Atypical sulfur-containing physalin from Physalis minima and protective effect against ischemia-reperfusion injury

IF 3.2 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Phytochemistry Pub Date : 2025-03-12 DOI:10.1016/j.phytochem.2025.114478
Jiangping Wu , Zixu Li , Jianping Zhao , Tao Zhang , Jun Zhang , Qiuyue Lv , Jiangfei Li , Guodong Wang , Jun Han , Zhongmei Zou
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Abstract

Four previously undescribed physalins (14), along with six known ones (510) were isolated and identified from the whole plants of Physalis minima L., a medicinal and edible plant traditionally used in southwest China. Their structures were established through comprehensive spectroscopic analyses, including high-resolution electrospray ionization mass spectrometry and 1D/2D nuclear magnetic resonance spectroscopy. Moreover, the absolute configurations of 13, 5 and 7 were examined by X-ray diffraction analyses. Compound 1, an undescribed sulfur-containing physalin, exhibited the most protective effect against oxygen-glucose deprivation/reperfusion (OGD/R)-stimulated ischemia-reperfusion (I/R) injury in PC12 cells. Meanwhile, compound 1 was found to reduce the inflammatory response, with mechanistic studies indicating that it decreased pyroptosis-associated proteins, such as cleaved-caspase1, NLRP3, and GSDMD N-terminus. Importantly, GSDMD knockdown significantly reversed the protective effects of compound 1, highlighting the involvement of pyroptosis in the compound's protective mechanism against OGD/R-induced I/R injury in PC12 cells in vitro.

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从中国西南地区传统的药用和食用植物 Physalis minima L. 的全株中分离并鉴定了四种以前未曾描述过的 Physalins(1-4)和六种已知的 Physalins(5-10)。通过高分辨率电喷雾电离质谱和一维/二维核磁共振波谱等综合光谱分析,确定了它们的结构。此外,还通过 X 射线衍射分析研究了 1-3、5 和 7 的绝对构型。化合物 1 是一种未曾描述过的含硫物理苷,对 PC12 细胞在氧-葡萄糖剥夺/再灌注(OGD/R)刺激下的缺血再灌注损伤具有最强的保护作用。同时,化合物 1 还能减轻炎症反应,其机理研究表明,它能减少裂解-caspase1、NLRP3 和 GSDMD N-terminus 等热蛋白相关蛋白。重要的是,敲除 GSDMD 会显著逆转化合物 1 的保护作用,这突出表明了该化合物在体外 PC12 细胞中对 OGD/R 诱导的 I/R 损伤的保护机制中参与了热蛋白沉积。
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来源期刊
Phytochemistry
Phytochemistry 生物-植物科学
CiteScore
6.40
自引率
7.90%
发文量
443
审稿时长
39 days
期刊介绍: Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.
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