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Selectivity of pre-emergent herbicides in cassava (Manihot esculenta Crantz) and its relationship with the stem cutting planting system 木薯(Manihot esculenta Crantz)萌芽前除草剂的选择性及其与切茎种植系统的关系
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-15 DOI: 10.1016/j.cropro.2024.106985
Cassava (Manihot esculenta Crantz, Euphorbiaceae), an important source of food for millions of people in the world, is distributed mainly in Asia, Africa, Latin America, and the Caribbean. Weeds are important biotic constraints on cassava production, and herbicide application can provide effective weed control in this crop. However, the different possibilities of herbicide selectivity (factors associated and not associated with the plants) must be studied before implementation. The aim of this study was to evaluate the effects of the combination of different pre-emergence herbicides and two stem cutting planting systems (vertical and horizontal) on phytotoxicity and several agronomic traits of the cassava crop. The experiment was conducted through a factorial arrangement in a completely random design in Corrientes, Argentina. The factors studied were planting systems (horizontal and vertical position), weed control methods (hoeing or application of diuron, S-metolachlor, pendimethalin or clomazone, 625; 1920; 900 and 1000 g a.i. ha−1, respectively), and cropping seasons (2018–2019 and 2019–2020). The following variables were measured: phytotoxicity, plant height, relative plant stand, total weight of tuberous roots per plant, starch concentration of tuberous roots, and starch production per ha. The highest precipitations occurred in the 2018–2019 cropping season. The vertical planting system in combination with herbicides showed lower phytotoxicity values and higher values of plant height, starch concentration, and starch production per ha than the horizontal planting system. The highest phytotoxicity values were recorded with the application of diuron combined with the horizontal planting system and in the 2018–2019 season, with a 33.33% decrease in relative plant stand compared to the use of hoeing. Starch concentration remained unchanged with the use of clomazone and pendimethalin, whereas the effect of these herbicides on starch production varied with the cropping season, decreasing only in 2019–2020. Starch production was significantly lower with the use of S-metolachlor (up to 55.29% in 2018–2019) and of diuron (up to 60.57% in 2019–2020) than with the use of hoeing. In conclusion, phytotoxicity values were higher for the horizontal planting system than for the vertical system. The herbicide with the highest phytotoxic effect on the cassava crop was diuron; the effect increased when combined with the horizontal planting system in the cropping season with higher rainfall. This work provides the basis for integrated weed management concerning the use of herbicides and their selectivity according to planting systems for cassava crops.
木薯(Manihot esculenta Crantz,大戟科)是世界上数百万人的重要食物来源,主要分布在亚洲、非洲、拉丁美洲和加勒比地区。杂草是木薯生产的重要生物制约因素,施用除草剂可有效控制杂草。然而,在使用前必须研究除草剂选择性的不同可能性(与植物相关或无关的因素)。本研究的目的是评估不同的萌芽前除草剂和两种茎切割种植系统(垂直和水平)的组合对木薯作物的植物毒性和若干农艺性状的影响。试验在阿根廷科连特斯以完全随机设计的因子排列方式进行。研究的因素包括种植系统(水平和垂直位置)、杂草控制方法(锄草或施用噻草隆、S-甲草胺、戊唑醇或氯马宗,有效成分分别为 625、1920、900 和 1000 克/公顷)以及种植季节(2018-2019 年和 2019-2020 年)。测量了以下变量:植物毒性、植株高度、相对株高、每株块根总重量、块根淀粉浓度和每公顷淀粉产量。最高降水量出现在 2018-2019 年种植季。与水平种植系统相比,与除草剂结合的垂直种植系统显示出较低的植物毒性值,以及较高的株高、淀粉浓度和每公顷淀粉产量值。植物毒性值最高的是施用噻草隆与水平种植系统相结合的情况,并且是在 2018-2019 年种植季,与锄草相比,相对株高降低了 33.33%。使用氯马宗和戊唑醇后,淀粉浓度保持不变,而这些除草剂对淀粉产量的影响随种植季节而变化,仅在 2019-2020 年有所降低。使用 S-甲草胺(2018-2019 年高达 55.29%)和二嘧磺隆(2019-2020 年高达 60.57%)时,淀粉产量明显低于使用锄草时。总之,水平种植系统的植物毒性值高于垂直种植系统。对木薯作物植物毒性影响最大的除草剂是利谷隆;在降雨量较高的种植季节与水平种植系统结合使用时,植物毒性影响会增加。这项研究为木薯作物除草剂的使用及其根据种植系统的选择性提供了综合杂草管理的基础。
