Recombinant α-Toxin BmK-M9 Inhibits Breast Cancer Progression by Regulating β-Catenin In Vivo

IF 2.5 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Cell Biochemistry and Biophysics Pub Date : 2025-03-13 DOI:10.1007/s12013-025-01711-8

Wenlin Chen, Zhuocen Cha, Saijun Huang, Ruimin Liu, Jiayi Chen, Peter Muiruri Kamau, Xingjia Lu, Bowen Li, Dequan Liu

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Abstract

Screening bioactive compounds from natural sources, including animals and plants, is a valuable strategy for identifying novel anti-tumor agents. α-Toxin BmK-M9, a key component of scorpion venom, has received limited attention regarding its potential anti-cancer effects and underlying mechanisms in breast cancer. This study investigates the effects and mechanisms of BmK-M9 in breast cancer using in vitro experiments and a nude mouse model. mRNA sequencing was performed to identify affected signaling pathways, while Western blotting and immunohistochemistry were utilized to analyze the Wnt/β-catenin signaling pathway. The results demonstrated that BmK-M9 significantly inhibited breast cancer cell invasion and migration in vitro and suppressed tumor growth in vivo. Transcriptomic analysis revealed that BmK-M9 influenced cellular processes related to proliferation, apoptosis, motility, and metabolism. Furthermore, BmK-M9 markedly downregulated β-catenin expression in the Wnt/β-catenin pathway. These findings suggest that BmK-M9 exerts anti-tumor effects in breast cancer by modulating Wnt/β-catenin signaling, highlighting its potential as a promising therapeutic candidate.

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体内重组α-毒素BmK-M9通过调节β-Catenin抑制乳腺癌进展

从天然来源（包括动物和植物）中筛选生物活性化合物是鉴定新型抗肿瘤药物的一种有价值的策略。α-毒素BmK-M9是蝎毒液的一种重要成分，其潜在的抗癌作用及其在乳腺癌中的作用机制目前受到的关注较少。本研究通过体外实验和裸鼠模型探讨BmK-M9在乳腺癌中的作用及其机制。mRNA测序鉴定受影响的信号通路，Western blotting和免疫组织化学分析Wnt/β-catenin信号通路。结果表明，BmK-M9在体外显著抑制乳腺癌细胞的侵袭和迁移，在体内显著抑制肿瘤生长。转录组学分析显示BmK-M9影响与增殖、凋亡、运动和代谢相关的细胞过程。此外，BmK-M9在Wnt/β-catenin通路中显著下调β-catenin的表达。这些发现表明BmK-M9通过调节Wnt/β-catenin信号传导在乳腺癌中发挥抗肿瘤作用，突出了其作为一种有前景的治疗候选药物的潜力。

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来源期刊

Cell Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

7.5 months

期刊介绍： Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.