StZIP2 promotes root growth by improving the transport efficiency of zinc in potato(Solanum tuberosum L.).

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2025-03-01 DOI:10.1111/ppl.70153
Junliang Fan, Cunlan Zhu, Xingru Si, Wenjin Xu, Liang Yang, Kaitong Wang, Ning Zhang, Huaijun Si
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Abstract

Zinc is an essential trace element for plant growth and development. Zinc transporters play an important role in regulating zinc homeostasis in plants. In this study, the potato cultivar 'Atlantic' was used as experimental material to analyze the expression characteristics of the StZIP2 gene in different potato tissues under zinc deficiency stress. Transgenic plants with overexpression and interference expression of the StZIP2 gene were obtained by genetic transformation and treated with zinc deficiency stress. Chlorophyll content, antioxidant enzyme activity, proline (Pro) and malonic dialdehyde (MDA) content, zinc content in aboveground parts and roots, and root indices were determined. The results showed that the expression level of the StZIP2 gene in roots, stems and leaves under zinc deficiency stress was significantly higher than that of the control, and the expression level of the StZIP2 gene in roots under zinc deficiency stress was the highest. After zinc deficiency treatment, the content of chlorophyll and Pro, the activity of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), root-to-shoot ratio, root length and root fresh weight of overexpressed plants were significantly increased, while the MDA content was significantly decreased. The ratio of zinc content between the above-ground part and the root of the overexpressed plants was significantly higher than that of the non-transgenic plants, and the transport efficiency from the root to the above-ground part was significantly increased in the overexpressed plants, whereas it was just the opposite in the interference expressing plants. The result provides basic data to further elucidate the StZIP2 gene function.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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