Exploring miR-577 and miR-494-3p as Emerging Biomarkers in Sepsis-Associated Acute Kidney Injury: Diagnostic and Prognostic Perspectives

IF 1.8 4区医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2025-03-16 DOI:10.1111/1348-0421.13210

Lixia Xu, Jingpo Li, Li Li, Qiushuang Zhang, Qiuju Feng, Lijie Bai

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Abstract

Sepsis-associated acute kidney injury (AKI) poses a severe threat to patients' lives and health, making early predictions, intervention, and treatment crucial. This study aims to preliminarily explore the clinical role of miR-577 and miR-494-3p in sepsis-associated AKI.

The study included 70 sepsis patients with AKI, 65 sepsis patients without AKI, and a healthy control group (HC, n = 67) to set baseline miRNA levels. Urinary miR-577 and miR-494-3p levels were measured using qRT-PCR. ROC curves evaluated their diagnostic value for sepsis-associated AKI. Logistic regression analyzed AKI risk factors, while Pearson correlation explored miRNA-clinical indicator links. Cox regression models and KM curves assessed the prognostic value of miRNAs in sepsis-associated AKI patients.

Sepsis-associated AKI patients showed heightened inflammatory markers, renal indicators, and APACHE II scores compared to those without AKI. However, their urinary miR-577 and miR-494-3p levels were notably lower, distinguishing them with high diagnostic value. These miRNAs inversely correlated with inflammatory markers, renal indicators, and severity scores. Logistic regression showed lactate, PCT, BUN, Scr, Cys-C, NGAL, KIM-1, and APACHE II, as risk factors, while miR-577 and miR-494-3p were protective. In deceased sepsis-associated AKI patients, these miRNAs were lower, with higher inflammatory markers, renal indicators, and severity scores. miR-577 and miR-494-3p independently predicted mortality, with lower expressions linked to higher death rates.

miR-577 and miR-494-3p are closely related to sepsis-associated AKI and can serve as potential biomarkers for diagnosis and prognostic assessment.

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探索miR-577和miR-494-3p作为脓毒症相关急性肾损伤的新兴生物标志物：诊断和预后观点

脓毒症相关急性肾损伤（AKI）严重威胁患者的生命和健康，因此早期预测、干预和治疗至关重要。本研究旨在初步探讨miR-577和miR-494-3p在脓毒症相关AKI中的临床作用。该研究包括70例合并AKI的脓毒症患者、65例未合并AKI的脓毒症患者和健康对照组（HC, n = 67），以设定基线miRNA水平。采用qRT-PCR检测尿miR-577和miR-494-3p水平。ROC曲线评估其对败血症相关AKI的诊断价值。Logistic回归分析AKI危险因素，Pearson相关分析mirna与临床指标的联系。Cox回归模型和KM曲线评估了mirna在败血症相关AKI患者中的预后价值。与没有AKI的患者相比，脓毒症相关的AKI患者表现出更高的炎症标志物、肾脏指标和APACHE II评分。但尿中miR-577、miR-494-3p水平明显较低，具有较高的诊断价值。这些mirna与炎症标志物、肾脏指标和严重程度评分呈负相关。Logistic回归显示，乳酸、PCT、BUN、Scr、Cys-C、NGAL、KIM-1和APACHE II是危险因素，而miR-577和miR-494-3p是保护因素。在死亡的败血症相关AKI患者中，这些mirna较低，炎症标志物、肾脏指标和严重程度评分较高。miR-577和miR-494-3p独立预测死亡率，低表达与高死亡率相关。miR-577和miR-494-3p与脓毒症相关的AKI密切相关，可以作为诊断和预后评估的潜在生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.