Xiaoying Dong, Yuxi Wen, Qing Nie, Shuo Shan, Runfan Zhao, Hesham R. El-Seedi, Chao Zhao, Yongzhen Zhai
{"title":"Effects of Octacosanol Isolated from Moringa oleifera Leaves on Inhibiting the Activity of Pancreatic Lipase","authors":"Xiaoying Dong, Yuxi Wen, Qing Nie, Shuo Shan, Runfan Zhao, Hesham R. El-Seedi, Chao Zhao, Yongzhen Zhai","doi":"10.1021/acs.jafc.4c07563","DOIUrl":null,"url":null,"abstract":"<i>Moringa oleifera</i> Lam., a perennial species of the Moringaceae family, is esteemed for its multifaceted nutritional, medicinal, and economic properties. <i>M. oleifera</i> leaves are abundant in bioactive compounds with potential health benefits, yet existing structural and functional studies of these bioactives remain insufficiently comprehensive. This study aimed to isolate the active compound from <i>M. oleifera</i> leaves and investigate its mechanism of inhibiting lipid absorption and its potential as a pancreatic lipase inhibitor. In the present study, octacosanol (OCT) was isolated and purified from <i>M. oleifera</i> leaves, demonstrating notable pancreatic lipase inhibitory activity with an IC<sub>50</sub> of 7.87 ± 0.72 μg/mL, and effectively suppressing lipid uptake in HepG2 cells. Simulated digestion assays indicated that OCT retained 73.5% of its pancreatic lipase inhibitory activity, with a recorded inhibition rate of 63.62%. Molecular docking analyses revealed that OCT binds to pancreatic lipase with an affinity comparable to orlistat and stronger than that of 4-nitrophenyl palmitate. The binding of OCT to key active sites (Ser152, His263, and Asp176) likely disrupts the enzyme’s conformation, decreasing its substrate affinity. Additionally, OCT significantly attenuated lipid absorption and the synthesis of total cholesterol and triglycerides. This study elucidates the lipid-lowering mechanism of OCT and provides a theoretical foundation for its potential application in food production and biomedicine.","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":"33 1","pages":""},"PeriodicalIF":5.7000,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Agricultural and Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1021/acs.jafc.4c07563","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Moringa oleifera Lam., a perennial species of the Moringaceae family, is esteemed for its multifaceted nutritional, medicinal, and economic properties. M. oleifera leaves are abundant in bioactive compounds with potential health benefits, yet existing structural and functional studies of these bioactives remain insufficiently comprehensive. This study aimed to isolate the active compound from M. oleifera leaves and investigate its mechanism of inhibiting lipid absorption and its potential as a pancreatic lipase inhibitor. In the present study, octacosanol (OCT) was isolated and purified from M. oleifera leaves, demonstrating notable pancreatic lipase inhibitory activity with an IC50 of 7.87 ± 0.72 μg/mL, and effectively suppressing lipid uptake in HepG2 cells. Simulated digestion assays indicated that OCT retained 73.5% of its pancreatic lipase inhibitory activity, with a recorded inhibition rate of 63.62%. Molecular docking analyses revealed that OCT binds to pancreatic lipase with an affinity comparable to orlistat and stronger than that of 4-nitrophenyl palmitate. The binding of OCT to key active sites (Ser152, His263, and Asp176) likely disrupts the enzyme’s conformation, decreasing its substrate affinity. Additionally, OCT significantly attenuated lipid absorption and the synthesis of total cholesterol and triglycerides. This study elucidates the lipid-lowering mechanism of OCT and provides a theoretical foundation for its potential application in food production and biomedicine.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.