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引用次数: 0
Sustainable management of blister and grey blight diseases of tea using antagonistic and plant growth promoting microbes in Western Ghats of India 在印度西高止山脉利用拮抗微生物和植物生长促进微生物对茶叶水泡病和灰霉病进行可持续管理
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-15 DOI: 10.1016/j.cropro.2024.106984
The blister and grey blights are the devastating leaf diseases in tea. The severity of blister and grey blight diseases in tea ranged from 22% to 68% and 11.41%–57.02%, respectively in a survey conducted in Southern India during 2022–2023. The morphological and molecular identifications of pathogens revealed that Exobasidium vexans and Neopestalotiopsis clavispora were responsible for causing blister and grey blights on tea in southern India. The conventional management strategy with foliar spray of synthetic fungicides result in fungicidal residue in tea causes environmental pollution and health hazards to human. To overcome this the current study aims to manage the blister and grey blight diseases of tea through the microbial biocontrol agents. The bacterial biocontrol agents viz., Bacillus subtilis (BBV57), Bacillus amyloliquefaciens (TNAU), Pseudomonas fluorescens (Pf1) and the fungal antagonist, Trichoderma asperellum (TV 1) were evaluated for their efficacy against the both blight diseases. All the bio agents have significantly reduced the basidiospore germination and the mycelial growth of E. vexans and N. clavispora, respectively. Among the bio agents, B. amyloliquefaciens registered 88.36% reduction in the germination of E. vexans basidiospore and 85.56% of mycelial growth reduction of N. clavispora in vitro (P < 0.05). Under field conditions the combined soil and foliar application of bacterial antagonists viz., B. subtilis, B. amyloliquefaciens and P. fluorescens each with 1 ˟ 1011 CFU/ml @ 5% concentration at 7 days interval significantly reduced the blister blight incidence with 53.53% & 51.53% reduction over control and 51.53% & 43.65% of reduction over initial during first and second year, respectively. The combined application also reduced the grey blight incidence with 53.53% & 52.00% reduction over control and 56.81% & 47.65% of reduction over initial during first and second year, respectively. This treatments with bioagents were almost comparable with synthetic treatments, such as copper oxy chloride (0.25%) and hexaconazole (0.25%) with 64.63% and 64.76% reduction of blister and grey blight reduction in first year; 59.74%, 51.11% reduction in second year over control (P < 0.05), respectively. Thus, the application of B. subtilis, B. amyloliquefaciens and P. fluorescens can be recommended for the sustainable management of tea blister and grey blight diseases in tea gardens.
水泡病和灰霉病是茶叶中毁灭性的叶部病害。2022-2023 年期间在印度南部进行的一项调查显示,茶叶水泡病和灰霉病的严重程度分别为 22% 至 68% 和 11.41% 至 57.02%。病原体的形态学和分子鉴定显示,Exobasidium vexans 和 Neopestalotiopsis clavispora 是造成印度南部茶叶水泡病和灰霉病的罪魁祸首。叶面喷洒合成杀菌剂的传统管理策略会导致杀菌剂残留在茶叶中,造成环境污染并危害人类健康。为了克服这一问题,目前的研究旨在通过微生物生物控制剂来控制茶叶的水疱病和灰疫病。研究评估了细菌生物防治剂,即枯草芽孢杆菌(BBV57)、淀粉芽孢杆菌(TNAU)、荧光假单胞菌(Pf1)和真菌拮抗剂毛霉(TV1)对这两种枯萎病的防治效果。所有生物制剂都分别大大减少了 E. vexans 和 N. clavispora 的基生孢子萌发和菌丝生长。其中,B. amyloliquefaciens 在体外对 E. vexans basidiospore 的萌发有 88.36% 的抑制作用,对 N. clavispora 的菌丝生长有 85.56% 的抑制作用(P < 0.05)。在田间条件下,土壤和叶面联合施用细菌拮抗剂,即枯草芽孢杆菌、淀粉芽孢杆菌和荧光链球菌,每种细菌的浓度为 1 ˟ 1011 CFU/ml @ 5%,每隔 7 天施用一次,能显著降低水疱病的发病率,第一年和第二年分别比对照降低了 53.53% 和 51.53%,比最初降低了 51.53% 和 43.65%。联合施用还降低了灰枯病的发病率,第一年和第二年分别比对照降低了 53.53% 和 52.00%,比初始降低了 56.81% 和 47.65%。使用生物制剂的处理方法与合成处理方法(如氧氯化铜(0.25%)和己唑醇(0.25%))几乎相当,第一年水疱病和灰霉病发病率分别比对照减少 64.63% 和 64.76%;第二年分别比对照减少 59.74% 和 51.11%(P <0.05)。因此,建议在茶园中应用枯草芽孢杆菌、淀粉芽孢杆菌和荧光粉芽孢杆菌对茶叶水泡病和灰霉病进行可持续管理。
