Resistance development, cross-resistance, and inheritance patterns in clothianidin and triazophos resistant strains of Oxycarenus hyalinipennis

IF 2.5 2区农林科学 Q1 AGRONOMY Crop Protection Pub Date : 2025-03-12 DOI:10.1016/j.cropro.2025.107197

Rabia Saeed , Naeem Abbas , Muhammad Akmal , Jam Nazir Ahmad

{"title":"Resistance development, cross-resistance, and inheritance patterns in clothianidin and triazophos resistant strains of Oxycarenus hyalinipennis","authors":"Rabia Saeed , Naeem Abbas , Muhammad Akmal , Jam Nazir Ahmad","doi":"10.1016/j.cropro.2025.107197","DOIUrl":null,"url":null,"abstract":"<div><div>The dusky cotton bug, <em>Oxycarenus hyalinipennis</em> Costa (Lygaidae: Hemiptera), is a polyphagous pest and has become one of the most serious sucking pests affecting cotton in Pakistan. <em>O. hyalinipennis</em> has the potential to develop resistance to various insecticides, leading to outbreaks of this pest. Therefore, this study aimed to gather information of insecticide resistance development, cross-resistance pattern, and inheritance mode of resistance to clothianidin and triazophos in laboratory selected strains of <em>O</em>. <em>hyalinipennis</em>. The results showed that after 9 generations, resistant strains developed high levels of resistance to clothianidin (640.97-fold for Clothi-RS3, 1204.06-fold for Clothi-RS2, and 2472.52-fold for Clothi-RS1) and triazophos (236.38-fold for Triaz-RS3, 299.53-fold for Triaz-RS2, and 714.15-fold for Triaz-RS1) compared to the susceptible strains. The extremely high cross-resistance to triazophos (158.42-fold), profenofos (1128.32-fold) and indoxacarb (387.10-fold) in the Clothi-RS1 strain, compared to the respective susceptible strain was observed. Similarly, extremely high cross-resistance to clothianidin (255.90-fold), profenofos (451.33-fold) and indoxacarb (954.79-fold) in the Triaz-RS1 strain was observed. Overlapping 95 % confidence limits of LC<sub>50s</sub> of all reciprocal crosses and degree of dominance values revealed an autosomal and incompletely dominant resistance to clothianidin and triazophos. All backcrosses of the F<sub>1</sub> reciprocal cross with resistant parent strains predicted a polygenic inheritance. In conclusion, clothianidin and triazophos resistance was inherited as autosomal, incompletely dominant, and polygenic in the Cloth-RS and Triaz-RS. The high level of cross-resistance to clothianidin, triazophos, profenofos, and indoxacarb indicates that these pesticides are ineffective in controlling the target pest. These findings provide vital information for sustainable pest management practices.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":"193 ","pages":"Article 107197"},"PeriodicalIF":2.5000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Protection","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0261219425000894","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

The dusky cotton bug, Oxycarenus hyalinipennis Costa (Lygaidae: Hemiptera), is a polyphagous pest and has become one of the most serious sucking pests affecting cotton in Pakistan. O. hyalinipennis has the potential to develop resistance to various insecticides, leading to outbreaks of this pest. Therefore, this study aimed to gather information of insecticide resistance development, cross-resistance pattern, and inheritance mode of resistance to clothianidin and triazophos in laboratory selected strains of O. hyalinipennis. The results showed that after 9 generations, resistant strains developed high levels of resistance to clothianidin (640.97-fold for Clothi-RS3, 1204.06-fold for Clothi-RS2, and 2472.52-fold for Clothi-RS1) and triazophos (236.38-fold for Triaz-RS3, 299.53-fold for Triaz-RS2, and 714.15-fold for Triaz-RS1) compared to the susceptible strains. The extremely high cross-resistance to triazophos (158.42-fold), profenofos (1128.32-fold) and indoxacarb (387.10-fold) in the Clothi-RS1 strain, compared to the respective susceptible strain was observed. Similarly, extremely high cross-resistance to clothianidin (255.90-fold), profenofos (451.33-fold) and indoxacarb (954.79-fold) in the Triaz-RS1 strain was observed. Overlapping 95 % confidence limits of LC_50s of all reciprocal crosses and degree of dominance values revealed an autosomal and incompletely dominant resistance to clothianidin and triazophos. All backcrosses of the F₁ reciprocal cross with resistant parent strains predicted a polygenic inheritance. In conclusion, clothianidin and triazophos resistance was inherited as autosomal, incompletely dominant, and polygenic in the Cloth-RS and Triaz-RS. The high level of cross-resistance to clothianidin, triazophos, profenofos, and indoxacarb indicates that these pesticides are ineffective in controlling the target pest. These findings provide vital information for sustainable pest management practices.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

暗色棉虫 Oxycarenus hyalinipennis Costa（Lygaidae: Hemiptera）是一种多食性害虫，已成为影响巴基斯坦棉花的最严重的吸食害虫之一。O. hyalinipennis 有可能对各种杀虫剂产生抗药性，导致这种害虫的爆发。因此，本研究旨在收集实验室筛选出的 O. hyalinipennis 菌株对杀虫剂的抗性发展、交叉抗性模式以及对克螨啶和三唑磷的抗性遗传模式。结果表明，与易感株系相比，抗性株系在9代后对克线磷和三唑磷产生了很高的抗性（克线磷-RS3为640.97倍，克线磷-RS2为1204.06倍，克线磷-RS1为2472.52倍），三唑磷（三唑磷-RS3为236.38倍，三唑磷-RS2为299.53倍，三唑磷-RS1为714.15倍）。与相应的易感菌株相比，Clothi-RS1 菌株对三唑磷（158.42 倍）、丙溴磷（1128.32 倍）和茚虫威（387.10 倍）具有极高的交叉抗性。同样，在 Triaz-RS1 株系中也观察到了对克线磷（255.90 倍）、丙溴磷（451.33 倍）和茚虫威（954.79 倍）的极高交叉抗性。所有互交LC50s和显性程度值的95%置信区间重叠显示，对克线磷和三唑磷的抗性为常染色体不完全显性抗性。与抗性亲本株系进行的 F1 往复杂交的所有回交均预测为多基因遗传。总之，在 Cloth-RS 和 Triaz-RS 中，克菌丹和三唑磷抗性是常染色体、不完全显性和多基因遗传。对克菌丹、三唑磷、丙溴磷和茚虫威的高度交叉抗性表明，这些农药对目标害虫的防治效果不佳。这些发现为可持续害虫管理实践提供了重要信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.