Protocol for forming G-quadruplexes from double-stranded DNA during transcription.

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS STAR Protocols Pub Date : 2025-03-13 DOI:10.1016/j.xpro.2025.103677
Jiayu Zhang, Jiayuan Gong, Jun Chen, Hanqing Chen
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引用次数: 0

Abstract

G-quadruplexes are four-stranded nucleic acid structures that play vital roles in regulating gene expression, maintaining genomic stability, and supporting various biological processes. This protocol details their formation from double-stranded DNA via in vitro transcription. It includes steps for selecting suitable DNA templates, assembling necessary components (such as RNA polymerase, nucleotides, and buffers), setting optimal incubation conditions, and performing dimethyl sulfate (DMS) footprinting to analyze the structures. For complete details on the use and execution of this protocol, please refer to Zhang et al.1 and Gong et al.2.

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STAR Protocols
STAR Protocols Biochemistry, Genetics and Molecular Biology-General Biochemistry, Genetics and Molecular Biology
CiteScore
2.00
自引率
0.00%
发文量
789
审稿时长
10 weeks
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