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引用次数: 0
Fusarium graminearum causes leaf spot disease in Nicandra physalodes in China 禾本科镰刀菌在中国引起尼古拉尺蠖叶斑病
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-11 DOI: 10.1016/j.cropro.2024.106983
Nicandra physalodes is an annual herbaceous plant that belongs to the Solanaceae family. Though native to South America, it is now extensively cultivated in China for medicinal and ornamental purposes. In traditional Chinese medicine, various parts of the plant are used for their therapeutic properties such as sedatives, expectorants, antipyretics, and antidotes. Furthermore, N. physalodes is considered an edible plant, and its seeds contain pectin, which is used in food production. However, recent observations in Hezhang county, Guizhou province, revealed a prevalent leaf spot disease affecting N. physalodes, leading to a decreased yield. In this study, morphological and molecular analyses, pathogenicity assays, and Koch's postulates were used to identify the fungal pathogens responsible for leaf spot disease in N. physalodes. Fusarium graminearum was confirmed as the causative agent based on morphological and molecular analyses, pathogenicity assays, and Koch's postulates, and this represents the first documented case of F. graminearum infection in N. physalodes. These findings underscore the importance of disease management strategies to mitigate the impact of leaf spot disease on the yield of this valuable medicinal plant.
Nicandra physalodes 是茄科一年生草本植物。虽然原产于南美洲,但目前在中国被广泛种植,用于药用和观赏。在传统中药中,该植物的不同部分具有镇静、祛痰、解热和解毒等治疗功效。此外,N. physalodes 被认为是一种可食用植物,其种子含有果胶,可用于食品生产。然而,最近在贵州省赫章县的观察发现,楠木叶斑病流行,导致产量下降。本研究利用形态学和分子分析、致病性测定和科赫定理来确定引起糯玉米叶斑病的真菌病原。根据形态学和分子分析、致病性测定和科赫推定,禾谷镰孢被确认为致病菌,这也是禾谷镰孢感染禾谷牛叶斑病的首个记录病例。这些发现强调了疾病管理策略对减轻叶斑病对这种珍贵药用植物产量影响的重要性。
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引用次数: 0
Evaluation of endemic entomopathogenic nematodes for managing Colorado potato beetle and tuber-damaging pests in potato 评估地方性昆虫病原线虫对科罗拉多马铃薯甲虫和马铃薯块茎破坏性害虫的防治效果
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-10 DOI: 10.1016/j.cropro.2024.106980
Colorado potato beetle, Leptinotarsa decemlineata, wireworms and white grubs are important pests of potato that are challenging to manage. This study evaluated three endemic entomopathogenic nematodes (EPNs) (Steinernema carpocapsae ‘NY01’, S. feltiae ‘NY04’, and Heterorhabditis bacteriophora ‘Oswego’) as biocontrol agents against L. decemlineata, wireworms and white grubs in New York. The efficacy of individual EPN species and their combinations on L. decemlineata larval mortality was assessed via soil-based bioassays in the laboratory. Additionally, L. decemlineata survival and percentage of tubers damaged by wireworms and white grubs were evaluated in field trials in which combinations of pairs of EPN species were applied to the soil at least one month prior to initiating the experiment. Results from bioassays indicated that among the three EPN species, L. decemlineata late instars were most susceptible to H. bacteriophora ‘Oswego’. Additionally, larval mortality was generally higher (14% on average) using pairs of EPN species compared to single species. Soil applications of pairs of EPN species prior to potato planting did not affect L. decemlineata adult survival either during the summer or following spring. However, tuber damage caused by wireworms and white grubs was reduced by 40% using a combination of H. bacteriophora ‘Oswego’ and S. feltiae ‘NY04’. Overall, EPNs applied in soil have potential as biocontrol agents for soil-dwelling pests like wireworms and white grubs in potato, and their efficacy also may extend beyond a single cropping season, but do not seem to be an effective tool for L. decemlineata management in potato.
