated that HR5 converted initial nitrogen into gaseous products (20.87–77.13 %) and biological nitrogen (19.58–75.65 %), with the percentage of gaseous N increasing as the temperature increased. Furthermore, typical cold-resistance genes (<em>cspA</em>, <em>infB</em>, and <em>rbfA</em>) and alkalinity-resistance genes (<em>Pha</em> and <em>Trk</em>) were involved. These results provide a reference for practical applications of <em>Comamonas testosteroni</em> that involve low temperature and high alkalinity.</div></div>","PeriodicalId":17528,"journal":{"name":"Journal of water process engineering","volume":"72 ","pages":"Article 107474"},"PeriodicalIF":6.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of water process engineering","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221471442500546X","RegionNum":2,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/13 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
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Abstract
A newly isolated Comamonas testosteroni strain, HR5, exhibited efficient nitrogen removal capacities at low temperature and high alkalinity. HR5 efficiently removed nitrate nitrogen (removal efficiency ≥95 %) at C/N = 12–15, pH 6–11, and 5–25 °C. Furthermore, the removal efficiency of total dissolved nitrogen (TDN) for ammonia, nitrate, and nitrite as the sole nitrogen source system reached 97.90 ± 0.02 %, 95.16 ± 0.01 %, and 99.95 ± 0.00 %, respectively; the removal efficiency for the mixed nitrogen system was >91 % at 5 °C and pH 10. Nitrogen balance indicated that HR5 converted initial nitrogen into gaseous products (20.87–77.13 %) and biological nitrogen (19.58–75.65 %), with the percentage of gaseous N increasing as the temperature increased. Furthermore, typical cold-resistance genes (cspA, infB, and rbfA) and alkalinity-resistance genes (Pha and Trk) were involved. These results provide a reference for practical applications of Comamonas testosteroni that involve low temperature and high alkalinity.
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