Harnessing Ogataea parapolymorpha DL-1 alcohol oxidase in Komagataella phaffii for recombinant xylanase expression

IF 5.9 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Bioscience Pub Date : 2025-05-01 Epub Date: 2025-03-12 DOI:10.1016/j.fbio.2025.106346

Shoufeng Wang , Wenjie Cong , Mingxuan Wang , Hualan Zhou , Zhenhai Li , Jiguo Wang , Jianguo Zhang

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Abstract

Xylanase production attracted attention because it was an important biological macromolecular in food industry, pharmaceutical industry, animal feed, biorefinery industry. Komagataella phaffii, as a famous recombinant protein producer, used alcohol oxidase 1 to oxidize methanol for recombinant xylanase expression. However, low affinity of alcohol oxidase 1 to oxygen was considered as a limitation because of requirement of a large amount oxygen supply, resulting low consumption of methanol. In this study, Ogataea parapolymorpha DL-1 alcohol oxidase (OpAOX) was used to substitute K. phaffii alcohol oxidase 1 for recombinant xylanase expression increment by 26.7°% through increasing specific methanol consumption rate. Furtherly, recombinant xylanase expression increased by 44.5°% after pH adjustment. These results were also verified by recombinant green fluorescent protein expression in this K. phaffii strain with 54.3°% increment. Therefore, K. phaffii harboring OpAOX provided new approach of recombinant xylanse production, and demonstrated a general strategy for other food related by-products production by K. phaffii.

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利用法菲Komagataella DL-1醇氧化酶表达重组木聚糖酶

木聚糖酶是一种重要的生物大分子，在食品工业、制药工业、动物饲料、生物炼制工业中备受关注。法菲Komagataella phaffii是著名的重组蛋白生产商，利用乙醇氧化酶1氧化甲醇表达重组木聚糖酶。然而，由于需要大量供氧，酒精氧化酶1对氧的亲和力较低被认为是一个限制，导致甲醇的消耗较低。本研究利用副多形Ogataea parpolymorpha DL-1醇氧化酶（OpAOX）替代K. phaffii醇氧化酶1，通过提高比甲醇消耗率，使重组木聚糖酶的表达量增加26.7°%。pH调整后，重组木聚糖酶的表达量增加了44.5°%。重组绿色荧光蛋白在该菌株中的表达量增加了54.3°%，证实了上述结果。因此，含OpAOX的法菲克氏菌提供了重组木聚糖酶生产的新途径，并为法菲克氏菌生产其他食品相关副产品提供了一般策略。

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.