Surface engineering of zirconia with abaloparatide and nano-hydroxyapatite for synergistic osteogenic activity

IF 3.4 4区工程技术 Q2 POLYMER SCIENCE Macromolecular Research Pub Date : 2024-10-24 DOI:10.1007/s13233-024-00336-9

Hong Jae Lee, Yong-Dae Kwon, Sang Cheon Lee

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Abstract

We report on the surface engineering of zirconia (ZrO₂) by incorporation of abaloparatide (ABL) on the surface of nano-hydroxyapatite (nHAp)-immobilized zirconia. First, nHAp-immobilized zirconia (nHAp–PDA–ZrO₂) was fabricated by adding nHAp in a polydopamine (PDA)-coating process on zirconia surface. Surface chemistry, morphology, and wettability of nHAp–PDA–ZrO₂ were examined using X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), and water contact angle measurement. As a second step, ABL incorporation was performed by the reaction of ABL with PDA layer coated on nHAp–PDA–ZrO₂. A fluorescence microscope visualized that ABL was efficiently bound on the surface of nHAp–PDA–ZrO₂to form ABL/nHAp–PDA–ZrO₂. The synergistic osteogenic effect of ABL and nHAP on alkaline phosphatase (ALP) activity, calcium-mineral deposition, and alizarin red staining were assessed. The combination of ABL and nHAp for surface-engineering approaches may provide an effective approach to develop highly promising bone-regenerative osteogenic implant surfaces.

Graphical abstract

The surface of zirconia is engineered by incorporation of bone-forming abaloparatide (ABL) and osteoconductive nano-hydroxyapatite (nHAp). This ABL/nHAp-functionalized zirconia surface exhibits synergistic improved osteogenic activity.

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氧化锆与阿巴巴拉肽和纳米羟基磷灰石的表面工程协同成骨活性

本文报道了在纳米羟基磷灰石(nHAp)-固定化氧化锆表面掺入abaloparatide （ABL）的氧化锆（ZrO2）表面工程。首先，采用聚多巴胺（PDA）包覆法在氧化锆表面添加nHAp制备了nHAp- PDA - zro2固定氧化锆（nHAp - PDA - zro2）。采用x射线光电子能谱（XPS）、场发射扫描电镜（FE-SEM）和水接触角测量对nHAp-PDA-ZrO2的表面化学、形貌和润湿性进行了检测。第二步，ABL与包覆在nHAp-PDA-ZrO2上的PDA层反应，实现ABL的掺入。荧光显微镜观察到ABL在nHAp-PDA-ZrO2表面有效结合形成ABL/ nHAp-PDA-ZrO2。观察ABL和nHAP对碱性磷酸酶（ALP）活性、钙矿物沉积和茜素红染色的协同成骨作用。ABL和nHAp结合用于表面工程方法可能为开发极具前景的骨再生成骨种植体表面提供有效途径。图示：氧化锆的表面是由骨形成的abaloparatide （ABL）和骨导电性纳米羟基磷灰石（nHAp）结合而成。这种ABL/ nhap功能化的氧化锆表面表现出协同改善的成骨活性。

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来源期刊

Macromolecular Research 工程技术-高分子科学

CiteScore

4.70

自引率

8.30%

发文量

100

审稿时长

1.3 months

期刊介绍： Original research on all aspects of polymer science, engineering and technology, including nanotechnology Presents original research articles on all aspects of polymer science, engineering and technology Coverage extends to such topics as nanotechnology, biotechnology and information technology The English-language journal of the Polymer Society of Korea Macromolecular Research is a scientific journal published monthly by the Polymer Society of Korea. Macromolecular Research publishes original researches on all aspects of polymer science, engineering, and technology as well as new emerging technologies using polymeric materials including nanotechnology, biotechnology, and information technology in forms of Articles, Communications, Notes, Reviews, and Feature articles.