Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3.

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY Annals of clinical and laboratory science Pub Date : 2025-01-01

Yuan Lin, Chaoyan Yin, Yanjing Liu, Donghui Lu, Xiaofen Lian

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引用次数: 0

Abstract

Objective: Chronic diabetic nephropathy (CDN) is one of the common complications of the chronic diabetes. The FGF23/FGFR3-mediated signaling pathway is involved in CDN. Whether miR-190a-3P participates in CDN through regulation of FGFR3 remains to be elucidated. The present study evaluated miR-190a-3P's effect on CDN.

Methods: Mice were divided into a control group (NC group), a CDN group, and CDN+miR-190a-3P antagonist group, followed by analysis of miR-190a-3P and FGFR3 level by qRT-PCR, FGFR3, and Tubulin protein level by Western blot as well as blood glucose, serum creatinine (Cr), and urea nitrogen (BUN) using an automatic biochemical analyzer.

Results: Compared with the NC group, the CDN group had a significantly higher miR-190a-3P level in kidney tissue, while the FGFR3 mRNA level was lower (P<0.01). FGFR3 was a target gene of miR-190a-3P. miR-190a-3P levels in the CDN+miR-190a-3P antagonist group were significantly reduced compared with the CDN group (P<0.05). Meanwhile, FGFR3 protein levels in miR-190a-3P antagonist group were significantly increased (P<0.05). Compared with the NC group, the CDN group showed significantly reduced blood glucose level and elevated BUN and Cr level (P<0.01). However, CDN+miR-190a-3P antagonist group showed significantly increased blood glucose and reduced BUN and Cr level compared with CDN group (P<0.05).

Conclusion: miR-190a-3P can directly bind to the 3'-UTR of FGFR3 mRNA and reduce FGFR3 protein levels, contributing to the occurrence of CDN.

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miR-190a-3P通过调节FGFR3对慢性糖尿病肾病的影响

目的：慢性糖尿病肾病（CDN）是慢性糖尿病的常见并发症之一。FGF23/ fgfr3介导的信号通路参与CDN。miR-190a-3P是否通过调控FGFR3参与CDN还有待阐明。本研究评估了miR-190a-3P对CDN的影响。方法：将小鼠分为对照组（NC组）、CDN组和CDN+miR-190a-3P拮抗剂组，采用qRT-PCR分析miR-190a-3P、FGFR3水平，Western blot分析微管蛋白水平，全自动生化分析仪检测血糖、血清肌酐（Cr）、尿素氮（BUN）水平。结果：与NC组相比，CDN组肾组织中miR-190a-3P水平明显升高，而FGFR3 mRNA水平较低（ppppp）结论：miR-190a-3P可直接结合FGFR3 mRNA的3'-UTR，降低FGFR3蛋白水平，促进CDN的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.