Effects of Octacosanol Isolated from Moringa oleifera Leaves on Inhibiting the Activity of Pancreatic Lipase

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of Agricultural and Food Chemistry Pub Date : 2025-03-15 DOI:10.1021/acs.jafc.4c07563
Xiaoying Dong, Yuxi Wen, Qing Nie, Shuo Shan, Runfan Zhao, Hesham R. El-Seedi, Chao Zhao, Yongzhen Zhai
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Abstract

Moringa oleifera Lam., a perennial species of the Moringaceae family, is esteemed for its multifaceted nutritional, medicinal, and economic properties. M. oleifera leaves are abundant in bioactive compounds with potential health benefits, yet existing structural and functional studies of these bioactives remain insufficiently comprehensive. This study aimed to isolate the active compound from M. oleifera leaves and investigate its mechanism of inhibiting lipid absorption and its potential as a pancreatic lipase inhibitor. In the present study, octacosanol (OCT) was isolated and purified from M. oleifera leaves, demonstrating notable pancreatic lipase inhibitory activity with an IC50 of 7.87 ± 0.72 μg/mL, and effectively suppressing lipid uptake in HepG2 cells. Simulated digestion assays indicated that OCT retained 73.5% of its pancreatic lipase inhibitory activity, with a recorded inhibition rate of 63.62%. Molecular docking analyses revealed that OCT binds to pancreatic lipase with an affinity comparable to orlistat and stronger than that of 4-nitrophenyl palmitate. The binding of OCT to key active sites (Ser152, His263, and Asp176) likely disrupts the enzyme’s conformation, decreasing its substrate affinity. Additionally, OCT significantly attenuated lipid absorption and the synthesis of total cholesterol and triglycerides. This study elucidates the lipid-lowering mechanism of OCT and provides a theoretical foundation for its potential application in food production and biomedicine.

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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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