Fan Liu , Yunpeng Wang , Yijie Wang , Jingwen Zhou , Jianghua Li , Jian Chen , Guocheng Du , Xinrui Zhao
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引用次数: 0
Abstract
The biosynthesis of multi-subunit hemoglobins that can be used in artificial oxygen carriers, artificial foods and medical diagnostics has become a hot research issue. However, the imbalanced expression of different subunits and insufficient heme-supply limit the efficient production of multi-subunit hemoglobins. After the equalized synthesis of different globins through the regulation at transcriptional and translational levels, the improvement of heme-supply by relieving the feedback inhibition of heme on Hem1p and the enhancement of key rate-limiting enzymes in yeast, a robust platform for the expression of high-active hemoglobins was constructed. The synthesis of hemoglobins with high-activity were achieved, including human hemoglobin (174.2 mg/L, 61.7 % mol heme/mol Hb), porcine hemoglobin (203.9 mg/L, 72.1 % mol heme/mol Hb) and bovine hemoglobin (258.2 mg/L, 64.9 % mol heme/mol Hb). Therefore, the applied strategies can be broadly used in the production of other heteromultimeric and heme-binding proteins.
期刊介绍:
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.