科罗拉多马铃薯甲虫(Leptinotarsa decemlineata)、线虫和白蛴螬是马铃薯的重要害虫,治理起来非常困难。本研究评估了纽约三种地方性昆虫病原线虫(EPNs)(Steinernema carpocapsae 'NY01'、S. feltiae 'NY04'和 Heterorhabditis bacteriophora 'Oswego')作为生物控制剂对 L. decemlineata、线虫和白蛴螬的防治效果。在实验室中,通过基于土壤的生物测定,评估了单个 EPN 物种及其组合对致死 L. decemlineata 幼虫的功效。此外,还在田间试验中评估了 L. decemlineata 的存活率以及被线虫和白蛴螬破坏的块茎的百分比。生物测定结果表明,在三种 EPN 物种中,L. decemlineata 晚龄幼虫最容易受到 H. bacteriophora 'Oswego' 的侵害。此外,使用成对的 EPN 物种与单一物种相比,幼虫死亡率普遍较高(平均为 14%)。在马铃薯种植前在土壤中施用成对的 EPN 物种不会影响 L. decemlineata 成虫在夏季或次年春季的存活率。然而,使用 H. bacteriophora 'Oswego' 和 S. feltiae 'NY04' 的组合,线虫和白蛴螬对块茎造成的损害减少了 40%。总之,在土壤中施用 EPNs 有可能成为马铃薯线虫和白蛴螬等土栖害虫的生物控制剂,其功效也可能超过一个作物季节,但似乎不是马铃薯 L. decemlineata 管理的有效工具。
{"title":"Evaluation of endemic entomopathogenic nematodes for managing Colorado potato beetle and tuber-damaging pests in potato","authors":"","doi":"10.1016/j.cropro.2024.106980","DOIUrl":"10.1016/j.cropro.2024.106980","url":null,"abstract":"<div><div>Colorado potato beetle, <em>Leptinotarsa decemlineata</em>, wireworms and white grubs are important pests of potato that are challenging to manage. This study evaluated three endemic entomopathogenic nematodes (EPNs) (<em>Steinernema carpocapsae</em> ‘NY01’, <em>S. feltiae</em> ‘NY04’, and <em>Heterorhabditis bacteriophora</em> ‘Oswego’) as biocontrol agents against <em>L. decemlineata</em>, wireworms and white grubs in New York. The efficacy of individual EPN species and their combinations on <em>L. decemlineata</em> larval mortality was assessed via soil-based bioassays in the laboratory. Additionally, <em>L. decemlineata</em> survival and percentage of tubers damaged by wireworms and white grubs were evaluated in field trials in which combinations of pairs of EPN species were applied to the soil at least one month prior to initiating the experiment. Results from bioassays indicated that among the three EPN species, <em>L. decemlineata</em> late instars were most susceptible to <em>H</em>. <em>bacteriophora</em> ‘Oswego’. Additionally, larval mortality was generally higher (14% on average) using pairs of EPN species compared to single species. Soil applications of pairs of EPN species prior to potato planting did not affect <em>L. decemlineata</em> adult survival either during the summer or following spring. However, tuber damage caused by wireworms and white grubs was reduced by 40% using a combination of <em>H</em>. <em>bacteriophora</em> ‘Oswego’ and <em>S</em>. <em>feltiae</em> ‘NY04’. Overall, EPNs applied in soil have potential as biocontrol agents for soil-dwelling pests like wireworms and white grubs in potato, and their efficacy also may extend beyond a single cropping season, but do not seem to be an effective tool for <em>L. decemlineata</em> management in potato.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anthranilic diamide compatibility with the parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae): Based on Toxicity, Two-sex life table and ecological parameters 蒽酰二胺与寄生虫 Trichogramma chilonis Ishii(膜翅目:Trichogrammatidae)的兼容性:基于毒性、双性生命表和生态参数
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-09 DOI: 10.1016/j.cropro.2024.106979
Egg parasitoid wasps of genus Trichogramma Westwood are effective natural enemies of lepidopteran pests in various crops. However, the efficacy of Trichogramma parasitoids is adversely affected by insecticides applied in the field to manage these lepidopteran pests. To overcome this problem efficiently, there is an urgent need to incorporate insecticide-resistant Trichogramma strains into integrated pest management (IPM) programs. This study assessed the potential of Trichogramma chilonis Ishii to develop chlorantraniliprole resistance and characterized resistance associated fitness costs and stability. Toxicity bioassays were performed on T. chilonis using a dry film residue method. After 39 selections, a 573.64-fold level of resistance to chlorantraniliprole was developed in the resistant strain of T. chilonis (Chlr-R) when compared with a chlorantraniliprole susceptible strain (Chlr-S). Chlorantraniliprole resistance in Chlr-R resulted in a female biased sex-ratio and significantly increased the total life span and longevity of the adult female when compared to the Chlr-S strain. Based on age-stage, two-sex life table all the demographic parameters of Chlr-R increased significantly. These results indicate that T. chilonis can be mass reared and selected for chlorantraniliprole resistance under laboratory conditions, is an important step to improve the in field-based effectiveness and integration of these wasps with chemical control.
