Wen Ding, Wu Ye, Huayan Liu, Jianbo Yang, Chengxing Chu, Huancheng Zhu, Jiakang Wang, Luping Zhou, Ming Zhao, Ming Liu
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引用次数: 0
Abstract
Cancer remains the leading cause of death worldwide, and early detection can significantly reduce patient mortality. Circulating tumor cells (CTCs), which are tumor cells shed from the primary tumor and transported to distant sites through the bloodstream, are key biomarkers for cancer diagnosis and contain critical information reflecting the primary tumor, making them important for monitoring cancer progression. Microfluidic chips utilizing a purely physical capture technique based on the size and deformability differences between CTCs and other blood cells have proven to be effective in capturing CTCs. This study investigates three high-gradient microstructured hybrid microfluidic chips (HGMH-Chips), each incorporating a microarray structure and a distinct geometric gradient design: linear, sawtooth, and waveform. Multiphysics simulations revealed significant differences in pressure distribution among the chip configurations. Notably, the sawtooth design exhibited a more uniform pressure drop, with only 25% of the particles in the high-pressure region. We employed two cancer cell lines (MDA-MB-231 and A549) to evaluate the chip's capture capability. Additionally, we compared the capture efficiency and cell viability across the three designs in a single cancer cell system. Experimental results demonstrated that the sawtooth chip achieved a capture efficiency of up to 70%. When applied to mixed samples containing leukocytes, the high-gradient design exhibited a capture purity of up to 98%, effectively isolating a small number of cancer cells from complex samples. This model holds promise for the capture of CTCs in complex systems. Furthermore, the microarray structure aids in stabilizing the captured cancer cells, enhancing separation efficiency. This study presents a novel chip structure design for tumor cell capture, which holds promise for improving the capture of tumor cells in complex biological samples.
期刊介绍:
Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.