A TaSnRK1α-TaCAT2 model mediates resistance to Fusarium crown rot by scavenging ROS in common wheat

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES Nature Communications Pub Date : 2025-03-15 DOI:10.1038/s41467-025-57936-x

Xia Yang, Leilei Zhang, Jiajie Wei, Lexin Liu, Di Liu, Xiangning Yan, Minjie Yuan, Lingran Zhang, Ning Zhang, Yan Ren, Feng Chen

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Abstract

Fusarium crown rot (FCR) is a serious underlying disease to threaten wheat yield and quality recently. Here, we identify a catalase antioxidant enzyme (TaCAT2) through genome wide association study (GWAS) and whole-exome sequencing (WES) in two nested bi-parental populations. We verify the function of TaCAT2 regulating wheat FCR resistance by genetic transformation. Moreover, we screen a sucrose non-fermenting-1-related protein kinase alpha subunit (TaSnRK1α) interacting with TaCAT2, and subsequently find that TaSnRK1α phosphorylates TaCAT2. We next identify an FCR-resistance haplotype TaCAT2^Ser214, and confirm that Ser214 of TaCAT2 is a key phosphorylation site for TaSnRK1α. We also find that TaSnRK1α results in higher protein accumulation in TaCAT2^Ser214 than in TaCAT2^Thr214, which possibly contribute to scavenging ROS (reactive oxygen species) in TaCAT2^Ser214 wheat plants. Furthermore, the function of TaSnRK1α regulating FCR resistance is verified by genetic transformation. Taken together, we propose a TaSnRK1α-TaCAT2 model to mediate FCR resistance by scavenging the ROS in wheat plants.

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一个TaSnRK1α-TaCAT2模型通过清除活性氧介导小麦对枯萎病的抗性

镰刀菌冠腐病是近年来严重威胁小麦产量和品质的潜在病害。在这里，我们通过基因组全关联研究（GWAS）和全外显子组测序（WES）在两个嵌套的双亲本群体中鉴定了过氧化氢酶抗氧化酶（TaCAT2）。我们通过遗传转化验证了TaCAT2调控小麦FCR抗性的功能。此外，我们筛选了一个与TaCAT2相互作用的蔗糖非发酵1相关蛋白激酶α亚基（TaSnRK1α），随后发现TaSnRK1α使TaCAT2磷酸化。接下来，我们确定了一个耐fcr单倍型TaCAT2Ser214，并证实TaCAT2的Ser214是TaSnRK1α的关键磷酸化位点。我们还发现TaSnRK1α在TaCAT2Ser214中比在TaCAT2Thr214中导致更高的蛋白质积累，这可能有助于清除TaCAT2Ser214小麦植株中的活性氧（ROS）。此外，通过基因转化证实了TaSnRK1α调控FCR抗性的功能。综上所述，我们提出了一个TaSnRK1α-TaCAT2模型，通过清除小麦植株的ROS来介导FCR抗性。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.