Trichogramma Westwood 属卵寄生蜂是各种作物中鳞翅目害虫的有效天敌。然而,田间使用杀虫剂来防治这些鳞翅目害虫时,会对 Trichogramma 寄生蜂的功效产生不利影响。为有效解决这一问题,迫切需要将抗杀虫剂的 Trichogramma 菌株纳入害虫综合治理(IPM)计划。本研究评估了 Trichogramma chilonis Ishii 产生氯虫苯甲酰胺抗性的潜力,并描述了与抗性相关的适应成本和稳定性。采用干膜残留法对 Chilonis 进行了毒性生物测定。经过 39 次筛选,T. chilonis 的抗性菌株(Chlr-R)与氯虫苯甲酰胺易感菌株(Chlr-S)相比,对氯虫苯甲酰胺产生了 573.64 倍的抗性。与 Chlr-S 株系相比,Chlr-R 的抗性导致性别比例偏向雌性,并显著延长了成年雌虫的总寿命和寿命。基于年龄阶段的双性生命表,Chlr-R 的所有人口统计学参数都显著增加。这些结果表明,T. chilonis 可以在实验室条件下进行大规模饲养并筛选出抗氯氰菊酯的品系,这对于提高这些蜂的田间防治效果以及将其与化学防治相结合是非常重要的一步。
{"title":"Anthranilic diamide compatibility with the parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae): Based on Toxicity, Two-sex life table and ecological parameters","authors":"","doi":"10.1016/j.cropro.2024.106979","DOIUrl":"10.1016/j.cropro.2024.106979","url":null,"abstract":"<div><div>Egg parasitoid wasps of genus <em>Trichogramma</em> Westwood are effective natural enemies of lepidopteran pests in various crops. However, the efficacy of <em>Trichogramma</em> parasitoids is adversely affected by insecticides applied in the field to manage these lepidopteran pests. To overcome this problem efficiently, there is an urgent need to incorporate insecticide-resistant <em>Trichogramma</em> strains into integrated pest management (IPM) programs. This study assessed the potential of <em>Trichogramma chilonis</em> Ishii to develop chlorantraniliprole resistance and characterized resistance associated fitness costs and stability. Toxicity bioassays were performed on <em>T. chilonis</em> using a dry film residue method. After 39 selections, a 573.64-fold level of resistance to chlorantraniliprole was developed in the resistant strain of <em>T. chilonis</em> (Chlr-R) when compared with a chlorantraniliprole susceptible strain (Chlr-S). Chlorantraniliprole resistance in Chlr-R resulted in a female biased sex-ratio and significantly increased the total life span and longevity of the adult female when compared to the Chlr-S strain. Based on age-stage, two-sex life table all the demographic parameters of Chlr-R increased significantly. These results indicate that <em>T. chiloni</em>s can be mass reared and selected for chlorantraniliprole resistance under laboratory conditions, is an important step to improve the in field-based effectiveness and integration of these wasps with chemical control.</div></div>","PeriodicalId":10785,"journal":{"name":"Crop Protection","volume":null,"pages":null},"PeriodicalIF":2.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cold storage of Aphidoletes aphidimyza can extend offspring larval development period and improve its predation ability 冷藏蚜虫可延长后代幼虫发育期并提高其捕食能力
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-09 DOI: 10.1016/j.cropro.2024.106976
