{"title":"Intra- and Interbead Communications by an Anchored DNA Structure and Cascaded DNA Reactions.","authors":"Ibuki Kawamata, Satoru Yoshizawa, Keita Abe, Masahiro Takinoue, Shin-Ichiro M Nomura, Satoshi Murata","doi":"10.1021/acssynbio.4c00709","DOIUrl":null,"url":null,"abstract":"<p><p>In nature, communication between compartments, such as cells and organelles, gives rise to biological complexity. Two types of chemical communication play important roles in achieving this complexity: intra- and intercompartment communication. Building a bioinspired synthetic system that can exhibit such communication is of interest for realizing microscale artificial robots with the complexity of actual cells. In this study, we aimed to demonstrate intra- and interbead communication using microbeads made of hydrogels as compartments. We employed the diffusion and reaction of programmed DNA molecules as a medium for chemical communication. As a result of the reaction-diffusion dynamics of DNA, the spatiotemporal development of fluorophore-labeled DNAs was observed under fluorescence microscopy, showing both intra- and interbead communication. Our simple, robust, and scalable methodology will accelerate the fabrication of synthetic microsystems that may have complex functionalities from various local interactions.</p>","PeriodicalId":26,"journal":{"name":"ACS Synthetic Biology","volume":" ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Synthetic Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1021/acssynbio.4c00709","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
In nature, communication between compartments, such as cells and organelles, gives rise to biological complexity. Two types of chemical communication play important roles in achieving this complexity: intra- and intercompartment communication. Building a bioinspired synthetic system that can exhibit such communication is of interest for realizing microscale artificial robots with the complexity of actual cells. In this study, we aimed to demonstrate intra- and interbead communication using microbeads made of hydrogels as compartments. We employed the diffusion and reaction of programmed DNA molecules as a medium for chemical communication. As a result of the reaction-diffusion dynamics of DNA, the spatiotemporal development of fluorophore-labeled DNAs was observed under fluorescence microscopy, showing both intra- and interbead communication. Our simple, robust, and scalable methodology will accelerate the fabrication of synthetic microsystems that may have complex functionalities from various local interactions.
期刊介绍:
The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism.
Topics may include, but are not limited to:
Design and optimization of genetic systems
Genetic circuit design and their principles for their organization into programs
Computational methods to aid the design of genetic systems
Experimental methods to quantify genetic parts, circuits, and metabolic fluxes
Genetic parts libraries: their creation, analysis, and ontological representation
Protein engineering including computational design
Metabolic engineering and cellular manufacturing, including biomass conversion
Natural product access, engineering, and production
Creative and innovative applications of cellular programming
Medical applications, tissue engineering, and the programming of therapeutic cells
Minimal cell design and construction
Genomics and genome replacement strategies
Viral engineering
Automated and robotic assembly platforms for synthetic biology
DNA synthesis methodologies
Metagenomics and synthetic metagenomic analysis
Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction
Gene optimization
Methods for genome-scale measurements of transcription and metabolomics
Systems biology and methods to integrate multiple data sources
in vitro and cell-free synthetic biology and molecular programming
Nucleic acid engineering.