Intra- and Interbead Communications by an Anchored DNA Structure and Cascaded DNA Reactions.

IF 3.7 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS ACS Synthetic Biology Pub Date : 2025-03-14 DOI:10.1021/acssynbio.4c00709
Ibuki Kawamata, Satoru Yoshizawa, Keita Abe, Masahiro Takinoue, Shin-Ichiro M Nomura, Satoshi Murata
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引用次数: 0

Abstract

In nature, communication between compartments, such as cells and organelles, gives rise to biological complexity. Two types of chemical communication play important roles in achieving this complexity: intra- and intercompartment communication. Building a bioinspired synthetic system that can exhibit such communication is of interest for realizing microscale artificial robots with the complexity of actual cells. In this study, we aimed to demonstrate intra- and interbead communication using microbeads made of hydrogels as compartments. We employed the diffusion and reaction of programmed DNA molecules as a medium for chemical communication. As a result of the reaction-diffusion dynamics of DNA, the spatiotemporal development of fluorophore-labeled DNAs was observed under fluorescence microscopy, showing both intra- and interbead communication. Our simple, robust, and scalable methodology will accelerate the fabrication of synthetic microsystems that may have complex functionalities from various local interactions.

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来源期刊
CiteScore
8.00
自引率
10.60%
发文量
380
审稿时长
6-12 weeks
期刊介绍: The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.
期刊最新文献
Intra- and Interbead Communications by an Anchored DNA Structure and Cascaded DNA Reactions. Advancing VB12 Production: Insights into Enhancing VB12 Titer in Ensifer adhaerens Casida A through ARTP Mutagenesis and Multiomics Analysis. XanthoMoClo─A Robust Modular Cloning Genetic Toolkit for the Genera Xanthobacter and Roseixanthobacter. Semiautomated Production of Cell-Free Biosensors. Synthetic Biology Strategies for the Production of Natural Colorants and Their Non-Natural Derivatives.
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