In vitro urease inhibition screening of some edible and medicinal herbs to combat Helicobacter pylori related gastric diseases.

Abstract

Prevalence of gastric diseases caused by Helicobacter Pylori bacteria is very common especially in developing countries. H. pylori is not only responsible for initiating gastric complaints like gastritis and peptic ulcer but may also lead to gastric cancer. The aim of this research study is to explore natural flora that exhibit anti urease potential. For this purpose, fifteen edible and medicinal herbs (Silybium marianum, Fagonia arabica, Nigella sativa, Curcuma longa, Ocimum sanctum, Salvia rosmainus, Hyssopus officinalis, Antrirrhinum majus, Salvia splendens, Tropaeolum majus, Dalbergia sisso, Aloe barbadensis, Abelmoschus esculentus, Cuscuta reflexa, Hibiscus schizopetalus) were screened for anti-urease activity at three different concentration i.e, 25μg/ml, 50μg/ml and 75μg/ml. The results indicated significant outcomes for urease inhibitory activity for all tested medicinal plants. However, F. arabica (87.2±1.47), N. sativa (90.4±0.09), O. sanctum (75.6±0.95), H. officinalis (78.9±0.69), T. maju (87.3±0.14), A. esculentus (90.3±0.86), C. reflexa (94.1±0.92) showed significant results at 75μg/ml when compared to Thiourea. Moreover IC₅₀ values were also calculated for urease inhibitory activity. It can be concluded that utilization of these valuable medicinal plants can not only decrease the prevalence of gastric diseases caused by H. pylori bacteria but a good candidate for therapeutic purposes.