Draft genome of the multidrug-resistant Citrobacter freundii strain CAPA023 isolated from Arapaima gigas in Peru: is it a reservoir of resistance and virulence genes?
Enrique Garcia-Candela, Aaron Mondragón-Martínez, Manuel Noceda-Rodríguez, Fernando Mesias Valle, Milagros Cabrera-Soregui, Veronica Valverde-Vera, Maria Benito-García, Romina Romayna-Ríos, Miriam Verástegui-Tello, Saurabh Dubey, Hetron M Munang'andu, Jefferson Yunis-Aguinaga
{"title":"Draft genome of the multidrug-resistant Citrobacter freundii strain CAPA023 isolated from Arapaima gigas in Peru: is it a reservoir of resistance and virulence genes?","authors":"Enrique Garcia-Candela, Aaron Mondragón-Martínez, Manuel Noceda-Rodríguez, Fernando Mesias Valle, Milagros Cabrera-Soregui, Veronica Valverde-Vera, Maria Benito-García, Romina Romayna-Ríos, Miriam Verástegui-Tello, Saurabh Dubey, Hetron M Munang'andu, Jefferson Yunis-Aguinaga","doi":"10.1016/j.jgar.2025.03.005","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>The purpose of this study was to analyze the genome of the multidrug-resistant Citrobacter freundii strain CAPA023, which was obtained from diseased Arapaima gigas fry. The study focused on determining mobile genetic elements and genetic factors that contribute to antibiotic resistance and pathogenicity.</p><p><strong>Methods: </strong>Genomic DNA was sequenced using Illumina NovaSeq (2 × 150 bp) and assembled de novo using Shovill v1.1.0. Resistance genes, virulence factors, plasmids, and mobile elements were identified using ResFinder, CARD, PlasmidFinder, MobileFinder, PathogenFinder, and VFDB.</p><p><strong>Results: </strong>The 5,059,550 bp draft genome (60 contigs, 51.5% GC) revealed resistance genes for various antibiotic classes, efflux pumps, IncFIB(K) and Col440I plasmids, insertion sequences, and multiple virulence genes.</p><p><strong>Conclusion: </strong>Considering that this bacterium was found in diseased fish, it is possible that C. freundii plays an important role in the spread of virulence factors and antibiotic resistance in aquaculture environments. This highlights the importance of genomic surveillance in Amazonian aquaculture.</p>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":" ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.jgar.2025.03.005","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: The purpose of this study was to analyze the genome of the multidrug-resistant Citrobacter freundii strain CAPA023, which was obtained from diseased Arapaima gigas fry. The study focused on determining mobile genetic elements and genetic factors that contribute to antibiotic resistance and pathogenicity.
Methods: Genomic DNA was sequenced using Illumina NovaSeq (2 × 150 bp) and assembled de novo using Shovill v1.1.0. Resistance genes, virulence factors, plasmids, and mobile elements were identified using ResFinder, CARD, PlasmidFinder, MobileFinder, PathogenFinder, and VFDB.
Results: The 5,059,550 bp draft genome (60 contigs, 51.5% GC) revealed resistance genes for various antibiotic classes, efflux pumps, IncFIB(K) and Col440I plasmids, insertion sequences, and multiple virulence genes.
Conclusion: Considering that this bacterium was found in diseased fish, it is possible that C. freundii plays an important role in the spread of virulence factors and antibiotic resistance in aquaculture environments. This highlights the importance of genomic surveillance in Amazonian aquaculture.
期刊介绍：
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