Aphidoletes aphidimyza (Rondani) (Diptera: Cecidomyiidae) is widely used in aphid biological control, and low-temperature storage helps expand its population. Nonetheless, low-temperature effects on A. aphidimyza are unknown. A. aphidimyza pupae were exposed to three cold storage methods lasting 10, 20, and 30 days, respectively: storing at 7 °C for 4 h then 5 °C for 20 h (T1); storing at 9 °C for 4 h then 5 °C for 20 h (T2); and storing at 5 °C for 24 h (T3). The emergence rate under variable low-temperature storage (T1: 81.98%; T2: 81.28%) was significantly higher than that under constant low-temperature storage (T3: 74.98%). Additionally, the emergence rate decreased markedly with prolonged refrigeration time. Egg production at all three refrigeration temperatures declined significantly compared to the control treatment. It is noteworthy that low-temperature storage can significantly prolong the development time of offspring larvae without affecting the overall developmental period of the offspring. After comparing the content of cold-resistant substances in pupae under different low-temperature storage, the total sugar, glycerol, and trehalose increased with an extended storage time. The lifetime feeding capacity of A. aphidimyza offspring larvae on Myzus persicae (Sulzer) improved after cold storage. The functional response curve was aligned with the Holling Ⅱ model. Compared to the control treatment, both T1, T2, and T3 improved the search ability of 3rd-instar larvae in the offspring after 20 and 30 days of refrigeration. Our study indicated that cold storage of A. aphidimyza can extend offspring larval development period and improve its predation ability.
蚜蝇(Aphidoletes aphidimyza (Rondani))(双翅目:蚜蝇科)被广泛用于蚜虫生物防治,低温储存有助于扩大其种群数量。然而,低温对 A. aphidimyza 的影响尚不清楚。将 A. aphidimyza 蛹分别置于三种低温条件下 10 天、20 天和 30 天:在 7 °C 下存放 4 小时,然后在 5 °C 下存放 20 小时(T1);在 9 °C 下存放 4 小时,然后在 5 °C 下存放 20 小时(T2);在 5 °C 下存放 24 小时(T3)。变低温贮藏条件下的出苗率(T1:81.98%;T2:81.28%)明显高于恒定低温贮藏条件下的出苗率(T3:74.98%)。此外,随着冷藏时间的延长,出壳率也明显下降。与对照处理相比,三种冷藏温度下的产蛋量都明显下降。值得注意的是,低温贮藏能显著延长子代幼虫的发育时间,而不影响子代的整体发育期。比较不同低温贮藏条件下蛹体内抗寒物质的含量,总糖、甘油和三卤糖的含量随着贮藏时间的延长而增加。低温贮藏后,蚜茧蜂后代幼虫对柿小蚜(Sulzer)的终生取食能力有所提高。功能反应曲线与霍林Ⅱ模型一致。与对照处理相比,T1、T2 和 T3 在冷藏 20 天和 30 天后都提高了子代三龄幼虫的搜索能力。我们的研究表明,冷藏蚜虫可延长子代幼虫的发育期,提高其捕食能力。
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引用次数: 0
First report of Colletotrichum siamense and C. tropicale causing anthracnose of Syngonium podophyllum in China 中国首次报道暹罗褐壳斗霉菌和热带壳斗霉菌引起豆荚忍冬炭疽病的病例
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-09 DOI: 10.1016/j.cropro.2024.106978
Anthracnose caused by Colletotrichum genus is an important disease of plants in the world. Syngonium podophyllum is a foliage plant in tropical and subtropical areas of the world. In September 2023, serious symptoms of anthracnose were observed on the leaves of S. podophyllum at a park in Haikou City, China, most of the leaves turn yellow to brown and wither, greatly affecting the ornamental value. Colletotrichum siamense and C. tropicale were identified as pathogens by morphological characterization, phylogenetic analysis using ITS region, GAPDH, ACT and TUB2 sequences and pathogenicity tests. This is the first report of C. siamense and C. tropicale causing anthracnose of S. podophyllum in China.
