Statistical Optimization of Exopolysaccharide and Biomass Production by Mangrove Fungi Fusarium equiseti ANP2 and its Potential Application as Bioemulsifier and Chelator.

IF 2.3 3区 生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2025-03-13 DOI:10.1007/s00284-025-04129-w
A M V N Prathyusha, Pallaval Veera Bramhachari
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引用次数: 0

Abstract

The rationale of the study is to explore the bio functional industrial potential and optimized culture conditions of a Manno glucan heteropolysaccharide MF-1 (purified EPS fraction) produced by a newly discovered mangrove derived fungi Fusarium equiseti ANP2, isolated from the Krishna River delta mangrove sediments. Response surface methodology (RSM) was employed to optimize fungal EPS and Biomass production, achieving a significant 1.4-fold increase to 6.94 g/L in EPS yield and a 2.1-fold increase in biomass production. RSM identified optimal levels of glucose, NH₄NO₃, NaCl, leucine, temperature, and pH, while minimizing the required glucose and nitrogen content compared to conventional methods. Notably, MF-1 exhibited promising emulsification potential (69.5% n-hexadecane emulsification), suggesting its prospective role as a novel emulsifier, particularly for n-hexadecane-based applications. Additionally, MF-1 also displayed a chelating activity for Fe2⁺ ions, suggesting its applicability as a natural chelating agent. The current study optimized the EPS production using RSM design and explored its potential for industrial applications as emulsification and chelating properties of the purified EPS fraction. Future research could explore the structural modifications of the fungal EPS to enhance its functionalities and delve deeper into the mechanisms governing EPS and biomass for large-scale, sustainable industrial production.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
期刊最新文献
Elucidating the Microsatellite Signature of the Tri-Partite Genomes of the Viral Family Peribunyaviridae. Genetic Diversity in Antimicrobial Resistance Determinants Among Pathogenic Pseudomonas aeruginosa in India. Statistical Optimization of Exopolysaccharide and Biomass Production by Mangrove Fungi Fusarium equiseti ANP2 and its Potential Application as Bioemulsifier and Chelator. Synergistic Antimicrobial Effects of Melaleuca alternifolia Essential Oil and Kojic Acid Combinations. Description of Cytobacillus Mangrovibacter sp. nov., and Cytobacillus Spartinae sp. nov., Isolated from Mangrove Sediment.
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