由 Colletotrichum 属引起的炭疽病是世界上一种重要的植物病害。荚蒾(Syngonium podophyllum)是世界热带和亚热带地区的一种观叶植物。2023 年 9 月,中国海口市某公园的豆荚属植物叶片上出现了严重的炭疽病症状,大部分叶片变黄变褐、枯萎,极大地影响了观赏价值。通过形态特征描述、ITS区、GAPDH、ACT和TUB2序列的系统进化分析以及致病性试验,确定暹罗疣孢菌(Colletotrichum siamense)和热带疣孢菌(C. tropicale)为病原菌。这是中国首次报道 C. siamense 和 C. tropicale 引起豆荚属植物炭疽病。
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引用次数: 0
First report of Colletotrichum gloeosporioides causing severe anthracnose on Parthenocissus tricuspidata in China 在中国首次报告由球形孢子菌(Colletotrichum gloeosporioides)引起三尖杉严重炭疽病的病例
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-09 DOI: 10.1016/j.cropro.2024.106977
Parthenocissus tricuspidata is widely cultivated as an ornamental plant. However, severe anthracnose on their leaves has been observed indicating over 80% infection in Hangzhou, Zhejiang Province. The purpose of this study was to characterize the disease causal agent. Colletotrichum-like fungal strains were isolated from the diseased spots. On MEA, the fungal colony appeared white with abundant and dense aerial mycelia. The selected isolates were identified as C. gloeosporioides and C. siamensethe using multi-gene (ITS, ACT, CAL, GAPDH and TUB2) sequence phylogenetic analysis combined with morphological characteristicsPathogenicity tests were conducted by inoculating conidial suspensions on healthy P. tricuspidata leaves, followed by re-isolation and characterization to fulfill the Koch's postulates. This represents the first report that C. gloeosporioides causes anthracnose on P. tricuspidata in China. The potential impact of the disease deserves further investigation considering its high occurrence for sustainable management.
三裂叶木(Parthenocissus tricuspidata)被广泛栽培为观赏植物。然而,在浙江省杭州市观察到其叶片上有严重的炭疽病,感染率超过 80%。本研究的目的是确定病原菌的特征。从病斑中分离出了类似 Colletotrichum 的真菌菌株。在 MEA 上,真菌菌落呈白色,气生菌丝丰富而密集。通过多基因(ITS、ACT、CAL、GAPDH 和 TUB2)序列系统发育分析,并结合形态特征,将分生孢子悬浮液接种到健康的三尖杉叶片上进行致病性试验,然后重新分离和鉴定,以符合科赫假说。这是中国首次报道球孢子菌引起三尖杉炭疽病。考虑到该病的高发生率,其潜在影响值得进一步研究,以实现可持续管理。
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引用次数: 0
Competitiveness of chia against brassica weeds improves through a narrow spatial arrangement 通过狭窄的空间布局提高菊科杂草的竞争力
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-08 DOI: 10.1016/j.cropro.2024.106973
Chia (Salvia hispanica L.) is a promising crop valued as a sustainable source of omega-3. However, chia yield and quality are reduced because of weed competition and the lack of registered herbicides. This study evaluated how chia spatial arrangement suppresses weed growth and provided management options for this crop. The performance of chia plants was assessed over three growing seasons in field experiments using a factorial design that considered spatial arrangement (narrow and wide), weed management (control without weeds, soil bank brassica (Brassica rapa L.), and sown brassica), and cover crop (with or without a rye (Secale cereale L.) cover crop during chia fallow). Chia and brassica are not complementary, leading to resource competition (relative yield total around 1) and negative effects on each other. A significant decrease in chia grain yield of 0.9% per unit increase in the percentage of weeds in total biomass was observed. As chia had a competitive advantage over brassica, the grain yield reduction was ameliorated with a narrow spatial arrangement. Narrow spatial arrangement promotes faster canopy closure during the early growth stages, decreasing light penetration and suppressing weed growth. The high extinction coefficient of chia (0.91) and the low critical LAI (3.3) contributed to this light interception behavior. Accordingly, to maximize chia productivity and profitability, a narrow spatial arrangement is an effective management strategy to suppress weeds. This study contributes valuable insights into chia-weed interactions and emphasizes the importance of sustainable crop management practices.
奇异籽(Salvia hispanica L.)是一种前景广阔的作物,被视为欧米伽-3 的可持续来源。然而,由于杂草竞争和缺乏注册除草剂,奇异籽的产量和质量都有所下降。本研究评估了奇异籽的空间布局如何抑制杂草生长,并为这种作物提供了管理方案。在三个生长季的田间试验中,采用因子设计评估了奇亚籽植物的表现,该设计考虑了空间布局(窄和宽)、杂草管理(无杂草对照、土库黄花菜(Brassica rapa L.)和播种黄花菜)和覆盖作物(在奇亚籽休耕期间有无黑麦(Secale cereale L.)覆盖作物)。奇亚籽和黄刺玫不能互补,导致资源竞争(相对总产量约为 1)和相互间的负面影响。杂草在总生物量中所占比例每增加一个单位,奇亚籽的谷物产量就会大幅下降 0.9%。由于奇异菊比黄花菜更有竞争优势,谷物减产的情况在狭窄的空间布局下得到了改善。窄空间布局可在早期生长阶段加快冠层闭合,减少光照穿透,抑制杂草生长。奇异果的高消光系数(0.91)和低临界 LAI(3.3)促成了这种截光行为。因此,为了最大限度地提高芝麻的产量和收益,狭窄的空间布局是抑制杂草的有效管理策略。这项研究为了解奇异草与杂草的相互作用提供了宝贵的见解,并强调了可持续作物管理方法的重要性。
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引用次数: 0
Identification of novel target genes for RNAi mediated management of the pest, Fall Armyworm (Spodoptera frugiperda, J. E. Smith) 鉴定以 RNAi 为介导的害虫--秋陆虫(Spodoptera frugiperda, J. E. Smith)--管理的新型靶基因
IF 2.5 2区 农林科学 Q1 AGRONOMY Pub Date : 2024-10-05 DOI: 10.1016/j.cropro.2024.106972
RNA interference (RNAi) of vital insect genes is a potential tool for targeted pest management. However, the selection of the right target genes and its dsRNA delivery method is also a challenge because the RNAi efficacy is known to vary among insect species. Fall Armyworm (FAW; Spodoptera frugiperda) is a polyphagous generalist insect pest that can feed on more than 100 plant species, with a strong preference for maize. In the present study, we evaluated the RNAi efficiency of three vital genes coding for Coatomer Subunit alpha (COPIα), Coatomer Subunit beta (COPIβ) and Glutathione-S-transferase U1 (GSTU1) as potential targets for managing FAW. Real-time PCR analysis showed that the target genes' expression level reduced to 75–80% in FAW larvae, after their continuous oral delivery through diet for 8 days. It also caused significant mortality in larvae (40–50%) and pupae (40–62%), and resulted in deformed intermediates (28–38%) and adults (25–40%). It also affected reproduction as evidenced by an approximately 50% reduction in oviposition. Moreover, exogenous application of dsRNA in maize leaves resulted in significant feeding deterrence by FAW larvae, which enhanced the frequency of cannibalism. The impact of target genes’ dsRNA on the biology and behaviour of FAW suggests their insecticidal potential. Thus, the current study reveals the novel target genes and their utility in RNA based pesticides for sustainable management of FAW.
对昆虫重要基因的 RNA 干扰(RNAi)是一种潜在的害虫定向管理工具。然而,如何选择合适的目标基因及其dsRNA递送方法也是一项挑战,因为已知不同昆虫物种的RNAi功效各不相同。秋陆虫(FAW;Spodoptera frugiperda)是一种多食性通翅目害虫,可取食 100 多种植物,对玉米有强烈的偏好。在本研究中,我们评估了三个重要基因的 RNAi 效能,这三个基因分别编码衣壳聚合物亚基α(COPIα)、衣壳聚合物亚基β(COPIβ)和谷胱甘肽-S-转移酶 U1(GSTU1),它们是治理 FAW 的潜在靶标。实时 PCR 分析表明,通过饮食连续口服这些靶基因 8 天后,FAW 幼虫体内靶基因的表达水平降低了 75-80%。它还导致幼虫(40-50%)和蛹(40-62%)大量死亡,并导致中间体(28-38%)和成虫(25-40%)畸形。它还影响繁殖,表现为产卵量减少约 50%。此外,在玉米叶片中外源施用 dsRNA 还能显著阻止 FAW 幼虫取食,从而提高食人的频率。目标基因dsRNA对FAW生物学和行为的影响表明其具有杀虫潜力。因此,目前的研究揭示了新的靶基因及其在基于 RNA 的杀虫剂中的应用,以实现对 FAW 的可持续管理。
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Crop Protection
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